| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31925-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP3-49664AFP405 | |||
| Alexa Fluor Plus 488 | NBP3-49664AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49664AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49664AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49664AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49664AFP680 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NB600-931AFP405 | |||
| Alexa Fluor Plus 488 | NB600-931AFP488 | |||
| Alexa Fluor Plus 555 | NB600-931AFP555 | |||
| Alexa Fluor Plus 594 | NB600-931AFP594 | |||
| Alexa Fluor Plus 647 | NB600-931AFP647 | |||
| Alexa Fluor Plus 680 | NB600-931AFP680 | |||
![Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31925-img0010.jpg "Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Immunohistochemistry-Paraffin-NBP2-31925-img0008.jpg)
![Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Western-Blot-NBP2-59217-img0001.jpg "Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - ChIP assays were performed using human HeLa cells, treated with TSA, the antibody against H3K18ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NBP2-59217-img0003.jpg)
![ELISA: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K18ac . The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-ELISA-NBP2-59217-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - HeLa cells were stained with the antibody against H3K18ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18-Dimethyl-Arg17]-Antibody-Immunofluorescence-NBP2-59217-img0004.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Western-Blot-NBP2-59294-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Immunofluorescence-NBP2-59294-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K9me3 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 3 and 9 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Chromatin-Immunoprecipitation-NBP2-59294-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Cross reactivity of the antibody directed against H3K9me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K9me2. The wells were coated with peptides containing the unmodified H3K9 as well as the mono-, di- and trimethylated H3K9 and the dimethylated H3K4 and H3K27. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-ELISA-NBP2-59294-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Western-Blot-NBP2-59295-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Immunofluorescence-NBP2-59295-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me3 and optimized PCR primer sets for qPCR. ChIP was performed with the AutoHistone ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Chromatin-Immunoprecipitation-NBP2-59295-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Cross reactivity of the antibody directed against H3K27me3. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K27me3. The wells were coated with peptides containing the unmodified H3K27 region as well as the mono-, di- and trimethylated H3K27 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-ELISA-NBP2-59295-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - ChIP assays were performed using human HeLa cells treated with colcemid, the antibody against H3K27me3S28p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active gene GAPDH as a negative control, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3S28p is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27-p-Ser28]-Antibody-Chromatin-Immunoprecipitation-NBP2-59212-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Western blot was performed on histone extracts (15 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 2) using the antibody against H3K27me3S28p diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0005.jpg)
![ELISA: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27me3S28p. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-ELISA-NBP2-59212-img0001.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me3S28p with peptides containing other modifications of histone H3 and H4 and the unmodified H3K27S28 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:2,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Dot-Blot-NBP2-59212-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Immunofluorescence-NBP2-59301-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Chromatin-Immunoprecipitation-NBP2-59301-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me3. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-ELISA-NBP2-59301-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-119202316331610.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NBP3-23319] - Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).")
![Western Blot: beta-Actin Antibody [NBP3-23319] - Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23319_rabbit-beta-actin-pab-19920231915417.jpg)
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-20720231348119.jpg%3Fv=1681426096 "Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).")
![Western Blot: beta-Actin Antibody (M202) [NBP3-23331] - Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).](http://images.novusbio.com/fullsize/nbp3-23331_mouse-beta-actin-mab-m202-19920231410116.jpg)
![Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: Rabbit Antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 649 conjugated (green), Goat IgG (H&L) Antibody DyLight (TM) 549 conjugated (red), Mouse IgG (H&L) Antibody DyLight (TM) 488 conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer for 1hr at 4C.](http://images.novusbio.com/fullsize/Transferrin Antibody-Western Blot-NB600-931-img0004.jpg "Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: Rabbit Antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 649 conjugated (green), Goat IgG (H&L) Antibody DyLight (TM) 549 conjugated (red), Mouse IgG (H&L) Antibody DyLight (TM) 488 conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer for 1hr at 4C.")
![Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NB600-931-img0002.jpg)
![Western Blot: Transferrin Antibody [NB600-931] - Lane 1: Human Transferrin. Lane 2: None.](http://images.novusbio.com/fullsize/Transferrin-Antibody-Western-Blot-NB600-931-img0003.jpg)
