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![Western Blot: CSN7b Antibody [NB100-461] - Detection of Human CSN7b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN7b antibody NB100-461 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CSN7b-Antibody-Western-Blot-NB100-461-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: CSN7b Antibody [NB100-461] - Detection of Human CSN7b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN7b antibody NB100-461 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: CSN7b Antibody [NB100-461] - Whole cell lysate (50 ug - E; 10 ug - T) from HEK293T cells with (T) or without (E) with a CSN7b expression construct. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/CSN7b-Antibody-Western-Blot-NB100-461-img0003.jpg)
![Western Blot: Synaptojanin 2 Antibody [NBP2-22314] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22314 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Synaptojanin-2-Antibody-Western-Blot-NBP2-22314-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Synaptojanin 2 Antibody [NBP2-22314] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22314 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Synaptojanin 2 Antibody [NBP2-22314] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22314 used for IP at 6 ug/mg lysate. Synaptojanin 2 was also immunoprecipitated by rabbit anti-Synaptojanin 2 antibody NBP2-22315. For blotting immunoprecipitated Synaptojanin 2, NBP2-22314 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Synaptojanin-2-Antibody-Immunoprecipitation-NBP2-22314-img0002.jpg)
![Western Blot: SRGAP1 Antibody [NB100-60650] - Detection of Human SRGAP1/ARHGAP13 on HeLa whole cell lysate using NB100-60650. SRGAP1/ARHGAP13 was also IPed by NB100-60649.](http://images.novusbio.com/fullsize/SRGAP1-Antibody-Western-Blot-NB100-60650-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SRGAP1 Antibody [NB100-60650] - Detection of Human SRGAP1/ARHGAP13 on HeLa whole cell lysate using NB100-60650. SRGAP1/ARHGAP13 was also IPed by NB100-60649.")
![Western Blot: COP9 Antibody [NB100-365] - Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN3 antibody NB100-365 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NB100-365-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: COP9 Antibody [NB100-365] - Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN3 antibody NB100-365 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: COP9 Antibody [NB100-365] - Whole cell lysate (50 ug - E; 10 ug - T) from mock transfected (E) or CSN3 transfected (T) HEK293T cells. Antibody used at the indicated concentration.](http://images.novusbio.com/fullsize/COP9-Antibody-Western-Blot-NB100-365-img0002.jpg)
![Western Blot: Synaptojanin 2 Antibody [NBP2-22315] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Synaptojanin-2-Antibody-Western-Blot-NBP2-22315-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Synaptojanin 2 Antibody [NBP2-22315] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Synaptojanin 2 Antibody [NBP2-22315] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22315 used for IP at 6 ug/mg lysate. Synaptojanin 2 was also immunoprecipitated by rabbit anti-Synaptojanin 2 antibody NBP2-22314. For blotting immunoprecipitated Synaptojanin 2, NBP2-22315 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Synaptojanin-2-Antibody-Immunoprecipitation-NBP2-22315-img0002.jpg)
![Western Blot: Kinesin 5B Antibody [NBP2-32279] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat and cells. Antibodies: Affinity purified rabbit anti-Kinesin 5B antibody NBP2-32279 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Kinesin-5B-Antibody-Western-Blot-NBP2-32279-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Kinesin 5B Antibody [NBP2-32279] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat and cells. Antibodies: Affinity purified rabbit anti-Kinesin 5B antibody NBP2-32279 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Kinesin 5B Antibody [NBP2-32279] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-Kinesin 5B antibody NBP2-32279 used for IP at 6 ug per reaction. For blotting immunoprecipitated Kinesin 5B, NBP2-32279 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Kinesin-5B-Antibody-Immunoprecipitation-NBP2-32279-img0002.jpg)
![Western Blot: GABA-A R beta 3 Antibody [NBP3-10363] - Western blot analysis using NBP3-10363 on Human Liver as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/GABA-A-R-beta-3-Antibody-Western-Blot-NBP3-10363-img0001.jpg "Western Blot: GABA-A R beta 3 Antibody [NBP3-10363] - Western blot analysis using NBP3-10363 on Human Liver as a positive control. Antibody Titration: 0.2-1 ug/ml")
![Western Blot: NMDAR2A Antibody [NBP3-10351] - WB Suggested Anti-NMDAR2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/NMDAR2A Antibody-Western Blot-NBP3-10351-img0001.jpg "Western Blot: NMDAR2A Antibody [NBP3-10351] - WB Suggested Anti-NMDAR2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle")
![Western Blot: PPP2R5C Antibody [NBP3-30779] - Western blot analysis using anti-PP2A/B gamma 2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2).](http://images.novusbio.com/fullsize/nbp3-30779_rabbit-ppp2r5c-pab-811202420592180.jpg "Western Blot: PPP2R5C Antibody [NBP3-30779] - Western blot analysis using anti-PP2A/B gamma 2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2).")