| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP3-18536 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14062 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55503-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15566-20ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96990 | |||
| FITC | NBP1-96856 | |||
| HRP | NBP1-97069 | |||
| Rhodamine | NBP1-96857 | |||
| Texas Red | NBP1-96985 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25989 | |||
![Other Histone H3 [Monomethyl Lys9] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys9]-Peptide-In-vitro-assay-NB21-1071PEP-img0002.jpg "Other Histone H3 [Monomethyl Lys9] Antibody Blocking Peptide")
![Western Blot: alpha Tubulin Antibody (RM113) [Biotin] [NBP2-77401] - Western blot of A431 cells, using NBP3-18536 at 1/1000 dilution, showed a band of alpha Tubulin at the predicted MW (~50 kDa).](http://images.novusbio.com/fullsize/nbp2-77401_rabbit-monoclonal-alpha-tubulin-antibody-rm113-western-blot-18920252347485.jpg "Western Blot: alpha Tubulin Antibody (RM113) [Biotin] [NBP2-77401] - Western blot of A431 cells, using NBP3-18536 at 1/1000 dilution, showed a band of alpha Tubulin at the predicted MW (~50 kDa).")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (RM113) [NBP2-77401] - Staining of HeLa cells, using NBP2-77401 (red). Nuclei have been labeled with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-77401_rabbit-monoclonal-alpha-tubulin-antibody-rm113-immunocytochemistry-immunofluorescence-199202502436.png)
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Immunocytochemistry-Immunofluorescence-NBP3-13936-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Flow-Cytometry-NBP3-13936-img0001.jpg)
![Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1 Antibody (PCRP-HDAC1-1B7).](http://images.novusbio.com/fullsize/HDAC1-Antibody-PCRP-HDAC1-1B7-Protein-Array-NBP3-13936-img0004.jpg)
![Western Blot: HSP60 Antibody [NBP2-55503] - Analysis using Anti-HSPD1 antibody NBP2-55503 (A) shows similar pattern to independent antibody NBP1-89730 (B).](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-55503-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HSP60 Antibody [NBP2-55503] - Analysis using Anti-HSPD1 antibody NBP2-55503 (A) shows similar pattern to independent antibody NBP1-89730 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP2-55503] - Staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55503-img0001.jpg)
![Western Blot: GAPDH Antibody [NBP2-56153] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-56153-img0002.jpg "Western Blot: GAPDH Antibody [NBP2-56153] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NBP2-56153] - Staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56153-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GAPDH Antibody [NBP2-56153] - Analysis using Anti-GAPDH antibody NBP2-56153 (A) shows similar pattern to independent antibody NBP2-48721 (B). Theoretical molecular weight: 36 kDa](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-56153-img0003.jpg)
![Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Western-Blot-NBP3-15566-img0002.jpg "Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2W4I5-Chromatin-Immunoprecipitation-NBP3-15566-img0003.jpg)
![Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Western-Blot-NBP2-59210-img0001.jpg "Western Blot: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59210-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys79] Antibody [NBP2-59210] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys79]-Antibody-ELISA-NBP2-59210-img0002.jpg)
![Western Blot: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23323_rabbit-beta-actin-p-tyr53-pab-199202312403723.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-Actin [p Tyr53] Antibody [NBP3-23323] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23323_rabbit-beta-actin-p-tyr53-pab-19920231911470.jpg)
![SDS-Page: Transferrin Native Protein [NBC1-04256] - Lane 1: Human Transferrin - Reduced. Lane 2: Human Transferrin - Non-Reduced. Load: 1.0 ug per lane. Observed/Predicted Size: ~70 kDa for Reduced, ~80 kDa for Non-Reduced Transferrin.](http://images.novusbio.com/fullsize/Transferrin-Native-Protein-SDS-Page-NBC1-04256-img0002.jpg "SDS-Page: Transferrin Native Protein [NBC1-04256] - Lane 1: Human Transferrin - Reduced. Lane 2: Human Transferrin - Non-Reduced. Load: 1.0 ug per lane. Observed/Predicted Size: ~70 kDa for Reduced, ~80 kDa for Non-Reduced Transferrin.")
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-RM155-Western-Blot-NBP2-61530-img0004.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.")
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP2-61530] - RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp2-61530_rabbit-monoclonal-histone-h3-trimethyl-lys36-antibody-rm155-multiplex-immunoassay-1892025233710.png)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP2-61530] - ChIP performed on HeLa cells using RM155, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp2-61530_rabbit-monoclonal-histone-h3-trimethyl-lys36-antibody-rm155-chromatin-immunoprecipitation-189202523444712.png)
![Western Blot: Histone H3 [ac Lys36] Antibody (RM154) [NBP2-61541] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using NBP2-61541, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys36-Antibody-RM154-Western-Blot-NBP2-61541-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys36] Antibody (RM154) [NBP2-61541] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using NBP2-61541, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa. Observed molecular weight is ~17 kDa")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys26] Antibody (RM154) - Azide and BSA Free [NBP2-61541] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61541_rabbit-monoclonal-histone-h3-ac-lys36-antibody-rm154-immunocytochemistry-immunofluorescence-189202523394320.png)