| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47181SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-07075SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54676-25ul | |||
![Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Dimethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69089-img0002.jpg "Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.")
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-9-Antibody-exon-9-Western-Blot-NBP1-47178-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-[exon-13]-Antibody-Western-Blot-NBP1-47181-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.")
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2597C) [NBP3-07075] - HIF-2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Human HIF-2 alpha /EPAS1 Monoclonal Antibody (Catalog # NBP3-07075) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2597C-Immunohistochemistry-Paraffin-NBP3-07075-img0001.jpg "Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2597C) [NBP3-07075] - HIF-2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Human HIF-2 alpha /EPAS1 Monoclonal Antibody (Catalog # NBP3-07075) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Recombinant Human EGLN1/PHD2 GST (N-Term) Protein [H00054583-P01] - Analysis by 12.5% SDS-PAGE. Gel stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00054583-P01-1.jpg "Recombinant Human EGLN1/PHD2 GST (N-Term) Protein [H00054583-P01] - Analysis by 12.5% SDS-PAGE. Gel stained with Coomassie Blue.")
![SDS-Page: Recombinant Human HIF-2 alpha/EPAS1 Protein [H00002034-P03] - 12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/Recombinant-Human-HIF-2-alpha-EPAS1-Protein-SDS-Page-H00002034-P03-img0002.jpg "SDS-Page: Recombinant Human HIF-2 alpha/EPAS1 Protein [H00002034-P03] - 12.5% SDS-PAGE Stained with Coomassie Blue")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NBP2-54676] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54676-img0003.jpg "Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NBP2-54676] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.")
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody [NBP2-54676] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-Immunohistochemistry-Paraffin-NBP2-54676-img0001.jpg)