| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04822-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03587-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05005-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97440-0.025mg | |||
![Western Blot: FCER1G Antibody [NBP3-04822] - Analysis of extracts of mouse spleen, using FCER1G antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FCER1G-Antibody-Western-Blot-NBP3-04822-img0001.jpg "Western Blot: FCER1G Antibody [NBP3-04822] - Analysis of extracts of mouse spleen, using FCER1G antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: MEKK3 Antibody (9N3Y9) [NBP3-16252] - Western blot analysis of extracts of various cell lines, using MEKK3 Rabbit mAb (NBP3-16252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MEKK3-Antibody-9N3Y9-Western-Blot-NBP3-16252-img0001.jpg "Western Blot: MEKK3 Antibody (9N3Y9) [NBP3-16252] - Western blot analysis of extracts of various cell lines, using MEKK3 Rabbit mAb (NBP3-16252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: CD36 Antibody (7P0S7) [NBP3-15616] - Western blot analysis of extracts of various cell lines, using CD36 antibody (NBP3-15616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/CD36-Antibody-7P0S7-Western-Blot-NBP3-15616-img0001.jpg "Western Blot: CD36 Antibody (7P0S7) [NBP3-15616] - Western blot analysis of extracts of various cell lines, using CD36 antibody (NBP3-15616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western Blot: IKK alpha Antibody (2M10E3) [NBP3-15862] - Western blot analysis of extracts of various cell lines, using IKK alpha antibody (NBP3-15862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-2M10E3-Western-Blot-NBP3-15862-img0001.jpg "Western Blot: IKK alpha Antibody (2M10E3) [NBP3-15862] - Western blot analysis of extracts of various cell lines, using IKK alpha antibody (NBP3-15862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: TLR5 Antibody (6P1Q10) [NBP3-16698] - Western blot analysis of extracts of various cell lines, using TLR5 Rabbit mAb (NBP3-16698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TLR5-Antibody-6P1Q10-Western-Blot-NBP3-16698-img0001.jpg "Western Blot: TLR5 Antibody (6P1Q10) [NBP3-16698] - Western blot analysis of extracts of various cell lines, using TLR5 Rabbit mAb (NBP3-16698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Complement C6 Antibody [NBP3-03587] - Analysis of extracts of various cell lines, using Complement C6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Complement-C6-Antibody-Western-Blot-NBP3-03587-img0001.jpg "Western Blot: Complement C6 Antibody [NBP3-03587] - Analysis of extracts of various cell lines, using Complement C6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: GBP1 Antibody [NBP3-05005] - Analysis of extracts of various cell lines, using GBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/GBP1-Antibody-Western-Blot-NBP3-05005-img0002.jpg "Western Blot: GBP1 Antibody [NBP3-05005] - Analysis of extracts of various cell lines, using GBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: ATF2 [p Thr52] Antibody [NBP2-29528] - Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Go6850. Image courtesy of Eric Lau and Ze'ev Ronai.](http://images.novusbio.com/fullsize/ATF2-[p-Thr52]-Antibody-Western-Blot-NBP2-29528-img0002.jpg "Western Blot: ATF2 [p Thr52] Antibody [NBP2-29528] - Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Go6850. Image courtesy of Eric Lau and Ze'ev Ronai.")
![Western Blot: Perforin Antibody (CE2.10) [NBP1-97440] - Detectioon of perforin in the mouse T cell clone B6.1. (lane 2). As control cell line HEK 293 was used (lane 1). The protein migrates around ~66kDa.Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10. antibody at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/Perforin-Antibody-CE2-10-Western-Blot-NBP1-97440-img0002.jpg "Western Blot: Perforin Antibody (CE2.10) [NBP1-97440] - Detectioon of perforin in the mouse T cell clone B6.1. (lane 2). As control cell line HEK 293 was used (lane 1). The protein migrates around ~66kDa.Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10. antibody at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.")
![Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33024_rabbit-tirap-mab-je46-35-288202412493424.jpg "Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: CD8 Antibody (LT8) [NBP3-09120] - Analysis (surface staining) of human peripheral blood cells with anti-human CD8 (LT8) purified / GAM-APC.](http://images.novusbio.com/fullsize/CD8-Antibody-LT8-Flow-Cytometry-NBP3-09120-img0001.jpg "Flow Cytometry: CD8 Antibody (LT8) [NBP3-09120] - Analysis (surface staining) of human peripheral blood cells with anti-human CD8 (LT8) purified / GAM-APC.")
![Western Blot: TNF-alpha Antibody (PS01-07) [NBP3-33032] - Western blot analysis of TNF-alpha on different lysates with Rabbit anti-TNF-alpha antibody (NBP3-33032) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100ng/mL LPS for 7 hours, add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26/50/17 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33032_rabbit-tnf-alpha-mab-ps01-07-288202412493475.jpg "Western Blot: TNF-alpha Antibody (PS01-07) [NBP3-33032] - Western blot analysis of TNF-alpha on different lysates with Rabbit anti-TNF-alpha antibody (NBP3-33032) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100ng/mL LPS for 7 hours, add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 26 kDa Observed band size: 26/50/17 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")