| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92271-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35664-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35735-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38266-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90470-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90272-50ul | |||
![Western Blot: Ceramide Kinase Antibody [NBP2-92271] - Analysis of extracts of various cell lines, using Ceramide Kinase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Western-Blot-NBP2-92271-img0001.jpg "Western Blot: Ceramide Kinase Antibody [NBP2-92271] - Analysis of extracts of various cell lines, using Ceramide Kinase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Western Blot: GPD2 Antibody [NBP3-35664] - Western blot analysis of various lysates, using GPD2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35664_rabbit-gpd2-pab-1012202411433448.jpg "Western Blot: GPD2 Antibody [NBP3-35664] - Western blot analysis of various lysates, using GPD2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: TRIM5 alpha Antibody [NBP3-35735] - Western blot analysis of various lysates using TRIM5 alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35735_rabbit-trim5-alpha-pab-1012202411381271.jpg "Western Blot: TRIM5 alpha Antibody [NBP3-35735] - Western blot analysis of various lysates using TRIM5 alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Guanine deaminase Antibody [NBP3-38266] - Western blot analysis of various lysates using Guanine deaminase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38266_rabbit-guanine-deaminase-pab-10122024121011.jpg "Western Blot: Guanine deaminase Antibody [NBP3-38266] - Western blot analysis of various lysates using Guanine deaminase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: PDE8A Antibody (EM-52) [NBP2-62213] - Analysis (reducing conditions) of PDE8a in HEK293T/17-PDE8a transfectants using mouse monoclonal antibody EM-52.](http://images.novusbio.com/fullsize/PDE8A-Antibody-EM-52-Western-Blot-NBP2-62213-img0001.jpg "Western Blot: PDE8A Antibody (EM-52) [NBP2-62213] - Analysis (reducing conditions) of PDE8a in HEK293T/17-PDE8a transfectants using mouse monoclonal antibody EM-52.")
![Immunohistochemistry-Paraffin: CD38 Antibody (32) [NBP2-90470] - Staining of mouse CD38 in mouse kidney with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.](http://images.novusbio.com/fullsize/CD38-Antibody-32-Immunohistochemistry-Paraffin-NBP2-90470-img0002.jpg "Immunohistochemistry-Paraffin: CD38 Antibody (32) [NBP2-90470] - Staining of mouse CD38 in mouse kidney with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.")
![Immunohistochemistry-Paraffin: CD38 Antibody (32) [NBP2-90470] - Staining of mouse CD38 in mouse heart with rabbit monoclonal antibody (1:500, formalin-fixed paraffin embedded sections). Positive staining was localized to endotheliocyte.](http://images.novusbio.com/fullsize/CD38-Antibody-32-Immunohistochemistry-Paraffin-NBP2-90470-img0001.jpg)
![Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32790_rabbit-pras40-p-thr246-mab-je53-94-288202412442948.jpg "Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Western-Blot-NBP2-90272-img0001.jpg "Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa")
![Immunoprecipitation: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Immunoprecipitation-NBP2-90272-img0002.jpg)
![Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32402_rabbit-gpd2-mab-psh0-31-278202416331284.png "Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: GPD2 Antibody (PSH0-31) [NBP3-32402] - Flow cytometric analysis of Hela cells labeling GPD2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32402, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32402_rabbit-gpd2-mab-psh0-31-392024884666.png)
![Immunohistochemistry: Ferritin Heavy Chain Antibody [NBP3-09324] - Immunohistochemical analysis of kidney.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Immunohistochemistry-NBP3-09324-img0001.jpg "Immunohistochemistry: Ferritin Heavy Chain Antibody [NBP3-09324] - Immunohistochemical analysis of kidney.")