| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48464 | |||
| Azide and BSA Free | NBP2-54314-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26502-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26697-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26492-50ul | |||
![Western Blot: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849] - Western Blot Analysis of human spleen tissue lysates using CD45RO Antibody (UCHL-1 + T200/797).](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-+-T200-797-Western-Blot-NBP2-44849-img0002.jpg "Western Blot: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849] - Western Blot Analysis of human spleen tissue lysates using CD45RO Antibody (UCHL-1 + T200/797).")
![Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849] - Human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1 + T200/797).](http://images.novusbio.com/fullsize/CD45RO-Antibody-UCHL-1-+-T200-797-Immunohistochemistry-Paraffin-NBP2-44849-img0001.jpg)
![Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-282202411413611.jpg "Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: APP Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa Observed band size: 100 kDa")
![Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] - Staining of Hela Cells with APP Antibody (6F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26502_rabbit-app-mab-6f10-28220241312218.jpg)
![Western Blot: FDFT1 Antibody (10H12) [NBP3-15199] - Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate. All lanes: FDFT1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49, 41, 39, 36, 44 KDa. Observed band size: 50 KDa.](http://images.novusbio.com/fullsize/FDFT1-Antibody-10H12-Western-Blot-NBP3-15199-img0003.jpg "Western Blot: FDFT1 Antibody (10H12) [NBP3-15199] - Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate. All lanes: FDFT1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49, 41, 39, 36, 44 KDa. Observed band size: 50 KDa.")
![Immunohistochemistry: FDFT1 Antibody (10H12) [NBP3-15199] - IHC image of FDFT1 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/FDFT1-Antibody-10H12-Immunohistochemistry-NBP3-15199-img0002.jpg)
![Immunohistochemistry: FDFT1 Antibody (10H12) [NBP3-15199] - IHC image of FDFT1 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/FDFT1-Antibody-10H12-Immunohistochemistry-NBP3-15199-img0001.jpg)
![Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241140940.jpg "Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (2D5) [NBP3-26697] - Staining of HepG2 cells with Caspase-9 Antibody (2D5) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241323614.jpg)
![Flow Cytometry: Caspase-9 Antibody (2D5) [NBP3-26697] - Overlay histogram showing K562 cells stained with Caspase-9 Antibody (2D5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-282202414183649.jpg)
![Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-2722024820398.jpg "Immunohistochemistry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Image of SERCA2 ATPase Antibody (20D1) diluted at 1:50 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.41% DAB.")
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Staining of MCF7 with SERCA2 ATPase Antibody (20D1) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-28220241247516.jpg)
![Flow Cytometry: SERCA2 ATPase Antibody (20D1) [NBP3-26492] - Overlay Peak curve showing Hela cells stained with SERCA2 ATPase Antibody (20D1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26492_rabbit-serca2-atpase-mab-20d1-282202414312788.jpg)
![LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LINGO1 stained by LINGO1 antibody [C1C3] (NBP2-19359) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19359_rabbit-polyclonal-lingo-1-antibody-immunocytochemistry-immunofluorescence-2611202518482321.jpg "LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LINGO1 stained by LINGO1 antibody [C1C3] (NBP2-19359) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LINGO1 antibody [C1C3] (NBP2-19359) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19359_rabbit-polyclonal-lingo-1-antibody-western-blot-2611202518191322.jpg)
![Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunohistochemistry-Paraffin-NB100-68244-img0002.jpg "Immunohistochemistry-Paraffin: IRS2 Antibody [NB100-68244] - Human ovarian carcinoma. Antibody used at a dilution of 1:1,000.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: IRS2 Antibody [NB100-68244] - Detection of Human IRS2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IRS2 antibody NB100-68244 used for IP at 3 mcg/mg lysate. IRS2 was also immunoprecipitated by rabbit anti-IRS2 antibodies NB100-68242 and NB100-68243, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/IRS2-Antibody-Immunoprecipitation-NB100-68244-img0003.jpg)