| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99331-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26400-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26405-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21903-50ul | |||
![Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Elk-1-Antibody-10U8X1-Western-Blot-NBP3-15631-img0001.jpg "Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Elk-1 antibody (NBP3-15631). Western blot was performed from the immunoprecipitate using Elk-1 antibody (NBP3-15631) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Elk-1-Antibody-10U8X1-Immunoprecipitation-NBP3-15631-img0002.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0003.jpg "Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331] - Immunofluorescence staining of MKK4/MEK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99331 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99331_rabbit-polyclonal-mkk4-mek4-antibody-23520252145179.png)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-282202411363013.jpg "Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 62 KDa")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Staining of A549 cells with AMPK alpha 2 [p Thr172] Antibody (4F4) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-28220241323610.jpg)
![Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Positive Western Blot detected in A549 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF). All lanes: TOR/mTOR [p Ser2481] Antibody at 1.33ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26405_rabbit-tor-mtor-p-ser-2481-mab-3h11-28220241141360.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Positive Western Blot detected in A549 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF). All lanes: TOR/mTOR [p Ser2481] Antibody at 1.33ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa")
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Staining of Hela cells with TOR/mTOR [p Ser2481] Antibody (3H11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26405_rabbit-tor-mtor-p-ser-2481-mab-3h11-282202412475139.jpg)
![Western Blot: DMAP1 Antibody (SR1934) [NBP3-21903] - Western blot analysis using NBP3-21903 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-21903_rabbit-dmap1-mab-sr1934-6720239533712.jpg "Western Blot: DMAP1 Antibody (SR1934) [NBP3-21903] - Western blot analysis using NBP3-21903 in Hela cell lysate.")
![Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.](http://images.novusbio.com/fullsize/AMPK-alpha-1-p-Ser496-Antibody-S01-3B7-Western-Blot-NBP3-19825-img0002.jpg "Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Immunocytochemistry of AMPK alpha 1 (phospho-Ser496) (green) in hela using NBP3-19825 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/AMPK-alpha-1-p-Ser496-Antibody-S01-3B7-Immunocytochemistry-Immunofluorescence-NBP3-19825-img0001.jpg)
![Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.](http://images.novusbio.com/fullsize/MEK1-[p-Ser218-p-Ser222]-Antibody-Western-Blot-NB500-139-img0001.jpg "Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.")
![Recombinant Human TOR/mTOR Protein [H00002475-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002475-Q01-1.jpg "Recombinant Human TOR/mTOR Protein [H00002475-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Proximity Ligation Assay: Elk-1 Antibody (2G6) [H00002002-M01] - Analysis of protein-protein interactions between MAPK3 and ELK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-ELK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Elk-1-Antibody-2G6-Proximity-Ligation-Assay-H00002002-M01-img0002.jpg "Proximity Ligation Assay: Elk-1 Antibody (2G6) [H00002002-M01] - Analysis of protein-protein interactions between MAPK3 and ELK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-ELK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: Elk-1 Antibody (2G6) [H00002002-M01] - Detection limit for recombinant GST tagged ELK1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Elk-1-Antibody-2G6-Sandwich-ELISA-H00002002-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MAPKAPK2 Antibody (1F9) [H00009261-M06] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-1F9-Immunocytochemistry-Immunofluorescence-H00009261-M06-img0002.jpg "Immunocytochemistry/Immunofluorescence: MAPKAPK2 Antibody (1F9) [H00009261-M06] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunoprecipitation: MAPKAPK2 Antibody (1F9) [H00009261-M06] - Analysis of MAPKAPK2 transfected lysate using anti-MAPKAPK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK2 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MAPKAPK2-Antibody-1F9-Immunoprecipitation-H00009261-M06-img0003.jpg)
![Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CDC25A-Antibody-3D5-Proximity-Ligation-Assay-H00000993-M01-img0002.jpg "Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: CDC25A Antibody (3D5) [H00000993-M01] - Detection limit for recombinant GST tagged CDC25A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC25A-Antibody-3D5-Sandwich-ELISA-H00000993-M01-img0003.jpg)