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![Western Blot: S100A/B Antibody (C10) [NBP3-41554] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate Primary Ab: 0.2ug/ml NBP3-41554; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41554_mouse-s100a-b-mab-c10-612202421304347.jpg "Western Blot: S100A/B Antibody (C10) [NBP3-41554] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate Primary Ab: 0.2ug/ml NBP3-41554; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: S100A/B Antibody (C5) [NBP3-41222] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate Primary Ab: 0.2ug/ml NBP3-41222; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41222_mouse-s100a-b-mab-c5-61220242116271.jpg "Western Blot: S100A/B Antibody (C5) [NBP3-41222] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate Primary Ab: 0.2ug/ml NBP3-41222; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: S100A/B Antibody (C5) [NBP3-41222] - Sample: Lane1: HT1080 cell lysate; Lane2: HepG2 cell lysate Primary Ab: 0.2ug/ml NBP3-41222; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41222_mouse-s100a-b-mab-c5-612202421243244.jpg)
![Western Blot: FKBP38 Antibody (7-2#) [NBP3-41692] - Sample: Recombinant FKBP38, Human.](http://images.novusbio.com/fullsize/nbp3-41692_mouse-fkbp38-mab-7-2-6122024212795.jpg "Western Blot: FKBP38 Antibody (7-2#) [NBP3-41692] - Sample: Recombinant FKBP38, Human.")
![Immunohistochemistry: Chromogranin A Antibody (D11-2-R) [NBP3-32185] - Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (NBP3-32185) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32185) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32185_mouse-chromogranin-a-mab-d11-2-r-29820241420967.jpg "Immunohistochemistry: Chromogranin A Antibody (D11-2-R) [NBP3-32185] - Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (NBP3-32185) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32185) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-29202410334038.jpg "Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CD45 Antibody (PSH03-64) [NBP3-32448] - Flow cytometric analysis of human PBMC labeling human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32448, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-392024884642.jpg)
![Western Blot: GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype [NBP3-32767] - Western blot analysis of GluR1 on different lysates with Rabbit anti-GluR1 antibody (NBP3-32767) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 102 kDa Observed band size: 110 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32767) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32767_rabbit-glur1-p-ser831-mab-je44-96-ampa-subtype-288202412461652.jpg "Western Blot: GluR1 [p Ser831] Antibody (JE44-96) - AMPA Subtype [NBP3-32767] - Western blot analysis of GluR1 on different lysates with Rabbit anti-GluR1 antibody (NBP3-32767) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 102 kDa Observed band size: 110 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32767) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Notch-2 Antibody (PSH0-54) [NBP3-32657] - Western blot analysis of Notch-2 on different lysates with Rabbit anti-Notch-2 antibody (NBP3-32657) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: PC-3M cell lysate Lane 4: U-87 MG cell lysate Lane 5: HCT116 cell lysate Lane 6: SW620 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 265 kDa Observed band size: 110 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32657) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32657_rabbit-notch-2-mab-psh0-54-278202415212416.jpg "Western Blot: Notch-2 Antibody (PSH0-54) [NBP3-32657] - Western blot analysis of Notch-2 on different lysates with Rabbit anti-Notch-2 antibody (NBP3-32657) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: PC-3M cell lysate Lane 4: U-87 MG cell lysate Lane 5: HCT116 cell lysate Lane 6: SW620 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 265 kDa Observed band size: 110 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32657) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Glut4 [p Ser488] Antibody (JE43-23) [NBP3-32766] - Western blot analysis of Glut4 on mouse heart tissue lysates (no heat) with Rabbit anti-Glut4 antibody (NBP3-32766) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32766) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32766_rabbit-glut4-p-ser488-mab-je43-23-288202412442949.jpg "Western Blot: Glut4 [p Ser488] Antibody (JE43-23) [NBP3-32766] - Western blot analysis of Glut4 on mouse heart tissue lysates (no heat) with Rabbit anti-Glut4 antibody (NBP3-32766) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32766) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: SYT11 Antibody - BSA Free [NBP3-45769] - Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (SYT11 antibody) at dilution of 1:300](http://images.novusbio.com/fullsize/nbp3-45769_rabbit-syt11-pab-bsa-free-28420251372044.jpg "Western Blot: SYT11 Antibody - BSA Free [NBP3-45769] - Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (SYT11 antibody) at dilution of 1:300")
![Western Blot: AKT1 [p Ser129] Antibody (JE59-50) [NBP3-32748] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32748) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32748_rabbit-akt1-p-ser129-mab-je59-50-27820241631645.jpg "Western Blot: AKT1 [p Ser129] Antibody (JE59-50) [NBP3-32748] - Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (NBP3-32748) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32748) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: GluR2/3 Antibody (JE33-28) [NBP3-32478] - Western blot analysis of GluR2/3 on different lysates with Rabbit anti-GluR2/3 antibody (NBP3-32478) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 99 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32478) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32478_rabbit-glur2-3-mab-je33-28-278202415295517.jpg "Western Blot: GluR2/3 Antibody (JE33-28) [NBP3-32478] - Western blot analysis of GluR2/3 on different lysates with Rabbit anti-GluR2/3 antibody (NBP3-32478) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 99 kDa Observed band size: 99 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32478) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")