| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54563-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29665-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26578-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26192-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26501-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26531-50ul | |||
![Immunoprecipitation: BICD1 Antibody [NBP1-78734] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Affinity purified rabbit anti-BICD1 antibody used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78735, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Immunoprecipitation-NBP1-78734-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: BICD1 Antibody [NBP1-78734] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Affinity purified rabbit anti-BICD1 antibody used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78735, which recognizes a downstream epitope.")
![Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.](http://images.novusbio.com/fullsize/PAK1-[p-Ser204]-Antibody-Western-Blot-NBP2-29665-img0001.jpg "Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.")
![Immunohistochemistry: Filamin A Antibody (1B2) [NBP3-26578] - Image of Filamin A Antibody (1B2) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26578_rabbit-filamin-a-mab-1b2-262202414471510.jpg "Immunohistochemistry: Filamin A Antibody (1B2) [NBP3-26578] - Image of Filamin A Antibody (1B2) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: Insulin R/CD220 Antibody (4B2) [NBP3-26192] - Staining of MCF7 Cells with Insulin R/CD220 Antibody (4B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26192_rabbit-insulin-r-cd220-mab-4b2-282202412475118.jpg "Immunocytochemistry/Immunofluorescence: Insulin R/CD220 Antibody (4B2) [NBP3-26192] - Staining of MCF7 Cells with Insulin R/CD220 Antibody (4B2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Immunohistochemistry: Aquaporin-4 Antibody (22F10) [NBP3-26501] - Image of Aquaporin-4 Antibody (22F10) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.33% DAB.](http://images.novusbio.com/fullsize/nbp3-26501_rabbit-aquaporin-4-mab-22f10-2722024845326.jpg "Immunohistochemistry: Aquaporin-4 Antibody (22F10) [NBP3-26501] - Image of Aquaporin-4 Antibody (22F10) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.33% DAB.")
![Immunohistochemistry: SOD2/Mn-SOD [ac Lys68] Antibody (23G5) [NBP3-26531] - Image of SOD2/Mn-SOD [ac Lys68] Antibody (23G5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.69% DAB.](http://images.novusbio.com/fullsize/nbp3-26531_rabbit-sod2-mn-sod-ac-lys-68-mab-23g5-262202414471582.jpg "Immunohistochemistry: SOD2/Mn-SOD [ac Lys68] Antibody (23G5) [NBP3-26531] - Image of SOD2/Mn-SOD [ac Lys68] Antibody (23G5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.69% DAB.")