| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82074-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90209-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43821 | |||
| BSA Free | NBP2-46619-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30465-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43807 | |||
| BSA Free | NBP2-46645-25ul | |||
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Western-Blot-NB100-82074-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Analysis of extracts from Hela cells, treated with N oc or calf intestinal phosphates (CIP), using NFkB p105/p50 Antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Staining of methanol-fixed HeLa cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-[p-Ser337]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82074-img0005.jpg)
![Immunohistochemistry: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NB100-82074.](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-10320252332101.jpg)
![Western Blot: NFkB p105/p50 [p Ser337] Antibody [NB100-82074] - Western blot analysis of extract from HeLa cells, using NB100-82074 (Lane 1 and 2).](http://images.novusbio.com/fullsize/nb100-82074_rabbit-polyclonal-nfkb-p105-p50-p-ser337-antibody-103202523472637.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Western-Blot-NBP2-66978-img0003.jpg "Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-M antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0002.jpg)
![Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Western-Blot-NBP2-75632-img0009.jpg "Western Blot: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of PEN2 on mouse spleen tissue lysate using anti-PEN2 antibody at 1/2,000 dilution.")
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0003.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0008.jpg)
![Flow Cytometry: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of A549 cells with PEN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Flow-Cytometry-NBP2-75632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Staining PEN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunocytochemistry-Immunofluorescence-NBP2-75632-img0002.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human lung cancer tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0004.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0005.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0006.jpg)
![Immunohistochemistry-Paraffin: PSENEN/PEN2 Antibody (JE41-28) [NBP2-75632] - Analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-JE41-28-Immunohistochemistry-Paraffin-NBP2-75632-img0007.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0006.jpg "Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0004.jpg)
![Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Flow-Cytometry-NBP2-67216-img0001.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0003.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.](http://images.novusbio.com/fullsize/Abeta-40-Antibody-Western-Blot-NBP1-44047-img0002.jpg "Western Blot: Abeta 40 Antibody [NBP1-44047] - 1-40(lane 1) and 1-42 (lane 2) ABeta peptides were run on a 16.5% TRIS/tricine gel and transferred to PVDF membrane. The peptide concentration was approximately 0.5ng/lane. The blot was incubated with affinity purified AB40 antibody at 1ug/ml, followed by an oat and rabbit -HRP antibody at 1:40,000 and developed using a standard chemiluminescence method. A single band is present only in lane 1 which contains the ABeta 1-40 peptide and no signal is found in lane 2 which contains the ABeta 1-42 peptide.")
![Immunocytochemistry/Immunofluorescence: Agrin Antibody [NBP1-90209] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90209-img0007.jpg "Immunocytochemistry/Immunofluorescence: Agrin Antibody [NBP1-90209] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human Fallopian tube shows moderate positivity in basement membrane in glandular cells.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0015.jpg)
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0013.jpg)
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0014.jpg)
![Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] - Staining of human colon shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Agrin-Antibody-Immunohistochemistry-Paraffin-NBP1-90209-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CLE7-homolog-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84498-img0008.jpg "Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Western-Blot-NBP2-46619-img0009.jpg "Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0007.jpg)
![Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Western-Blot-NBP2-46619-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat striatum shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Analysis in human epididymis and skeletal muscle tissues using NBP2-30465antibody. Corresponding GSS RNA-seq data are presented for the same tissues](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Analysis in human epididymis and skeletal muscle tissues using NBP2-30465antibody. Corresponding GSS RNA-seq data are presented for the same tissues")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human fallopian tube, gastrointestinal, prostate and skeletal muscle using Anti-GSS antibody NBP2-30465 (A) shows similar protein distribution across tissues to independent antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP2-30465] - Analysis using Anti-GSS antibody NBP2-30465 (A) shows similar pattern to independent antibody NBP2-57177 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP2-30465-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30465-img0009.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0019.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0015.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0016.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0017.jpg)
![Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Western-Blot-NBP2-46645-img0009.jpg "Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Western-Blot-NBP2-46645-img0008.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat striatum shows moderate immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat cerebral cortex shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of rat cerebral cortex shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0004.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of mouse hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-NBP2-46645-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (3013) [NBP2-46645] - Staining of human cerebellum shows positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3013-Immunohistochemistry-Paraffin-NBP2-46645-img0006.jpg)