| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58537-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35405-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22666-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94312-0.02ml | |||
![Immunocytochemistry/Immunofluorescence: PFKP Antibody [NBP2-58537] - Staining of human cell line MCF7 shows localization to cytosol.](http://images.novusbio.com/fullsize/PFKP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58537-img0001.jpg "Immunocytochemistry/Immunofluorescence: PFKP Antibody [NBP2-58537] - Staining of human cell line MCF7 shows localization to cytosol.")
![Western Blot: PFKP Antibody [NBP2-58537] -Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/antibody/nbp2-58537_rabbit-polyclonal-pfkp-antibody-western-blot-13112024103415..png)
![Western Blot: PFKP Antibody [NBP2-32192] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFKP antibody NBP2-32192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PFKP-Antibody-Western-Blot-NBP2-32192-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PFKP Antibody [NBP2-32192] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFKP antibody NBP2-32192 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PFKP Antibody [NBP2-32192] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFKP antibody NBP2-32192 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFKP, NBP2-32192 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PFKP-Antibody-Immunoprecipitation-NBP2-32192-img0002.jpg)
![Western Blot: DGK-alpha Antibody [NBP3-35405] - Western blot analysis of various lysates using DGK-alpha Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-35405_rabbit-dgk-alpha-pab-10122024120614.jpg "Western Blot: DGK-alpha Antibody [NBP3-35405] - Western blot analysis of various lysates using DGK-alpha Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Western Blot: DGK-alpha Antibody (5T6O5) [NBP3-33332] - Western blot analysis of various lysates, using DGK-alpha Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33332_rabbit-dgk-alpha-mab-5t6o5-1012202411404049.jpg "Western Blot: DGK-alpha Antibody (5T6O5) [NBP3-33332] - Western blot analysis of various lysates, using DGK-alpha Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: DGK-alpha Antibody (5T6O5) [NBP3-33332] - Western blot analysis of lysates from Jurkat cells, using DGK-alpha Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33332_rabbit-dgk-alpha-mab-5t6o5-101220241136024.jpg)
![Western Blot: DGAT1 Antibody (JE34-88) [NBP3-32265] - Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32265) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32265_rabbit-dgat1-mab-je34-88-278202416331280.jpg "Western Blot: DGAT1 Antibody (JE34-88) [NBP3-32265] - Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32265) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DGAT1 Antibody (JE34-88) [NBP3-32265] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32265) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32265_rabbit-dgat1-mab-je34-88-29820241492691.jpg)
![Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22666_rabbit-dgat1-mab-sr1829-57202316494249.jpg "Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.")
![Western Blot: DGK-alpha Antibody (S06-6H4) [NBP3-19856] - Western blot detection of DGK-alpha in Hela, A549 cell lysates using NBP3-19856 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.](http://images.novusbio.com/fullsize/DGK-alpha-Antibody-S06-6H4-Western-Blot-NBP3-19856-img0002.jpg "Western Blot: DGK-alpha Antibody (S06-6H4) [NBP3-19856] - Western blot detection of DGK-alpha in Hela, A549 cell lysates using NBP3-19856 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.")
![Immunohistochemistry-Paraffin: DGK-alpha Antibody (S06-6H4) [NBP3-19856] - Immunohistochemistry of DGK-alpha in paraffin-embedded Human tonsil using NBP3-19856 at dilution 1/50](http://images.novusbio.com/fullsize/DGK-alpha-Antibody-S06-6H4-Immunohistochemistry-Paraffin-NBP3-19856-img0001.jpg)
![Western Blot: DGK-alpha Antibody [H00001606-D01P] - Analysis of DGKA expression in human placenta.](http://images.novusbio.com/fullsize/DGK-alpha-Antibody-Western-Blot-H00001606-D01P-img0002.jpg "Western Blot: DGK-alpha Antibody [H00001606-D01P] - Analysis of DGKA expression in human placenta.")
![Western Blot: DGK-alpha Antibody [H00001606-D01P] - Analysis of DGKA expression in K-562.](http://images.novusbio.com/fullsize/DGK-alpha-Antibody-Western-Blot-H00001606-D01P-img0001.jpg)
![Western Blot: DGK-alpha Antibody [H00001606-D01P] - Analysis of DGKA expression in transfected 293T cell line by DGKA polyclonal antibody.Lane 1: DGKA transfected lysate(82.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DGK-alpha-Antibody-Western-Blot-H00001606-D01P-img0003.jpg)
![Western Blot: PFKP Overexpression Lysate (Adult Normal) [NBL1-14317] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PFKP.](http://images.novusbio.com/fullsize/PFKP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14317-img0002.jpg "Western Blot: PFKP Overexpression Lysate (Adult Normal) [NBL1-14317] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PFKP.")
![Western Blot: PFKP Overexpression Lysate (Adult Normal) [NBL1-14317] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PFKP](http://images.novusbio.com/fullsize/PFKP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14317-img0001.jpg)
![Western Blot: PLCD4 Antibody [NBP2-94312] - Analysis of extracts of various cell lines, using PLCD4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PLCD4-Antibody-Western-Blot-NBP2-94312-img0002.jpg "Western Blot: PLCD4 Antibody [NBP2-94312] - Analysis of extracts of various cell lines, using PLCD4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")