| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38382-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02995-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89207-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98977-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97369-50ul | |||
![Immunocytochemistry/ Immunofluorescence: ARH Antibody [NBP3-38382] - Immunofluorescence analysis of A-549 cells using ARH Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-38382_rabbit-arh-pab-912202415171191.jpg "Immunocytochemistry/ Immunofluorescence: ARH Antibody [NBP3-38382] - Immunofluorescence analysis of A-549 cells using ARH Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.")
![Western Blot: ARH Antibody [NBP3-38382] - Western blot analysis of various lysates using ARH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38382_rabbit-arh-pab-912202415133429.jpg)
![Western Blot: 4EBP1 Antibody [NBP3-02995] - Analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 300s.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP3-02995-img0004.jpg "Western Blot: 4EBP1 Antibody [NBP3-02995] - Analysis of extracts of various cell lines, using eIF4EBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 300s.")
![Western Blot: ATF6 Antibody (8D3-R) [NBP3-32054] - Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (NBP3-32054) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32054_mouse-atf6-mab-8d3-r-278202416331270.jpg "Western Blot: ATF6 Antibody (8D3-R) [NBP3-32054] - Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (NBP3-32054) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32054) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ATF6 Antibody (8D3-R) [NBP3-32054] - Immunocytochemistry analysis of HeLa cells labeling ATF6 with Mouse anti-ATF6 antibody (NBP3-32054) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ATF6 antibody (NBP3-32054) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32054_mouse-atf6-mab-8d3-r-29202410234226.jpg)
![Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-278202416285235.jpg "Western Blot: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Western blot analysis of FABP3/H-FABP on different lysates with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FABP3/H-FABP Antibody (JE53-37) [NBP3-32325] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3/H-FABP antibody (NBP3-32325) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32325) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32325_rabbit-fabp3-h-fabp-mab-je53-37-29820241435241.jpg)
![Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89207_rabbit-monoclonal-4ebp1-antibody-007-23520251154563.png "Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89207 (1:60) at 37 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.")
![Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-278202415212425.jpg "Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Immunocytochemistry analysis of NIH/3T3 cells starved overnight then treated with or without 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes labeling TBC1D4 with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-29202410484887.jpg)
![Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-288202412461622.jpg "Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (JE39-00) [NBP3-32833] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32833) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-298202414304140.jpg)
![Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP2-98977-img0003.jpg "Western Blot: ABCF2 Antibody [NBP2-98977] - Anti-ABCF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa")
![Immunoprecipitation: ABCF2 Antibody [NBP2-98977] - ABCF2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 1 ul anti-ABCF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ABCF2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunoprecipitation-NBP2-98977-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF2 Antibody [NBP2-98977] - Immunofluorescence staining of ABCF2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98977 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98977_rabbit-polyclonal-abcf2-antibody-23520251125448.png)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-97369-img0002.jpg "Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Derlin 1 Antibody - BSA Free [NBP2-97369] - Immunochemical staining of human Derlin 1 in human brain with NBP2-97369 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-97369_rabbit-polyclonal-derlin-1-antibody-235202514492372.png)
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP2-97369] - Immunofluorescence staining of Derlin 1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97369 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97369_rabbit-polyclonal-derlin-1-antibody-23520252135188.png)
![Western Blot: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23246_mouse-caveolin-1-p-tyr14-mab-m283-19920231856942.jpg "Western Blot: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23246_mouse-caveolin-1-p-tyr14-mab-m283-199202312523624.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23419_rat-sphingosine-kinase-1-sphk1-mab-m540-199202312523633.jpg "Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23419_rat-sphingosine-kinase-1-sphk1-mab-m540-19920231856961.jpg)