| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1271V | |||
| FITC | NB21-1271F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1271-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43847 | |||
| BSA Free | NBP2-61620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48721-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56595SS | |||
| Azide and BSA Free | NBP2-33237 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1095SS | |||
![Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Dot-Blot-NB21-1271-img0006.jpg "Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - WB analysis of Histone H3 K37me1 in 1. HeLa histone prep, 2. NIH 3T3 histone prep and 3. C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Western-Blot-NB21-1271-img0007.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys37] Antibody [NB21-1271] - Dot blot analysis of Histone H3 K37me1 using the peptides listed above in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys37]-Antibody-Western-Blot-NB21-1271-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Immunohistochemistry staining of HepG2 cells using Clone RM166.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-24620241332474.png "Immunohistochemistry-Paraffin: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Immunohistochemistry staining of HepG2 cells using Clone RM166.")
![Western Blot: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM166 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-22620242311027.png)
![Multiplex Immunoassay: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-22620242311050.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM166 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-22620242311068.png)
![Sandwich ELISA: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - Sandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using an anti-Histone H3 (1 ug/mL) as the capture antibody and biotinylated clone RM166 (2 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-2462024132632.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody (RM166) - Azide and BSA Free [NBP3-25995] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using Clone RM166 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25995_rabbit-histone-h3-mab-rm166-azide-and-bsa-free-chromatin-immunoprecipitation-2462024225351..png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-24620241332436.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Immunohistochemistry staining of HepG2 cells using Clone RM161.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-226202422321216.png)
![Multiplex Immunoassay: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-226202422321241.png)
![Western Blot: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with Sodium Butyrate, using RM161 at 0.25 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-2462024133247.png)
![Sandwich ELISA: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - Sandwich ELISA against acetylated histone H3 at Lys 9 using Clone RM161 (1 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-24620241332451.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody (RM161) - Azide and BSA Free [NBP3-25994] - ChIP performed on HeLa cells using Clone RM161 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25994_rabbit-histone-h3-mab-rm161-azide-and-bsa-free-22620242311074.png)
![Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Western-Blot-NBP2-61620-img0006.jpg "Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620] - Staining of MCF7 cells using the Anti-COX4I1 monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunocytochemistry-Immunofluorescence-NBP2-61620-img0007.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human skin shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-Paraffin-NBP2-61620-img0005.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0002.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0003.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0004.jpg)
![Western Blot: GAPDH Antibody [NBP2-48721] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB. GAPDH theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NBP2-48721-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GAPDH Antibody [NBP2-48721] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB. GAPDH theoretical molecular weight: 36 kDa.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NBP2-48721] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48721-img0005.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NBP2-48721] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NBP2-48721-img0012.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NBP2-48721] - Staining of human gastrointestinal shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NBP2-48721-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NBP2-48721] - Staining of human prostate shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NBP2-48721-img0010.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NBP2-48721] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NBP2-48721-img0011.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Western Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K20me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~11 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-54620-img0005.jpg "Western Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K20me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~11 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - HeLa cells were stained with the antibody against H4K20me3 and with DAPI. Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Immunofluorescence-NBP2-54620-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - ChIP assays were performed using human K562 cells, the antibody against H4K20me3 and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoters of the active genes EIF4A2 and GAPDH, used as negative controls, and for ZNF510 and the Sat2 satellite repeat region used as positive controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys20-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54620-img0001.jpg)
![ELISA: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K20me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,700.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-54620-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - To test the cross reactivity of the antibody against H4K20me3. A Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-54620-img0003.jpg)
![Protein Array: Histone H3 [Trimethyl Lys20] Antibody [NBP2-54620] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys20]-Antibody-Protein-Array-NBP2-54620-img0004.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Western-Blot-NBP2-54613-img0004.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - HeLa cells were stained with the antibody against H3K4me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Immunofluorescence-NBP2-54613-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - ChIP assays were performed using human K562 cells, the antibody against H3K4me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-3-Monomethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54613-img0008.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - To test the cross reactivity of the antibody against H3K4me1, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Dot-Blot-NBP2-54613-img0003.jpg)
![ELISA: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K4me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:10,300.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-ELISA-NBP2-54613-img0009.jpg)
![Protein Array: Histone H3 [Monomethyl Lys4] Antibody [NBP2-54613] - Histone 3 [Monomethyl Lys4] Antibody [NBP2-54613] - The specificity of the antibody was further demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:2,000. Figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.](http://images.novusbio.com/fullsize/Histone-3-[Monomethyl-Lys4]-Antibody-Protein-Array-NBP2-54613-img0010.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-117C826-Western-Blot-NB100-56595-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Ser28] Antibody (117C826) [NB100-56595] - Analysis using Azide and BSA Free version of NB100-56595. Histone H3 phospho (Ser28) in (1) untreated, and (2) Fas antibody treated Jurkat lysate using Histone H3 phospho antibody at 2 ug/ml. Observed molecular weight is ~17 kDa")
![Western Blot: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Knockout-Validated-NBP2-24770-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody [NBP2-24770] - analysis of Cyclophilin B in A) HeLa cell lysate and B) 293 cell lysate using this antibody. 5 and 2 ug/ml, respectively. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-24770-img0001.jpg)
![Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.](http://images.novusbio.com/fullsize/antibody/nb21-1095_rabbit-histone-h3-p-ser10-ac-lys9-pab-western-blot-1132024114213.jpg "Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.")
![Dot Blot analysis of Histone H3 [p Ser10, ac Lys9] Antibody in the peptides listed in 1.0 and 0.5 picomoles of peptide.](http://images.novusbio.com/fullsize/antibody/nb21-1095_rabbit-histone-h3-p-ser10-ac-lys9-pab-dot-blot-1132024115254..jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NBP2-59187-img0003.jpg "Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K4me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Theoretical molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Panel B, C, D and E: staining of the cells with the H3K4me2 antibody after incubation of the antibody with 5 ng/uL blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K4, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K4me2 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. A: cells were immunofluorescently labeled with the H3K4me2 antibody (left) diluted 1:5,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59187-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - ChIP was performed with the antibody against H3K4me2 on sheared chromatin from 500,000 HeLaS3 cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration of the antibody of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59187-img0006.jpg)
![ELISA: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H3K4me2 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:48,200.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-ELISA-NBP2-59187-img0001.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NBP2-59187] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me2 with peptides containing other modifications of histone H3 and H4 and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59187-img0002.jpg)