| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54708-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54687-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54690-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44066 | |||
| BSA Free | NBP2-59781-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44492 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44493 | |||
| BSA Free | NBP2-59025-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-12179-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43961 | |||
| BSA Free | NBP2-59777-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1271V | |||
| FITC | NB21-1271F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1271-0.025mg | |||
![Western Blot: alpha Tubulin Antibody [NBP2-54708] - Analysis NBP2-54708 (A) shows similar pattern to independent antibody NBP2-54687 (B). Alpha tubulin molecular weight: 50 kDa).](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54708-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha Tubulin Antibody [NBP2-54708] - Analysis NBP2-54708 (A) shows similar pattern to independent antibody NBP2-54687 (B). Alpha tubulin molecular weight: 50 kDa).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody [NBP2-54708] - Staining of human cell line U-2 OS shows localization to microtubules.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54708-img0005.jpg)
![Western Blot: alpha Tubulin Antibody [NBP2-54708] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54708-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-2032025122428..png)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] -Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-2032025122524..png)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] - Staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-2032025122614..png)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-203202512274..png)
![Western Blot: alpha Tubulin Antibody [NBP2-54687] - Analysis NBP2-54687 (A) shows similar pattern to independent antibody NBP2-54708 (B). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54687-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha Tubulin Antibody [NBP2-54687] - Analysis NBP2-54687 (A) shows similar pattern to independent antibody NBP2-54708 (B). Alpha tubulin molecular weight: 50 kDa.")
![<b>Independent Antibodies Validation. </b>Western Blot: alpha Tubulin Antibody [NBP2-54687] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54687-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody [NBP2-54687] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54687-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0008.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54687] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunohistochemistry-Paraffin-NBP2-54687-img0010.jpg)
![Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NBP2-54690-img0009.jpg "Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human stomach shows moderate membranous positivity.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-54690-img0007.jpg)
![Western Blot: beta-Actin Antibody [NBP2-54690] - Analysis in human cell line U-138MG.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NBP2-54690-img0008.jpg)
![Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human fallopian tube shows moderate positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-54690-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human tonsil shows strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-54690-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Actin Antibody [NBP2-54690] - Staining of human prostate shows moderate positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-54690-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PPIB monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Western-Blot-NBP2-59781-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0014.jpg)
![Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Western-Blot-NBP2-59781-img0010.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0012.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0013.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0015.jpg)
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Theoretical molecular weight: 36 kDa. Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-GAPDH monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GAPDH Antibody (CL3266) [NBP2-59779] - Staining of A549 cells using the Anti-GAPDH monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunocytochemistry-Immunofluorescence-NBP2-59779-img0013.jpg)
![Western Blot: GAPDH Antibody (CL3266) [NBP2-59779] - GAPDH Antibody (2597) [NBP2-59779] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Western-Blot-NBP2-59779-img0008.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human kidney shows cytoplasmic and nuclear immunoreactivity in renal tubules and glomerular cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human fallopian tube shows cytoplasmic and nuclear positivity in the epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human liver shows cytoplasmic and nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0004.jpg)
![Immunohistochemistry: GAPDH Antibody (CL3266) [NBP2-59779] - Human pancreas shows nuclear and cytoplasmic immunoreactivity in both endocrine and exocrine cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3266-Immunohistochemistry-NBP2-59779-img0005.jpg)
![Western Blot: GAPDH Antibody (CL3265) [NBP2-59025] - Theoretical molecular weight: 36 kDa. Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Western-Blot-NBP2-59025-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody (CL3265) [NBP2-59025] - Theoretical molecular weight: 36 kDa. Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human skeletal muscle shows moderate cytoplasmic and strong nuclear immunoreactivity in muscle fibers.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0005.jpg)
![Western Blot: GAPDH Antibody (CL3265) [NBP2-59025] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Western-Blot-NBP2-59025-img0006.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human cerebral cortex shows immunoreactivity in neuropil as well as strong cytoplasmic positivity in a subset of cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0001.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in renal tubules and glomeruli.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0002.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0003.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (CL3265) [NBP2-59025] - Staining of human liver shows strong cytoplasmic and nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-CL3265-Immunohistochemistry-Paraffin-NBP2-59025-img0004.jpg)
![Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Western-Blot-NBP2-54617-img0003.jpg "Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K9/14ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - HeLa cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9/14ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Immunofluorescence-NBP2-54617-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - ChIP assays were performed using human HeLa cells, the antibody against H3K9/14ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-ac-Lys14-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54617-img0001.jpg)
![ELISA: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K9/14ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-ELISA-NBP2-54617-img0002.jpg)
![Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - To test the cross reactivity of the antibody against H3K9/14ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Dot-Blot-NBP2-54617-img0006.jpg)
![Protein Array: Histone H3 [ac Lys9, ac Lys14] Antibody [NBP2-54617] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9--ac-Lys14]-Antibody-Protein-Array-NBP2-54617-img0005.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys9-Antibody-AH3-120-Western-Blot-NB120-12179-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody (AH3-120) [NB120-12179] - Histone fraction isolated from HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) Clone: AH3-120. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Observed molecular weight is ~16 kDa")
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human liver shows cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0005.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2 and mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0006.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0002.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human prostate shows cytoplasmic positivity in the epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-59199-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - ChIP assays were performed using HeLa cells, the antibody against H3K36me3 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59199-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me3 and the crude serum. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:19,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-59199-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K36me3 with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-59199-img0005.jpg)
![Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Western-Blot-NBP3-15393-img0004.jpg "Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded mouse testis using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded rat spleen using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded human colon using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-5H8V8-Immunohistochemistry-Paraffin-NBP3-15393-img0002.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Rat liver using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517202977.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-522025171890.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517284935.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human colon using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517202913.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517223953.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517202933.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517152732.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using Lamin B1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517172857.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517273578.jpg)
![Immunohistochemistry: Lamin B1 Antibody (5H8V8) [Lamin B1] - Immunohistochemistry analysis of paraffin-embedded Human brain using Lamin B1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15393_rabbit-lamin-b1-mab-5h8v8-52202517251911.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Dot-Blot-NB21-1271-img0006.jpg "Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - WB analysis of Histone H3 K37me1 in 1. HeLa histone prep, 2. NIH 3T3 histone prep and 3. C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Western-Blot-NB21-1271-img0007.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Western-Blot-NB21-1271-img0005.jpg)