| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44854 | |||
| Azide and BSA Free | NBP2-34804-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB38332-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB72272-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB38331-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50447-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11784-0.025mg | |||
![Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution.<br/><br/> Lane 1: THP-1 cell lysate<br/> Lane 2: Jurkat cell lysate<br/> Lane 3: U-937 cell lysate<br/> Lane 4: HeLa cell lysate (negative)<br/> Lane 5: MDA-MB-231 cell lysate (negative)<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 83 kDa<br/> Observed band size: 130 kDa<br/><br/> Exposure time: 1 minute 58 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-278202416331211.jpg "Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution.<br/><br/> Lane 1: THP-1 cell lysate<br/> Lane 2: Jurkat cell lysate<br/> Lane 3: U-937 cell lysate<br/> Lane 4: HeLa cell lysate (negative)<br/> Lane 5: MDA-MB-231 cell lysate (negative)<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 83 kDa<br/> Observed band size: 130 kDa<br/><br/> Exposure time: 1 minute 58 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32145) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-298202414115443.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31/PECAM-1 with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-29202410484878.jpg)
![ELISA: Human CD11b ELISA Kit (Colorimetric) [NBP3-39957] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39957_human-cd11b-elisa-kit-colorimetric-2110202415292750.png "ELISA: Human CD11b ELISA Kit (Colorimetric) [NBP3-39957] - Standard Curve Reference")
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (SPM570) [NBP2-32935] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 antibody (SPM570) [NBP2-32935].](http://images.novusbio.com/fullsize/CD45-Antibody-SPM570-Immunohistochemistry-Paraffin-NBP2-32935-img0003.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (SPM570) [NBP2-32935] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 antibody (SPM570) [NBP2-32935].")
![Immunohistochemistry-Paraffin: CD45 Antibody (SPM570) [NBP2-32935] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45 antibody (SPM570).](http://images.novusbio.com/fullsize/nbp2-32935_mouse-monoclonal-cd45-antibody-spm570-17720241044627.jpg)
![Flow Cytometry: CD14 Antibody (CAM36A) [NBP2-60837] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/CD14-Antibody-CAM36A-Flow-Cytometry-NBP2-60837-img0001.jpg "Flow Cytometry: CD14 Antibody (CAM36A) [NBP2-60837] - Obtained with bovine leukocytes")
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Sample Type: Human Fetal Muscle Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/lane Gel Concentration: 0.12](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-59186-img0006.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Sample Type: Human Fetal Muscle Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/lane Gel Concentration: 0.12")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Human thymus tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-59186-img0005.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-59186] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-59186-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436] - Hooded seal bronchoalveolar lavage cells were stained with Mouse Anti-Human CD14-UNLB followed by a secondary antibody and DRAQ5. Image from Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186.](http://images.novusbio.com/fullsize/CD14-Antibody-61D3-Immunocytochemistry-Immunofluorescence-NBP1-28436-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436] - Hooded seal bronchoalveolar lavage cells were stained with Mouse Anti-Human CD14-UNLB followed by a secondary antibody and DRAQ5. Image from Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186.")
![Flow Cytometry: CD14 Antibody (61D3) [NBP1-28436] - Analysis using the Allophycocyanin conjugate of NBP1-28436. Multiple staining of peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD14-Antibody-61D3-Flow-Cytometry-NBP1-28436-img0002.jpg)
![Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/Ly-6G-Antibody-RM0064-6D17-Immunohistochemistry-Paraffin-NBP2-11784-img0001.jpg "Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.")