| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90701-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97246-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05924-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06520-50ul | |||
![Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-29202410334038.jpg "Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CD45 Antibody (PSH03-64) [NBP3-32448] - Flow cytometric analysis of human PBMC labeling human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32448, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-392024884642.jpg)
![Western Blot: Otx1 Antibody (JE42-10) [NBP3-32681] - Western blot analysis of Otx1 on different lysates with Rabbit anti-Otx1 antibody (NBP3-32681) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32681_rabbit-otx1-mab-je42-10-278202415212419.jpg "Western Blot: Otx1 Antibody (JE42-10) [NBP3-32681] - Western blot analysis of Otx1 on different lysates with Rabbit anti-Otx1 antibody (NBP3-32681) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32681) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-112-Western-Blot-NBP2-90701-img0003.jpg "Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa")
![Flow Cytometry: Syndecan-4 Antibody (112) [NBP2-90701] - Analysis of Mouse SDC4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SDC4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-112-Flow-Cytometry-NBP2-90701-img0002.jpg)
![Immunohistochemistry-Paraffin: Paralemmin Antibody [NBP2-97246] - Immunochemical staining of human Paralemmin in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Paralemmin-Antibody-Immunohistochemistry-Paraffin-NBP2-97246-img0002.jpg "Immunohistochemistry-Paraffin: Paralemmin Antibody [NBP2-97246] - Immunochemical staining of human Paralemmin in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Paralemmin Antibody [NBP2-97246] - Immunochemical staining of human Paralemmin in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Paralemmin-Antibody-Immunohistochemistry-Paraffin-NBP2-97246-img0001.jpg)
![Western Blot: PMP22 Antibody (JM52-30) [NBP2-67068] - Western blot analysis of PMP22 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PMP22-Antibody-JM52-30-Western-Blot-NBP2-67068-img0005.jpg "Western Blot: PMP22 Antibody (JM52-30) [NBP2-67068] - Western blot analysis of PMP22 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Hyaluronan synthase 1 Antibody [NBP3-05924] - Staining of human HAS1 in human smooth muscle with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05924-img0002.jpg "Immunohistochemistry-Paraffin: Hyaluronan synthase 1 Antibody [NBP3-05924] - Staining of human HAS1 in human smooth muscle with rabbit polyclonal antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Hyaluronan synthase 1 Antibody [NBP3-05924] - staining of human HAS1 in human kidney with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Hyaluronan-synthase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05924-img0001.jpg)
![Immunohistochemistry: FOXE3 Antibody [NBP3-10932] - Immunohistochemical analysis of human muscle.](http://images.novusbio.com/fullsize/FOXE3-Antibody-Immunohistochemistry-NBP3-10932-img0001.jpg "Immunohistochemistry: FOXE3 Antibody [NBP3-10932] - Immunohistochemical analysis of human muscle.")
![Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP3-06520] - Staining of human SLC38A1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-Immunohistochemistry-Paraffin-NBP3-06520-img0002.jpg "Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP3-06520] - Staining of human SLC38A1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP3-06520] - Staining of human SLC38A1 in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-Immunohistochemistry-Paraffin-NBP3-06520-img0001.jpg)
![SDS-Page: Complement C3 Partial Recombinant Protein [H00000718-Q01]](http://images.novusbio.com/fullsize/Complement-C3-Partial-Recombinant-Protein-SDS-Page-H00000718-Q01-img0001.jpg "SDS-Page: Complement C3 Partial Recombinant Protein [H00000718-Q01]")
![Western Blot: eNOS Antibody [NBP3-23180] - Western blot analysis of human umbilical vein endothelial cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, 5 & 7) then the blots were treated with lambda phosphatase (lanes 2, 4, 6 & 8). The blots were probed with anti-endothelial nitric oxide synthase (eNOS) (C-terminal region) (lanes 1 & 2), anti-eNOS (Ser-632) (lanes 3 & 4), anti-eNOS (Ser-1177) (lanes 5 & 6) and anti-eNOS (a.a. 1172-1181) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23180_rabbit-enos-pab-19920231759442.jpg "Western Blot: eNOS Antibody [NBP3-23180] - Western blot analysis of human umbilical vein endothelial cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, 5 & 7) then the blots were treated with lambda phosphatase (lanes 2, 4, 6 & 8). The blots were probed with anti-endothelial nitric oxide synthase (eNOS) (C-terminal region) (lanes 1 & 2), anti-eNOS (Ser-632) (lanes 3 & 4), anti-eNOS (Ser-1177) (lanes 5 & 6) and anti-eNOS (a.a. 1172-1181) (lanes 7 & 8).")
![Western Blot: eNOS [p Ser632] Antibody (M232) [NBP3-23181] - Western blot analysis of human umbilical vein endothelial cells stimulated with Calyculin A and untreated (lanes 1, 3, 5) or treated (lanes 2, 4, 6) with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23181_mouse-enos-p-ser632-mab-m232-11920231665313.jpg "Western Blot: eNOS [p Ser632] Antibody (M232) [NBP3-23181] - Western blot analysis of human umbilical vein endothelial cells stimulated with Calyculin A and untreated (lanes 1, 3, 5) or treated (lanes 2, 4, 6) with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).")
![Western Blot: CRMP2 [p Thr555] Antibody (M539) [NBP3-23270] - Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23270_mouse-crmp2-p-thr555-mab-m539-19920231410129.jpg "Western Blot: CRMP2 [p Thr555] Antibody (M539) [NBP3-23270] - Western blot of rat PC12 cells stimulated with calyculin A (100 nM) for 30 min (lanes 1-4). Then the blot was treated with lambda phosphatase to dephosphorylate CRMP2 (lanes 2 & 4). The blot was probed with rabbit polyclonal anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or mouse monoclonal anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).")