| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94464-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93073-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95255-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59409-50ul | |||
![Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Western-Blot-NBP2-45522-img0006.jpg "Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0005.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0001.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0002.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0003.jpg)
![Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/Estrogen-Receptor-Antibody-2D3-Immunohistochemistry-NBP2-45522-img0004.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0006.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-68846-img0002.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0004.jpg)
![Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.](http://images.novusbio.com/fullsize/ND6-Antibody-Western-Blot-NBP2-94464-img0006.jpg "Western Blot: ND6 Antibody [NBP2-94464] - Analysis of various lysates, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 0.8s.")
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Rat kidney using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0004.jpg)
![Western Blot: ND6 Antibody [NBP2-94464] - Analysis of HepG2, using MT-ND6 antibody at 1:2340 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ND6-Antibody-Western-Blot-NBP2-94464-img0005.jpg)
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Human breast cancer using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0002.jpg)
![Immunohistochemistry-Paraffin: ND6 Antibody [NBP2-94464] - Human liver using MT-ND6 antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ND6-Antibody-Immunohistochemistry-Paraffin-NBP2-94464-img0003.jpg)
![Western Blot: PDCD10 Antibody - Azide and BSA Free [NBP2-93073] - Western blot analysis of various lysates, using PDCD10 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp2-93073_rabbit-polyclonal-pdcd10-antibody-4220251430419.jpg "Western Blot: PDCD10 Antibody - Azide and BSA Free [NBP2-93073] - Western blot analysis of various lysates, using PDCD10 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![Immunohistochemistry-Paraffin: PDCD10 Antibody [NBP2-93073] - Paraffin-embedded rat spleen using PDCD10 .](http://images.novusbio.com/fullsize/PDCD10-Antibody-Immunohistochemistry-Paraffin-NBP2-93073-img0007.jpg)
![Immunohistochemistry-Paraffin: PDCD10 Antibody [NBP2-93073] - Paraffin-embedded human breast cancer using PDCD10 .](http://images.novusbio.com/fullsize/PDCD10-Antibody-Immunohistochemistry-Paraffin-NBP2-93073-img0002.jpg)
![Immunohistochemistry-Paraffin: PDCD10 Antibody [NBP2-93073] - Paraffin-embedded mouse kidney using PDCD10 .](http://images.novusbio.com/fullsize/PDCD10-Antibody-Immunohistochemistry-Paraffin-NBP2-93073-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: PDCD10 Antibody - Azide and BSA Free [NBP2-93073] - Immunofluorescence analysis of PC-12 cells using PDCD10 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93073_rabbit-polyclonal-pdcd10-antibody-42202514264480.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Sodium Potassium ATPase Alpha 1 Antibody-Western Blot-NBP2-95255-img0004.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Western blot analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 antibody (NBP2-95255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded mouse kidney using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human liver cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95255] - Immunohistochemistry of paraffin-embedded human lung cancer using Sodium Potassium ATPase Alpha 1 Rabbit pAb (NBP2-95255) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-95255-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody - Azide and BSA Free [Sodium Potassium ATPase Alpha 1] - Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Sodium Potassium ATPase Alpha 1 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp2-95255_rabbit-polyclonal-sodium-potassium-atpase-alpha-1-antibody-5220251640221.jpg)
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Rat lung, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0005.jpg "Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Rat lung, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of Mouse heart, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0004.jpg)
![Western Blot: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Western blot analysis of extracts of HepG2 cells, using Laminin beta 1 Rabbit mAb (NBP3-16392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Laminin-beta-1-Antibody-6I9T4-Western-Blot-NBP3-16392-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of NIH/3T3 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025126533.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of Hep G2 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025125497.jpg)
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody (6I9T4) [NBP3-16392] - Confocal imaging of C6 cells using Laminin beta 1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16392_rabbit-laminin-beta-1-mab-6i9t4-522025125425.jpg)
![Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Figure c: Downregulation of the ALDH5A1 protein product SSADH in TG1-miR. Western blot analysis. SSADH MW, 57 kDa; Actin MW, 42 kDa. Mean +/- SD, n = 3 independent biological samples. Figure d: Decreased ALDH5A1 mRNA levels in TG1-miR compared to TG1. Q-PCR assays. Mean +/- SD, n = 3 independent biological samples. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Western-Blot-NBP2-59409-img0005.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Figure c: Downregulation of the ALDH5A1 protein product SSADH in TG1-miR. Western blot analysis. SSADH MW, 57 kDa; Actin MW, 42 kDa. Mean +/- SD, n = 3 independent biological samples. Figure d: Decreased ALDH5A1 mRNA levels in TG1-miR compared to TG1. Q-PCR assays. Mean +/- SD, n = 3 independent biological samples. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Immunocytochemistry-Immunofluorescence-NBP2-59409-img0002.jpg)
![Immunohistochemistry: ALDH5A1 Antibody (17F5) [NBP2-59409] - SSADH immunoreactive cells are enriched in proliferative/non-differentiated GBM territories (PHIGH/D-) and rare in non-proliferative/differentiated (PLOW/D+) tumor territories of patients' GBM, as revealed by immunohistochemical staining. HES: hematoxylin and eosin staining. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Immunohistochemistry-NBP2-59409-img0004.jpg)
![Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis in HepG2 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Western-Blot-NBP2-59409-img0001.jpg)
![Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Western-Blot-NBP2-59409-img0003.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Western-Blot-NBP2-70688-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) - Azide and BSA Free [NBP2-70688] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI2D3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70688-img0006.jpg)
![Western Blot: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-288202414191517.jpg "Western Blot: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33085) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-2982024149268.jpg)
![Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Immunocytochemistry analysis of HepG2 cells labeling UQCRC2 with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UQCRC2 antibody (NBP3-33085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-29202410234246.jpg)
![Flow Cytometry: UQCRC2 Antibody (JE32-56) [NBP3-33085] - Flow cytometric analysis of HepG2 cells labeling UQCRC2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33085, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33085_rabbit-uqcrc2-mab-je32-56-29202416372169.jpg)
![Western Blot: Tbp7 Antibody (JE62-72) [NBP3-32997] - Western blot analysis of Tbp7 on different lysates with Rabbit anti-Tbp7 antibody (NBP3-32997) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: U-2 OS cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: Mouse testis tissue lysate (40 ug/Lane) Lane 9: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32997) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32997_rabbit-tbp7-mab-je62-72-288202412493425.jpg "Western Blot: Tbp7 Antibody (JE62-72) [NBP3-32997] - Western blot analysis of Tbp7 on different lysates with Rabbit anti-Tbp7 antibody (NBP3-32997) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: U-2 OS cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: Mouse testis tissue lysate (40 ug/Lane) Lane 9: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32997) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Tbp7 Antibody (JE62-72) [NBP3-32997] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Tbp7 antibody (NBP3-32997) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32997) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32997_rabbit-tbp7-mab-je62-72-298202413585543.jpg)
![Immunocytochemistry/ Immunofluorescence: Tbp7 Antibody (JE62-72) [NBP3-32997] - Immunocytochemistry analysis of U-2 OS cells labeling Tbp7 with Rabbit anti-Tbp7 antibody (NBP3-32997) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tbp7 antibody (NBP3-32997) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32997_rabbit-tbp7-mab-je62-72-2920241114825.jpg)
![Flow Cytometry: Tbp7 Antibody (JE62-72) [NBP3-32997] - Flow cytometric analysis of U-2 OS cells labeling Tbp7. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32997, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32997_rabbit-tbp7-mab-je62-72-29202416372163.jpg)
![Western Blot: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of PSMC4 expression in transfected 293T cell line by PSMC4 monoclonal antibody (M01), clone 3G8.Lane 1: PSMC4 transfected lysate(47.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Western-Blot-H00005704-M01-img0012.jpg "Western Blot: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of PSMC4 expression in transfected 293T cell line by PSMC4 monoclonal antibody (M01), clone 3G8.Lane 1: PSMC4 transfected lysate(47.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of monoclonal antibody to PSMC4 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Immunocytochemistry-Immunofluorescence-H00005704-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Tbp7 Antibody (3G8) [H00005704-M01] - Analysis of monoclonal antibody to PSMC4 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Immunohistochemistry-Paraffin-H00005704-M01-img0009.jpg)
![Western Blot: Tbp7 Antibody (3G8) [H00005704-M01] - PSMC4 monoclonal antibody (M01), clone 3G8 Analysis of PSMC4 expression in HepG2.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Western-Blot-H00005704-M01-img0011.jpg)
![Sandwich ELISA: Tbp7 Antibody (3G8) [H00005704-M01] - Detection limit for recombinant GST tagged PSMC4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Tbp7-Antibody-3G8-Sandwich-ELISA-H00005704-M01-img0010.jpg)