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![Western Blot: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Western blot detection of HSP70/HSPA1A in Hela cell lysates using NBP3-19657 (1:1000 diluted). Predicted band size: 70kDa. Observed band size: 70kDa.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-S01-6H6-Western-Blot-NBP3-19657-img0002.jpg "Western Blot: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Western blot detection of HSP70/HSPA1A in Hela cell lysates using NBP3-19657 (1:1000 diluted). Predicted band size: 70kDa. Observed band size: 70kDa.")
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Immunohistochemistry of HSP70/HSPA1A in paraffin-embedded Human tonsil using NBP3-19657 at dilution 1/50](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-S01-6H6-Immunohistochemistry-Paraffin-NBP3-19657-img0001.jpg)
![Western Blot: Dynamin Antibody (SR2307) [NBP3-21813] - Western blot analysis using NBP3-21813 in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21813_rabbit-dynamin-mab-sr2307-6720239533784.jpg "Western Blot: Dynamin Antibody (SR2307) [NBP3-21813] - Western blot analysis using NBP3-21813 in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: FKBP52/FKBP4 Antibody (S08-4F8) [NBP3-19864] - Western blot detection of FKBP52/FKBP4 in U2OS, C6 using NBP3-19864 (1:1000 diluted)](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-S08-4F8-Western-Blot-NBP3-19864-img0002.jpg "Western Blot: FKBP52/FKBP4 Antibody (S08-4F8) [NBP3-19864] - Western blot detection of FKBP52/FKBP4 in U2OS, C6 using NBP3-19864 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody (S08-4F8) [NBP3-19864] - Immunohistochemistry of FKBP52/FKBP4 in paraffin-embedded Human lung cancer tissue using NBP3-19864 at dilution 1/50](http://images.novusbio.com/fullsize/FKBP52-FKBP4-Antibody-S08-4F8-Immunohistochemistry-Paraffin-NBP3-19864-img0001.jpg)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Western blot detection of Clathrin Heavy Chain 1/CHC17 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19848 (1:1000 diluted). Predicted band size: 192kDa. Observed band size: 192kDa.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-S02-6C4-Western-Blot-NBP3-19848-img0002.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Western blot detection of Clathrin Heavy Chain 1/CHC17 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19848 (1:1000 diluted). Predicted band size: 192kDa. Observed band size: 192kDa.")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Immunohistochemistry of Clathrin Heavy Chain 1/CHC17 in paraffin-embedded Human colon cancer tissue using NBP3-19848 at dilution 1/50](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-S02-6C4-Immunohistochemistry-Paraffin-NBP3-19848-img0001.jpg)
![Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/Dynamin-[p-Ser774]-Antibody-Western-Blot-NB300-209-img0002.jpg "Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924252.png "Flow Cytometry: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-16D-6B9 (NBP3-20094, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (SAIC-16D-6B9) - Azide and BSA Free [NBP3-20094] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-16D-6B9 (NBP3-20094) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20094, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20094_rabbit-calreticulin-mab-saic-16d-6b9-azide-and-bsa-free-2742023924253.png)
![Western Blot: Syntaxin 12 Antibody (3B9) [H00023673-M01] - Analysis of STX12 expression in transfected 293T cell line by STX12 monoclonal antibody (M01), clone 3B9.Lane 1: STX12 transfected lysate(31.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-12-Antibody-3B9-Western-Blot-H00023673-M01-img0003.jpg "Western Blot: Syntaxin 12 Antibody (3B9) [H00023673-M01] - Analysis of STX12 expression in transfected 293T cell line by STX12 monoclonal antibody (M01), clone 3B9.Lane 1: STX12 transfected lysate(31.6 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: Syntaxin 12 Antibody (3B9) [H00023673-M01] - Analysis of STX12 transfected lysate using anti-STX12 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STX12 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Syntaxin-12-Antibody-3B9-Immunoprecipitation-H00023673-M01-img0001.jpg)
![Sandwich ELISA: Syntaxin 12 Antibody (3B9) [H00023673-M01] - Detection limit for recombinant GST tagged STX12 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-12-Antibody-3B9-Sandwich-ELISA-H00023673-M01-img0002.jpg)
![Western Blot: TXNRD2 Antibody (PSH02-27) [NBP3-33069] - Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (NBP3-33069) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Lane 9: Human kidney tissue lysate (40 ug/Lane) Lane 10: Mouse kidney tissue lysate (40 ug/Lane) Lane 11: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33069) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33069_rabbit-txnrd2-mab-psh02-27-288202414191514.jpg "Western Blot: TXNRD2 Antibody (PSH02-27) [NBP3-33069] - Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (NBP3-33069) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat heart tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Lane 9: Human kidney tissue lysate (40 ug/Lane) Lane 10: Mouse kidney tissue lysate (40 ug/Lane) Lane 11: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33069) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TXNRD2 Antibody (PSH02-27) [NBP3-33069] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TXNRD2 antibody (NBP3-33069) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33069) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33069_rabbit-txnrd2-mab-psh02-27-29820241420966.jpg)
![Western Blot: Complexin-1 Antibody [NBP3-41341] - Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml NBP3-41341; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41341_rabbit-complexin-1-pab-61220242055471.jpg "Western Blot: Complexin-1 Antibody [NBP3-41341] - Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml NBP3-41341; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;")
![Western Blot: Complexin-1 Antibody [NBP3-41341] - Sample: Recombinant Complexin-1, Human.](http://images.novusbio.com/fullsize/nbp3-41341_rabbit-complexin-1-pab-612202421304387.jpg)
![Western Blot: Complexin-1 Antibody [NBP3-41341] - Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml NBP3-41341; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41341_rabbit-complexin-1-pab-61220242055481.jpg)
![Western Blot: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365] - Western blot analysis of Gamma Adaptin on different lysates with Rabbit anti-Gamma Adaptin antibody (NBP3-32365) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: RAW264.7 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse kidney tissue lysate (40 µg/Lane) Lane 12: Mouse liver tissue lysate (40 µg/Lane) Lane 13: Rat kidney tissue lysate (40 µg/Lane) Lane 14: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32365_rabbit-gamma-adaptin-mab-psh02-65-278202416331245.jpg "Western Blot: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365] - Western blot analysis of Gamma Adaptin on different lysates with Rabbit anti-Gamma Adaptin antibody (NBP3-32365) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: RAW264.7 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse kidney tissue lysate (40 µg/Lane) Lane 12: Mouse liver tissue lysate (40 µg/Lane) Lane 13: Rat kidney tissue lysate (40 µg/Lane) Lane 14: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Gamma Adaptin Antibody (PSH02-65) [NBP3-32365] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Gamma Adaptin antibody (NBP3-32365) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32365) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32365_rabbit-gamma-adaptin-mab-psh02-65-2982024143523.jpg)
![Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP3-45669] - Immunohistochemistry of paraffin-embedded human liver using ( Ubiquitin C Antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-45669_rabbit-ubiquitin-c-pab-bsa-free-284202511414823.jpg "Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP3-45669] - Immunohistochemistry of paraffin-embedded human liver using ( Ubiquitin C Antibody) at dilution of 1:100")
![Western Blot: Ubiquitin C Antibody - BSA Free [NBP3-45669] - HepG2 cells were subjected to SDS PAGE followed by western blot with ( Ubiquitin C Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-45669_rabbit-ubiquitin-c-pab-bsa-free-284202511425529.jpg)