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![Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.](http://images.novusbio.com/fullsize/Recombinant-Rat-Adiponectin-Acrp30-FLAG-Protein-Western-Blot-NBP3-18139-img0001.jpg "Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.")
![RBP4/Retinol-Binding Protein 4 Antibody [NBP2-80108] - Analysis using RBP4 (mouse), pAb (Prod. No. NBP2-80108) at 1:2'000 dilution.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-NBP2-80108-img0001.jpg "RBP4/Retinol-Binding Protein 4 Antibody [NBP2-80108] - Analysis using RBP4 (mouse), pAb (Prod. No. NBP2-80108) at 1:2'000 dilution.")
![Western Blot: Recombinant Rat Progranulin/PGRN FLAG Protein [NBP3-18147] - To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.](http://images.novusbio.com/fullsize/Recombinant-Rat-Progranulin-PGRN-FLAG-Protein-Western-Blot-NBP3-18147-img0001.jpg "Western Blot: Recombinant Rat Progranulin/PGRN FLAG Protein [NBP3-18147] - To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.")
![Western Blot: Recombinant Rat Progranulin/PGRN Protein [NBP3-18148] - Deglycosylation of rat progranulin. To examine the deglycosylation of rat progranulin, 1ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37C. Separation of reaction products is visualized by immunoblotting using anti-progranulin (rat) antibody.](http://images.novusbio.com/fullsize/Recombinant-Rat-Progranulin-PGRN-Protein-Western-Blot-NBP3-18148-img0001.jpg "Western Blot: Recombinant Rat Progranulin/PGRN Protein [NBP3-18148] - Deglycosylation of rat progranulin. To examine the deglycosylation of rat progranulin, 1ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37C. Separation of reaction products is visualized by immunoblotting using anti-progranulin (rat) antibody.")
![Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-E981-Western-Blot-NBP3-18297-img0001.jpg "Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.")
![Western Blot: PINK1 Overexpression Lysate (Denatured) [H00065018-T02] - Lane 1: PINK1 transfected lysate, obseved molecular weight at 62.70 KDaLane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PINK1-Overexpression-Lysate-Denatured-Western-Blot-H00065018-T02-img0003.jpg "Western Blot: PINK1 Overexpression Lysate (Denatured) [H00065018-T02] - Lane 1: PINK1 transfected lysate, obseved molecular weight at 62.70 KDaLane 2: Non-transfected lysate.")
![ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve](http://images.novusbio.com/fullsize/Leptin-OB-ELISA-Kit-[HRP]-ELISA-KA0026-img0001.jpg "ELISA: Leptin/OB ELISA Kit [HRP] [KA0026] - Standard curve")
![Western Blot: NME6 Antibody [H00010201-B03P] - Analysis of NME6 expression in transfected 293T cell line. Lane 1: NME6 transfected lysate (21.34 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NME6-Antibody-Western-Blot-H00010201-B03P-img0002.jpg "Western Blot: NME6 Antibody [H00010201-B03P] - Analysis of NME6 expression in transfected 293T cell line. Lane 1: NME6 transfected lysate (21.34 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: EDEM2 Antibody [H00055741-D01P] - Analysis of EDEM2 expression in transfected 293T cell line by EDEM2 polyclonal antibody.Lane 1: EDEM2 transfected lysate(64.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EDEM2-Antibody-Western-Blot-H00055741-D01P-img0002.jpg "Western Blot: EDEM2 Antibody [H00055741-D01P] - Analysis of EDEM2 expression in transfected 293T cell line by EDEM2 polyclonal antibody.Lane 1: EDEM2 transfected lysate(64.80 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: IA-2/PTPRN Antibody [H00005798-D01P] - Analysis of PTPRN expression in transfected 293T cell line by PTPRN polyclonal antibody.Lane 1: PTPRN transfected lysate(102.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/IA-2-PTPRN-Antibody-Western-Blot-H00005798-D01P-img0002.jpg "Western Blot: IA-2/PTPRN Antibody [H00005798-D01P] - Analysis of PTPRN expression in transfected 293T cell line by PTPRN polyclonal antibody.Lane 1: PTPRN transfected lysate(102.80 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: PMM2/Phosphomannomutase 2 Antibody (2A5) [H00005373-M02] - PMM2 monoclonal antibody (M02), clone 2A5. Analysis of PMM2 expression in K-562.](http://images.novusbio.com/fullsize/PMM2-Phosphomannomutase-2-Antibody-2A5-Western-Blot-H00005373-M02-img0002.jpg "Western Blot: PMM2/Phosphomannomutase 2 Antibody (2A5) [H00005373-M02] - PMM2 monoclonal antibody (M02), clone 2A5. Analysis of PMM2 expression in K-562.")