| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95146-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89460-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23518-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26589-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26154-50ul | |||
![Western Blot: xCT Antibody - Azide and BSA Free [xCT] - Western blot analysis of lysates from Rat brain using xCT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-95146_rabbit-polyclonal-xct-antibody-5220251628035.jpg "Western Blot: xCT Antibody - Azide and BSA Free [xCT] - Western blot analysis of lysates from Rat brain using xCT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54) [NBP3-33134] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33134) at 1/1,000 dilution. Lane 1: SH-SY5Y whole cell lysate Lane 2: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: SH-SY5Y treated with pp for 1 hour whole cell lysate Lane 4: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 277 kDa Observed band size: 277 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33134) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33134_rabbit-acetyl-coa-carboxylase-alpha-acaca-p-ser79-mab-28820241423312.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54) [NBP3-33134] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33134) at 1/1,000 dilution. Lane 1: SH-SY5Y whole cell lysate Lane 2: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: SH-SY5Y treated with pp for 1 hour whole cell lysate Lane 4: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 277 kDa Observed band size: 277 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33134) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33135) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes cell lysate Lane 3: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 265 kDa Observed band size: 265 kDa Exposure time: 1 minutes 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33135) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33135_rabbit-acetyl-coa-carboxylase-alpha-acaca-p-ser79-mab-28820241423319.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33135) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes cell lysate Lane 3: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 265 kDa Observed band size: 265 kDa Exposure time: 1 minutes 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33135) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to intestinal gland.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-214-Immunohistochemistry-Paraffin-NBP2-89460-img0002.jpg "Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to intestinal gland.")
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (214) [NBP2-89460] - Staining of human TXN in human colon with rabbit monoclonal antibody (1:200). Positive staining was localized to colonic gland.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-214-Immunohistochemistry-Paraffin-NBP2-89460-img0001.jpg)
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp3-23518_rabbit-4ebp1-mab-0014-azide-and-bsa-free-235202511545617.png "Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with IGF1 (100ng/ml, 15min;+) (line B); treated with IGF1 and -phosphatase (line C) using NBP3-23518 at 1:5000 dilution.")
![Western Blot: 4EBP1 [p Thr46, p Thr37] Antibody (0014) [NBP3-23518] - Western blot analysis of extracts from HEK293, untreated(line A) or treated with IGF1 (100ng/ml, 15min; +)(line B), using NBP3-23518 at 1:5000 dilution (upper) or Mouse Monoclonal 4EBP1 Antibody at 1:5000 dilution (lower).](http://images.novusbio.com/fullsize/nbp3-23518_rabbit-4ebp1-mab-0014-azide-and-bsa-free-23520251628169.png)
![Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/nbp3-26078_mouse-14-3-3-epsilon-mab-02-azide-and-bsa-free-136202422345087.png "Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate 4 uL NBP3-26078 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26078, at 1:100 dilution. Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2 [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26078_mouse-14-3-3-epsilon-mab-02-azide-and-bsa-free-136202422345019.png)
![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (2A5) [NBP3-26589] - Staining of Hela Cells with Fatty Acid Synthase/FASN Antibody (2A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26589_rabbit-fatty-acid-synthase-fasn-mab-2a5-282202412493622.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (2A5) [NBP3-26589] - Staining of Hela Cells with Fatty Acid Synthase/FASN Antibody (2A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Fatty Acid Synthase/FASN Antibody (2A5) [NBP3-26589] - Overlay histogram showing A549 cells stained with Fatty Acid Synthase/FASN Antibody (2A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26589_rabbit-fatty-acid-synthase-fasn-mab-2a5-282202414183634.jpg)
![Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-28220241144268.jpg "Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa")
![Flow Cytometry: LC3B Antibody (7D11) [NBP3-26154] - Overlay Peak curve showing Hela cells stained with LC3B Antibody (7D11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-282202414183621.jpg)