| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37978-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12877-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12876-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97473-0.2mg | |||
| BSA Free | NBP1-97473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90208-50ul | |||
![Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478] - Western blot analysis of extracts of various cell lines, using p97/VCP Rabbit mAb (NBP3-15478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-3P8T3-Western-Blot-NBP3-15478-img0004.jpg "Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478] - Western blot analysis of extracts of various cell lines, using p97/VCP Rabbit mAb (NBP3-15478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] - Confocal imaging of U-2 OS cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15478_rabbit-p97-vcp-mab-3p8t3-immunocytochemistry-immunofluorescence-25102024123752..png)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -Confocal imaging of PC-12 cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15478_rabbit-p97-vcp-mab-3p8t3-immunocytochemistry-immunofluorescence-25102024123938.png)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -Confocal imaging of NIH/3T3 cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15478_rabbit-p97-vcp-mab-3p8t3-immunocytochemistry-immunofluorescence-25102024124410..png)
![Western Blot: SEC61B Antibody [NBP3-37978] - Western blot analysis of various lysates using SEC61B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-37978_rabbit-sec61b-pab-91220241418191.jpg "Western Blot: SEC61B Antibody [NBP3-37978] - Western blot analysis of various lysates using SEC61B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: SEC61B Antibody [NBP3-37978] - Immunofluorescence analysis of U2OS cells using SEC61B Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37978_rabbit-sec61b-pab-912202415133451.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC61B Antibody [NBP3-37978] - Immunofluorescence analysis of NIH/3T3 cells using SEC61B Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37978_rabbit-sec61b-pab-912202414163041.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC61B Antibody [NBP3-37978] - Immunofluorescence analysis of PC-12 cells using SEC61B Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37978_rabbit-sec61b-pab-912202414154397.jpg)
![Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-immunohistochemistry-1812026169532.jpg "Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]")
![Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-immunohistochemistry-18120261618483.jpg)
![Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-immunohistochemistry-18120261615210.jpg)
![Western Blot:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-western-blot-181202616192816.jpg)
![Western Blot: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (NBP2-12877). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NBP2-12877) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-2G5-G3-Western-Blot-NBP2-12877-img0009.jpg "Western Blot: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (NBP2-12877). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NBP2-12877) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-2G5-G3-Immunocytochemistry-Immunofluorescence-NBP2-12877-img0007.jpg)
![Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Western-Blot-NBP2-12876-img0007.jpg "Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Hsp40 HDJ1 visulaized using, tested on a human keratinocyte line HaCat. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Immunohistochemistry-Paraffin-NBP2-12876-img0004.jpg)
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0003.jpg "Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate")
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Western blot analysis of Dynamin, mAb (D5): Lane 1: MW marker, Lane 2: Mouse Brain, Lane 3: Rat Brain](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0001.jpg)
![Western Blot: Hsp104 Antibody [NBP1-97473] - Analysis of HSP104 (yeast), pAb: Lane 1: MW Marker, Lane 2: Yeast Cell Lysate.](http://images.novusbio.com/fullsize/Hsp104-Antibody-Western-Blot-NBP1-97473-img0001.jpg "Western Blot: Hsp104 Antibody [NBP1-97473] - Analysis of HSP104 (yeast), pAb: Lane 1: MW Marker, Lane 2: Yeast Cell Lysate.")
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-BF-06-Immunofluorescence-NBP1-45050-img0002.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).")
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-BF-06-Immunofluorescence-NBP1-45050-img0001.jpg)
![Western Blot: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4G9-BSA-Free-Western-Blot-NBP1-39850-img0003.jpg "Western Blot: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9")
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4G9-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-39850-img0004.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Analysis of anti-VCP antibody with human prostate at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4G9-BSA-Free-Immunohistochemistry-Paraffin-NBP1-39850-img0001.jpg)
![Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Flow-Cytometry-NBP2-90208-img0003.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t")
![<b>Biological Strategies Validation. </b>Western Blot: ERp72 Antibody (020) [NBP2-90208] - Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Western-Blot-NBP2-90208-img0006.jpg)
![Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208] - PDIA4 was immunoprecipitated using: Lane A :0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. 2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Immunoprecipitation-NBP2-90208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERp72 Antibody (020) [NBP2-90208] - Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90208 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90208_rabbit-monoclonal-erp72-antibody-020-235202511545653.png)
![Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-278202415212484.jpg "Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-298202414333651.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunocytochemistry analysis of HeLa cells labeling HSP90 beta with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-2920241048485.jpg)