| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98742-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99167-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90283-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99337-50ul | |||
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Immunocytochemistry analysis of U-937 cells labeling PI 3-Kinase p85 alpha with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32817_mouse-pi-3-kinase-p85-alpha-mab-a3-d0-r-29202410484840.jpg "Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Immunocytochemistry analysis of U-937 cells labeling PI 3-Kinase p85 alpha with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0-R) [NBP3-32817] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Mouse anti-PI 3-Kinase p85 alpha antibody (NBP3-32817) at 1/5,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: 293T cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: MCF7 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32817_mouse-pi-3-kinase-p85-alpha-mab-a3-d0-r-3920248485023.jpg)
![Western Blot: DGAT1 Antibody (JE34-88) [NBP3-32265] - Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32265) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32265_rabbit-dgat1-mab-je34-88-278202416331280.jpg "Western Blot: DGAT1 Antibody (JE34-88) [NBP3-32265] - Western blot analysis of DGAT1 on different lysates with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: MCF7 cell lysate (RIPA lysis) Lane 3: MCF7 cell lysate (hot lysis) Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32265) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DGAT1 Antibody (JE34-88) [NBP3-32265] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-DGAT1 antibody (NBP3-32265) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32265) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32265_rabbit-dgat1-mab-je34-88-29820241492691.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-98742] - Immunochemical staining of human GADD153/CHOP in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-Paraffin-NBP2-98742-img0002.jpg "Immunohistochemistry-Paraffin: GADD153/CHOP Antibody [NBP2-98742] - Immunochemical staining of human GADD153/CHOP in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-98742] - Immunofluorescence staining of GADD153/CHOP in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98742 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98742_rabbit-polyclonal-gadd153-chop-antibody-235202521525024.png)
![Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - WB Suggested Anti-GPX3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateGPX3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Western-Blot-NBP2-82233-img0002.jpg "Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - WB Suggested Anti-GPX3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateGPX3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells")
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 3/GPX3 Antibody [NBP2-82233] - Rabbit Anti-GPX3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue. Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells. Primary Antibody Concentration: 1:100. Other Working Concentrations: 1/600. Secondary Antibod](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-3-GPX3-Antibody-Immunohistochemistry-Paraffin-NBP2-82233-img0001.jpg)
![Immunohistochemistry-Paraffin: ACY3 Antibody [NBP2-99167] - Immunochemical staining of human ACY3 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ACY3-Antibody-Immunohistochemistry-Paraffin-NBP2-99167-img0002.jpg "Immunohistochemistry-Paraffin: ACY3 Antibody [NBP2-99167] - Immunochemical staining of human ACY3 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: ACY3 Antibody [NBP2-99167] - Immunofluorescence staining of ACY3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99167 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99167_rabbit-polyclonal-acy3-antibody-2352025213511.png)
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0002.jpg "Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).")
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Surface staining pattern of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0001.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (508) [NBP3-23485] - Immunochemical staining of human Lipocalin-2/NGAL in human lung with NBP3-23485 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23485_rabbit-lipocalin-2-ngal-mab-508-1362024149314.png "Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (508) [NBP3-23485] - Immunochemical staining of human Lipocalin-2/NGAL in human lung with NBP3-23485 at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (508) [NBP3-23485] - Immunochemical staining of human Lipocalin-2/NGAL in human ovarian cancer with NBP3-23485 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23485_rabbit-lipocalin-2-ngal-mab-508-12620241540462.png)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (508) [NBP3-23485] - Immunochemical staining of human Lipocalin-2/NGAL in human spleen with NBP3-23485 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23485_rabbit-lipocalin-2-ngal-mab-508-1262024134150.png)
![Western Blot: S100B Antibody (PD00-11) [NBP3-32924] - Western blot analysis of S100B on SK-MEL-28 cell lysates with Rabbit anti-S100B antibody (NBP3-32924) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32924) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32924_mouse-s100b-mab-pd00-11-288202412442930.jpg "Western Blot: S100B Antibody (PD00-11) [NBP3-32924] - Western blot analysis of S100B on SK-MEL-28 cell lysates with Rabbit anti-S100B antibody (NBP3-32924) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 1 minute; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32924) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: S100B Antibody (PD00-11) [NBP3-32924] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Mouse anti-S100B antibody (NBP3-32924) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32924) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32924_mouse-s100b-mab-pd00-11-298202414115432.jpg)
![Immunohistochemistry-Paraffin: SRD5A1 Antibody [NBP3-14482] - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/SRD5A1 Antibody-Immunohistochemistry-Paraffin-NBP3-14482-img0002.jpg "Immunohistochemistry-Paraffin: SRD5A1 Antibody [NBP3-14482] - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Simple Western: SRD5A1 Antibody [NBP3-14482] - SRD5A1 antibody (LS-A11212, 20 g/mL) yields a specific band on capillary Western analysis (Protein Simple, WES 12-230 kDa separation module) in 1 ng purified recombinant human SRD5A1 protein.](http://images.novusbio.com/fullsize/SRD5A1-Antibody-Simple-Western-NBP3-14482-img0001.jpg)
![Western Blot: FHIT Antibody (104) [NBP2-90283] - Anti-FHIT rabbit monoclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa.](http://images.novusbio.com/fullsize/FHIT-Antibody-104-Western-Blot-NBP2-90283-img0001.jpg "Western Blot: FHIT Antibody (104) [NBP2-90283] - Anti-FHIT rabbit monoclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa.")
![Immunocytochemistry/Immunofluorescence: FHIT Antibody (104) [NBP2-90283] - Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90283 antibody (1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-90283_rabbit-monoclonal-fhit-antibody-104-235202514371347.png)
![Immunohistochemistry-Paraffin: ABCD1 Antibody [NBP2-99337] - Immunochemical staining of human ABCD1 in human pancreas with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ABCD1-Antibody-Immunohistochemistry-Paraffin-NBP2-99337-img0002.jpg "Immunohistochemistry-Paraffin: ABCD1 Antibody [NBP2-99337] - Immunochemical staining of human ABCD1 in human pancreas with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/Immunofluorescence: ABCD1 Antibody [NBP2-99337] - Immunofluorescence staining of ABCD1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99337 antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99337_rabbit-polyclonal-abcd1-antibody-23520251220558.png)