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![Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.](http://images.novusbio.com/fullsize/Recombinant-Rat-Adiponectin-Acrp30-FLAG-Protein-Western-Blot-NBP3-18139-img0001.jpg "Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.")
![Western Blot: Recombinant Rat Progranulin/PGRN FLAG Protein [NBP3-18147] - To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.](http://images.novusbio.com/fullsize/Recombinant-Rat-Progranulin-PGRN-FLAG-Protein-Western-Blot-NBP3-18147-img0001.jpg "Western Blot: Recombinant Rat Progranulin/PGRN FLAG Protein [NBP3-18147] - To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.")
![Western Blot: Recombinant Rat Progranulin/PGRN Protein [NBP3-18148] - Deglycosylation of rat progranulin. To examine the deglycosylation of rat progranulin, 1ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37C. Separation of reaction products is visualized by immunoblotting using anti-progranulin (rat) antibody.](http://images.novusbio.com/fullsize/Recombinant-Rat-Progranulin-PGRN-Protein-Western-Blot-NBP3-18148-img0001.jpg "Western Blot: Recombinant Rat Progranulin/PGRN Protein [NBP3-18148] - Deglycosylation of rat progranulin. To examine the deglycosylation of rat progranulin, 1ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37C. Separation of reaction products is visualized by immunoblotting using anti-progranulin (rat) antibody.")
![Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-E981-Western-Blot-NBP3-18297-img0001.jpg "Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.")
![Western Blot: 12-Lipoxygenase Antibody [H00000239-D01P] - Analysis of ALOX12 expression in transfected 293T cell line by ALOX12 polyclonal antibody.Lane 1: ALOX12 transfected lysate(75.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/12-Lipoxygenase-Antibody-Western-Blot-H00000239-D01P-img0003.jpg "Western Blot: 12-Lipoxygenase Antibody [H00000239-D01P] - Analysis of ALOX12 expression in transfected 293T cell line by ALOX12 polyclonal antibody.Lane 1: ALOX12 transfected lysate(75.70 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: RFK Antibody [H00055312-B01P] - Analysis of RFK expression in transfected 293T cell line by RFK polyclonal antibody. Lane 1: RFK transfected lysate(17.82 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RFK-Antibody-Western-Blot-H00055312-B01P-img0001.jpg "Western Blot: RFK Antibody [H00055312-B01P] - Analysis of RFK expression in transfected 293T cell line by RFK polyclonal antibody. Lane 1: RFK transfected lysate(17.82 KDa). Lane 2: Non-transfected lysate.")
![ELISA: Perilipin Antibody (4G1) [H00005346-M09] - Detection limit for recombinant GST tagged PLIN is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Perilipin-Antibody-4G1-ELISA-H00005346-M09-img0001.jpg "ELISA: Perilipin Antibody (4G1) [H00005346-M09] - Detection limit for recombinant GST tagged PLIN is approximately 3ng/ml as a capture antibody.")
![Western Blot: MAN2B2 Antibody [H00023324-B01P] - Analysis of MAN2B2 expression in transfected 293T cell line by MAN2B2 polyclonal antibody. Lane 1: MAN2B2 transfected lysate(110.99 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MAN2B2-Antibody-Western-Blot-H00023324-B01P-img0002.jpg "Western Blot: MAN2B2 Antibody [H00023324-B01P] - Analysis of MAN2B2 expression in transfected 293T cell line by MAN2B2 polyclonal antibody. Lane 1: MAN2B2 transfected lysate(110.99 KDa). Lane 2: Non-transfected lysate.")
![Sandwich ELISA: SUR1 Antibody (3E1) [H00006833-M02] - Detection limit for recombinant GST tagged ABCC8 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SUR1-Antibody-3E1-Sandwich-ELISA-H00006833-M02-img0001.jpg "Sandwich ELISA: SUR1 Antibody (3E1) [H00006833-M02] - Detection limit for recombinant GST tagged ABCC8 is 0.03 ng/ml as a capture antibody.")
![Western Blot: CAR/NR1I3 Antibody [H00009970-D01P] - Analysis of NR1I3 expression in transfected 293T cell line by NR1I3 polyclonal antibody.Lane 1: NR1I3 transfected lysate(39.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-Western-Blot-H00009970-D01P-img0002.jpg "Western Blot: CAR/NR1I3 Antibody [H00009970-D01P] - Analysis of NR1I3 expression in transfected 293T cell line by NR1I3 polyclonal antibody.Lane 1: NR1I3 transfected lysate(39.90 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Mannose Phosphate Isomerase Antibody [H00004351-B01P] - Analysis of MPI expression in transfected 293T cell line by MPI polyclonal antibody. Lane 1: MPI transfected lysate(46.53 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-Western-Blot-H00004351-B01P-img0002.jpg "Western Blot: Mannose Phosphate Isomerase Antibody [H00004351-B01P] - Analysis of MPI expression in transfected 293T cell line by MPI polyclonal antibody. Lane 1: MPI transfected lysate(46.53 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: ENPP4 Antibody [H00022875-B01P] - Analysis of ENPP4 expression in transfected 293T cell line by ENPP4 polyclonal antibody. Lane 1: ENPP4 transfected lysate(49.83 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ENPP4-Antibody-Western-Blot-H00022875-B01P-img0001.jpg "Western Blot: ENPP4 Antibody [H00022875-B01P] - Analysis of ENPP4 expression in transfected 293T cell line by ENPP4 polyclonal antibody. Lane 1: ENPP4 transfected lysate(49.83 KDa). Lane 2: Non-transfected lysate.")