| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90597-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90230-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56589-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95107-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93144-0.02ml | |||
![Immunohistochemistry-Paraffin: RP1 Antibody [NBP1-90597] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/RP1-Antibody-Immunohistochemistry-Paraffin-NBP1-90597-img0005.jpg "Immunohistochemistry-Paraffin: RP1 Antibody [NBP1-90597] - Staining of human prostate shows no positivity in glandular cells as expected.")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP2-56589] - Staining of human cell line SiHa shows localization to nucleoplasm & cytokinetic bridge.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56589-img0001.jpg "Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP2-56589] - Staining of human cell line SiHa shows localization to nucleoplasm & cytokinetic bridge.")
![Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Western-Blot-NBP3-16738-img0001.jpg "Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Numb antibody (NBP3-16738). Western blot was performed from the immunoprecipitate using Numb (NBP3-16738) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Immunoprecipitation-NBP3-16738-img0002.jpg)
![Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PAK1 Antibody-Western Blot-NBP2-95107-img0001.jpg "Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: PAK1 Antibody - Azide and BSA Free [PAK1] - Western blot analysis of various lysates using PAK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp2-95107_rabbit-polyclonal-pak1-antibody-5220251638053.jpg)
![Western Blot: TRIOBP Antibody [NBP2-93144] - Analysis of extracts of various cell lines, using TRIOBP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.](http://images.novusbio.com/fullsize/TRIOBP-Antibody-Western-Blot-NBP2-93144-img0001.jpg "Western Blot: TRIOBP Antibody [NBP2-93144] - Analysis of extracts of various cell lines, using TRIOBP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.")
![Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33535_rabbit-ras-gap-mab-6w10g6-10122024140418.jpg "Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of lysates from mouse brain, using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33535_rabbit-ras-gap-mab-6w10g6-10122024140449.jpg)
![Western Blot: URM1 Antibody [NB600-469] - Western blot using antibody immunized with recombinant yeast URM1 & yeast lysates expressing URM1-GFP fusion protein & other UBL fusion proteins. Panel A: Ponceau total protein staining Panel B: positions of free GFP or GFP- recombinant proteins after reaction with 1:1,000 Anti-GFP, followed by 1:15,000 HRP Donkey-a-Goat IgG MX. Panel C: Specific reaction with URM1 using 1:1,000 IgG fraction of Rabbit-anti- URM1 (Yeast) followed by 1:15,000 HRP Goat-a-Rabbit IgG MX. All primaries diluted in TTBS buffer with 5% non-fat milk & incubated with membranes overnight at 4C. Yeast lysate proteins separated by SDS-PAGE using 15% gel. Data indicates anti-URM1 is highly specific & does not cross react with other UBLs. Bands in Panel C indicate that URM1 & conjugated URM1 are present in most yeast cell lysates, but at lower levels relative to the URM1-GFP transfected lysate. Chemiluminescence system was used for signal detection (Roche).](http://images.novusbio.com/fullsize/URM1-Antibody-Western-Blot-NB600-469-img0004.jpg "Western Blot: URM1 Antibody [NB600-469] - Western blot using antibody immunized with recombinant yeast URM1 & yeast lysates expressing URM1-GFP fusion protein & other UBL fusion proteins. Panel A: Ponceau total protein staining Panel B: positions of free GFP or GFP- recombinant proteins after reaction with 1:1,000 Anti-GFP, followed by 1:15,000 HRP Donkey-a-Goat IgG MX. Panel C: Specific reaction with URM1 using 1:1,000 IgG fraction of Rabbit-anti- URM1 (Yeast) followed by 1:15,000 HRP Goat-a-Rabbit IgG MX. All primaries diluted in TTBS buffer with 5% non-fat milk & incubated with membranes overnight at 4C. Yeast lysate proteins separated by SDS-PAGE using 15% gel. Data indicates anti-URM1 is highly specific & does not cross react with other UBLs. Bands in Panel C indicate that URM1 & conjugated URM1 are present in most yeast cell lysates, but at lower levels relative to the URM1-GFP transfected lysate. Chemiluminescence system was used for signal detection (Roche).")
![N/A: URM1 Antibody [NB600-469]](http://images.novusbio.com/fullsize/URM1-Antibody-N-A-NB600-469-img0003.jpg)
![Western Blot: DRP2 Antibody [NBP2-16229] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/DRP2-Antibody-Western-Blot-NBP2-16229-img0001.jpg "Western Blot: DRP2 Antibody [NBP2-16229] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:500.")
![Western Blot: BIRC6 Antibody [NBP3-41289] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-41289_rabbit-birc6-pab-612202421121384.jpg "Western Blot: BIRC6 Antibody [NBP3-41289] - Sample: Recombinant protein.")
![Immunoprecipitation:Striatin AntibodyNBP3-29144] -Detection of human Striatin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin antibody NBP3-29144 (lot NBP3-29144-1) used for IP at 6 µg per reaction. Striatin was also immunoprecipitated by rabbit anti-Striatin antibody BL17187. For blotting immunoprecipitated Striatin, NBP3-29144 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29144_rabbit-striatin-pab-3012202416502212.jpg "Immunoprecipitation:Striatin AntibodyNBP3-29144] -Detection of human Striatin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin antibody NBP3-29144 (lot NBP3-29144-1) used for IP at 6 µg per reaction. Striatin was also immunoprecipitated by rabbit anti-Striatin antibody BL17187. For blotting immunoprecipitated Striatin, NBP3-29144 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Nardilysin AntibodyNBP3-30543] -Detection of human NRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRD1 antibody NBP3-30543 (lot NBP3-30543-1) used for IP at 6 µg per reaction. NRD1 was also immunoprecipitated by rabbit anti-NRD1 antibody BL19671. For blotting immunoprecipitated NRD1, NBP3-30543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30543_rabbit-nardilysin-pab-3012202412392179.jpg "Immunoprecipitation:Nardilysin AntibodyNBP3-30543] -Detection of human NRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRD1 antibody NBP3-30543 (lot NBP3-30543-1) used for IP at 6 µg per reaction. NRD1 was also immunoprecipitated by rabbit anti-NRD1 antibody BL19671. For blotting immunoprecipitated NRD1, NBP3-30543 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")