| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92650-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94573-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92455-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81091-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26605-50ul | |||
![Western Blot: LRRK2 [p Ser935] Antibody (4J10Z7) [NBP3-16928] - Western blot analysis of extracts of NIH/3T3 cells, using LRRK2 Rabbit mAb (NBP3-16928) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LRRK2-p-Ser935-Antibody-4J10Z7-Western-Blot-NBP3-16928-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: LRRK2 [p Ser935] Antibody (4J10Z7) [NBP3-16928] - Western blot analysis of extracts of NIH/3T3 cells, using LRRK2 Rabbit mAb (NBP3-16928) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.")
![<b>Biological Strategies Validation. </b>Western Blot: LRRK2 [p Ser935] Antibody (4J10Z7) [NBP3-16928] - Western blot analysis of extracts of C6 cells, using LRRK2 Rabbit mAb (NBP3-16928) at 1:1000 dilution.C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LRRK2-p-Ser935-Antibody-4J10Z7-Western-Blot-NBP3-16928-img0002.jpg)
![Western Blot: Apolipoprotein D Antibody [NBP2-92650] - Analysis of extracts of various cell lines, using Apolipoprotein D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Western-Blot-NBP2-92650-img0002.jpg "Western Blot: Apolipoprotein D Antibody [NBP2-92650] - Analysis of extracts of various cell lines, using Apolipoprotein D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: NMDAR2D Antibody [NBP2-94573] - Analysis of extracts of various cell lines, using NMDAR2D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/NMDAR2D-Antibody-Western-Blot-NBP2-94573-img0001.jpg "Western Blot: NMDAR2D Antibody [NBP2-94573] - Analysis of extracts of various cell lines, using NMDAR2D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Western Blot: NFkB p105/p50 Overexpression Lysate (Adult Normal) [NBL1-13619] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFkB p105/p50.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13619-img0002.jpg "Western Blot: NFkB p105/p50 Overexpression Lysate (Adult Normal) [NBL1-13619] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFkB p105/p50.")
![Western Blot: NFkB p105/p50 [p Ser932] Antibody (8B9G6) [NBP3-33571] - Western blot analysis of various lysates, using NFkB p105/p50 Rabbit mAb at1:2000 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 10 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33571_rabbit-nfkb-p105-p50-p-ser932-mab-8b9g6-101220241233843.jpg "Western Blot: NFkB p105/p50 [p Ser932] Antibody (8B9G6) [NBP3-33571] - Western blot analysis of various lysates, using NFkB p105/p50 Rabbit mAb at1:2000 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 10 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: NFkB p105/p50 [p Ser932] Antibody (8B9G6) [NBP3-33571] - Western blot analysis of various lysates, using NFkB p105/p50 Rabbit mAb at1:2000 dilution. NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) and Calyculin A (100 nM) at 37C for 10 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33571_rabbit-nfkb-p105-p50-p-ser932-mab-8b9g6-101220241224153.jpg)
![Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit mAb (NBP3-16236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-5N2N9-Western-Blot-NBP3-16236-img0001.jpg "Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit mAb (NBP3-16236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of lysates from wild type (WT) and ATG16L1 knockout (KO) HeLa cells using [KO Validated] ATG16L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16236_rabbit-atg16l1-mab-5n2n9-5220251215428.jpg)
![Western Blot: C1QB Antibody - BSA Free [NBP2-92455] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with C1QB using C1QB Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp2-92455_rabbit-polyclonal-c1qb-antibody-42202514264458.jpg "Western Blot: C1QB Antibody - BSA Free [NBP2-92455] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with C1QB using C1QB Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Western Blot: Aquaporin-4 Antibody [NBP2-87018] - WB Suggested Anti-AQP4 Antibody. Titration: 1.0 ug/ml. Positive Control: MDA-MB-435S Whole CellAQP4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-87018-img0001.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-87018] - WB Suggested Anti-AQP4 Antibody. Titration: 1.0 ug/ml. Positive Control: MDA-MB-435S Whole CellAQP4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells")
![Western Blot: Aquaporin-4 Antibody [NBP2-87018] - Host: Mouse. Target Name: AQP4. Sample Tissue: Mouse Heart. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-87018-img0002.jpg)
![Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunofluorescence analysis of paraffin-embedded AD mouse brain tissue labeling Abeta 42 with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32078, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32078_rabbit-abeta-42-mab-psh02-83-298202414161849.jpg "Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunofluorescence analysis of paraffin-embedded AD mouse brain tissue labeling Abeta 42 with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32078, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: Abeta 42 Antibody (PSH02-83) [NBP3-32078] - Immunohistochemical analysis of paraffin-embedded mouse model of Alzheimer's brain tissue with Rabbit anti-Abeta 42 antibody (NBP3-32078) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32078) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32078_rabbit-abeta-42-mab-psh02-83-298202413585551.jpg)
![Immunocytochemistry /Immunofluorescence: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43560 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom: NBP3-43560, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43560_rabbit-aquaporin-4-mab-d15107-chimeric-azide-bsa-free-74202522141214.png "Immunocytochemistry /Immunofluorescence: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43560 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom: NBP3-43560, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.")
![Western Blot: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Unboiled (A) and boiled (B) mouse skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43560 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43560_rabbit-aquaporin-4-mab-d15107-chimeric-azide-bsa-free-74202522113012.png)
![Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate. All lanes: EGR2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26605_rabbit-egr2-mab-27c3-2822024115250.jpg "Western Blot: EGR2 Antibody (27C3) [NBP3-26605] - Positive Western Blot detected in: SY5Y whole cell lysate, U251 whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, Mouse Brain tissue lysate, Mouse Brain tissue lysate. All lanes: EGR2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa")
![Flow Cytometry: EGR2 Antibody (27C3) [NBP3-26605] - Overlay Peak curve showing SH-SY5Y cells stained with EGR2 Antibody (27C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26605_rabbit-egr2-mab-27c3-282202414312741.jpg)