| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98752-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97138-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90139-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89625-50ul | |||
![Western Blot: BNP Antibody (JE30-94) [NBP3-32088] - Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (NBP3-32088) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: U-2 OS cell lysate (20 ug/Lane) Lane 4: Mouse brain tissue lysate (30 ug/Lane) Lane 5: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 15 kDa Observed band size: 37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32088_rabbit-bnp-mab-je30-94-278202415242679.jpg "Western Blot: BNP Antibody (JE30-94) [NBP3-32088] - Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (NBP3-32088) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: U-2 OS cell lysate (20 ug/Lane) Lane 4: Mouse brain tissue lysate (30 ug/Lane) Lane 5: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 15 kDa Observed band size: 37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BNP Antibody (JE30-94) [NBP3-32088] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-BNP antibody (NBP3-32088) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32088) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32088_rabbit-bnp-mab-je30-94-29820241492684.jpg)
![Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-27820241631653.jpg "Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dynamin Antibody (A1B2-R) [NBP3-32283] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-298202414304193.jpg)
![Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP2-98752-img0001.jpg "Western Blot: Stathmin 1 Antibody [NBP2-98752] - Anti-Stathmin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa")
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human tonsil with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0003.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP2-98752] - Immunochemical staining of human Stathmin 1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-98752-img0002.jpg)
![Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP2-87905-img0003.jpg "Western Blot: NFkB p100/p52 Antibody [NBP2-87905] - Host: Rabbit. Target Name: NFKB2. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0002.jpg)
![Immunohistochemistry: NFkB p100/p52 Antibody [NBP2-87905] - Human Smooth Muscle](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-NBP2-87905-img0001.jpg)
![Immunohistochemistry: Mas Antibody [NBP3-14397] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-NBP3-14397-img0003.jpg "Immunohistochemistry: Mas Antibody [NBP3-14397] - Immunohistochemistry of breast carcinoma")
![Immunohistochemistry-Paraffin: Mas Antibody [NBP3-14397] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, neurons and glia, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-Paraffin-NBP3-14397-img0001.jpg)
![Immunohistochemistry-Paraffin: Mas Antibody [NBP3-14397] - Immunohistochemistry of formalin-fixed, paraffin-embedded human testis after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-Paraffin-NBP3-14397-img0002.jpg)
![Immunohistochemistry: Mas Antibody [NBP3-14397] - Immunohistochemistry of lung, non-small cell carcinoma,](http://images.novusbio.com/fullsize/Mas Antibody-Immunohistochemistry-NBP3-14397-img0004.jpg)
![Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0002.jpg "Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T")
![Western Blot: PITX3 Antibody [NBP2-85481] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0001.jpg)
![Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0003.jpg)
![Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-278202415242637.jpg "Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32287) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-29820241435275.jpg)
![Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-288202412461654.jpg "Western Blot: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Ser404] Antibody (JE47-88) [NBP3-32809] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau antibody (NBP3-32809) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32809) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32809_rabbit-tau-p-ser404-mab-je47-88-29820241492670.jpg)
![Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138] - Immunochemical staining of human TR4/NR2C2 in human skin with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TR4-NR2C2-Antibody-Immunohistochemistry-Paraffin-NBP2-97138-img0003.jpg "Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138] - Immunochemical staining of human TR4/NR2C2 in human skin with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138] - Immunochemical staining of human TR4/NR2C2 in human lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TR4-NR2C2-Antibody-Immunohistochemistry-Paraffin-NBP2-97138-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TR4/NR2C2 Antibody [NBP2-97138] - Immunofluorescence staining of TR4/NR2C2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97138 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-97138_rabbit-polyclonal-tr4-nr2c2-antibody-235202514371332.png)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0004.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0001.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0002.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human colon-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0003.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0004.jpg "Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human pancreas with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0001.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human liver with rabbit monoclonal antibody (1:300)](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0002.jpg)
![Immunohistochemistry-Paraffin: VAP-B Antibody (103) [NBP2-89625] - Staining of human VAPB in human cirrhosis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/VAP-B-Antibody-103-Immunohistochemistry-Paraffin-NBP2-89625-img0003.jpg)
![Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-288202412461665.jpg "Western Blot: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32810) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 ug/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tau [p Thr231] Antibody (PSH01-05) [NBP3-32810] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Tau antibody (NBP3-32810) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32810) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32810_rabbit-tau-p-thr231-mab-psh01-05-298202414161831.jpg)