| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50447-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB38332-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB38331-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11980-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48364 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11784-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65366-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65336-0.025mg | |||
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-278202416331211.jpg "Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32145) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-298202414115443.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31/PECAM-1 with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-29202410484878.jpg)
![Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (RA3-6B2) - Chimeric [NBP3-11980] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (NBP3-11980) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11980, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-RA3-6B2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11980-img0001.jpg "Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (RA3-6B2) - Chimeric [NBP3-11980] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RA3-6B2 (NBP3-11980) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11980, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: B220/CD45R Antibody (RA3-6B2) - Chimeric [NBP3-11980] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of RA3-6B2 (NBP3-11980, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-RA3-6B2-Chimeric-Flow-Cytometry-NBP3-11980-img0002.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Sample Type: Human Fetal Muscle Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/lane Gel Concentration: 0.12](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-59186-img0006.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Sample Type: Human Fetal Muscle Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/lane Gel Concentration: 0.12")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Human thymus tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-59186-img0005.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-59186-img0004.jpg)
![Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/Ly-6G-Antibody-RM0064-6D17-Immunohistochemistry-Paraffin-NBP2-11784-img0001.jpg "Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.")
![Flow Cytometry: CD45 Antibody (YW62.3) [NB100-65366] - Analysis using the FITC conjugate of CD45 antibody (YW62.3) [NB100-65366]. Staining of Mouse splenocytes.](http://images.novusbio.com/fullsize/CD45-Antibody-YW62-3-Flow-Cytometry-NB100-65366-img0006.jpg "Flow Cytometry: CD45 Antibody (YW62.3) [NB100-65366] - Analysis using the FITC conjugate of CD45 antibody (YW62.3) [NB100-65366]. Staining of Mouse splenocytes.")
![Flow Cytometry: CD45 Antibody (YW62.3) [NB100-65366] - Staining of mouse spleen cells with CD45 antibody (YW62.3) [NB100-65366].](http://images.novusbio.com/fullsize/CD45-Antibody-YW62-3-Flow-Cytometry-NB100-65366-img0003.jpg)
![Flow Cytometry: CD45 Antibody (YW62.3) [NB100-65366] - Staining of mouse spleen cells with the PE-conjugated version of CD45 antibody (YW62.3) [NB100-65366].](http://images.novusbio.com/fullsize/CD45-Antibody-YW62-3-Flow-Cytometry-NB100-65366-img0005.jpg)
![Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD31-PECAM1-Antibody-LCI-4-Flow-Cytometry-NB100-65336-img0001.jpg "Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.")