| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00103-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
![Western Blot: CD133 Antibody (A8C3-R) [NBP3-32124] - Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (NBP3-32124) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32124) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-27820241631670.jpg)
![Immunohistochemistry: CD133 Antibody (A8C3-R) [NBP3-32124] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (NBP3-32124) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32124) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-298202414161867.jpg)
![Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-2882024142261.jpg "Western Blot: LAG-3 Antibody (PS01-33) [NBP3-32516] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/1,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 57/70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32516) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29820241416185.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PS01-33) [NBP3-32516] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG-3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32516) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32516_rabbit-lag-3-mab-ps01-33-29202410334064.jpg)
![Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-27820241529552.jpg "Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Immunocytochemistry analysis of Jurkat cells labeling TRAILR3/TNFRSF10C with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-2920241114817.jpg)
![Flow Cytometry: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Flow cytometric analysis of Jurkat cells labeling TRAILR3/TNFRSF10C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32257, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-29202416372111.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Immunohistochemistry-Paraffin: ZAG Antibody [NBP3-00103] - Immunochemical staining of human ZAG in human liver with NBP3-00103 at 1:2000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00103_rabbit-polyclonal-zag-antibody-235202511444457.png "Immunohistochemistry-Paraffin: ZAG Antibody [NBP3-00103] - Immunochemical staining of human ZAG in human liver with NBP3-00103 at 1:2000 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: ZAG Antibody [NBP3-00103] - NBP3-00103 at 1:500 dilution Lane A: COLO205 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:44 kDa](http://images.novusbio.com/fullsize/nbp3-00103_rabbit-polyclonal-zag-antibody-235202521451713.png)
![Immunohistochemistry-Paraffin: ZAG Antibody [NBP3-00103] - Immunochemical staining of human ZAG in human prostate with NBP3-00103 at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-00103_rabbit-polyclonal-zag-antibody-235202514492327.png)
![Western Blot: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32515) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-278202415212421.jpg "Western Blot: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 55/70 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32515) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32515) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-29820241435254.jpg)
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-44) [NBP3-32515] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32515) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32515_rabbit-lag-3-mab-psh0-44-2920241033400.jpg)
![Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS799] - Brain Hippocampus Neurons](http://images.novusbio.com/fullsize/Bradykinin-RB1-BDKRB1-Antibody-Immunohistochemistry-Paraffin-NLS799-img0005.jpg "Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS799] - Brain Hippocampus Neurons")
![Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS799] - Analysis of anti-BDKRB1 with human artery, smooth musche, atherosclerosis at dilution 5 ug/ml.](http://images.novusbio.com/fullsize/Bradykinin-RB1-BDKRB1-Antibody-Immunohistochemistry-Paraffin-NLS799-img0003.jpg)
![Immunohistochemistry-Paraffin: Bradykinin RB1/BDKRB1 Antibody [NLS799] - Analysis of anti-BDKRB1 antibody with human artery, smooth muscle, atherosclerosis at dilution 5 ug/ml.](http://images.novusbio.com/fullsize/Bradykinin-RB1-BDKRB1-Antibody-Immunohistochemistry-Paraffin-NLS799-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0003.jpg "Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.")
![Immunohistochemistry: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of human kidney](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-NBP2-31130-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0002.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0005.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the lymph node using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].")
![Immunohistochemistry-Paraffin: CD45 Antibody (E19-G) [NBP1-30145] - Expression in lymphoid cells of the palatine tonsil using formalin fixed, paraffin embedded human tissues. Staining with CD45 antibody (E19-G) [NBP1-30145].](http://images.novusbio.com/fullsize/CD45-Antibody-E19-G-Immunohistochemistry-Paraffin-NBP1-30145-img0003.jpg)
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202492029.jpg%3Fv=1666701381 "Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).")
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of HepG2 cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-10420249141939.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202491625.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024920223.jpg%3Fv=1666701381)
![ELISA: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024916215.jpg%3Fv=1666701381)
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)