| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08732 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35109-1mg | |||
| Carrier-Free | NBP2-35109-100ug | |||
| Carrier-Free | NBP2-35109-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90452-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90272-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50521-0.025ml | |||
![Western Blot: iNOS Antibody (JE59-61) [NBP3-32472] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32472) at 1/1,000 dilution. Lane 1: RAW264.7 whole cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours whole cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32472_rabbit-inos-mab-je59-61-27820241631695.jpg "Western Blot: iNOS Antibody (JE59-61) [NBP3-32472] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32472) at 1/1,000 dilution. Lane 1: RAW264.7 whole cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours whole cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (4G7C3) [NBP3-16385] - Western blot analysis of extracts of various cell lines, using LYVE-1 Rabbit mAb (NBP3-16385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-4G7C3-Western-Blot-NBP3-16385-img0001.jpg "Western Blot: LYVE-1 Antibody (4G7C3) [NBP3-16385] - Western blot analysis of extracts of various cell lines, using LYVE-1 Rabbit mAb (NBP3-16385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![SDS-Page: Mouse IL-1 beta/IL-1F2 Protein [NBP2-35109]](http://images.novusbio.com/fullsize/Mouse-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35109-img0001.jpg "SDS-Page: Mouse IL-1 beta/IL-1F2 Protein [NBP2-35109]")
![Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-76806] - Staining of paraffin embedded Human peripheral blood leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Immunohistochemistry-Paraffin-NBP2-76806-img0001.jpg "Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-76806] - Staining of paraffin embedded Human peripheral blood leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunocytochemistry/Immunofluorescence: TREM2 Antibody (014) [NBP2-90452] - Immunofluorescence staining of TREM2 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90452 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/nbp2-90452_rabbit-monoclonal-trem2-antibody-014-23520251628190.png "Immunocytochemistry/Immunofluorescence: TREM2 Antibody (014) [NBP2-90452] - Immunofluorescence staining of TREM2 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90452 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.")
![Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-29202410334038.jpg "Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CD45 Antibody (PSH03-64) [NBP3-32448] - Flow cytometric analysis of human PBMC labeling human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32448, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-392024884642.jpg)
![Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Western-Blot-NBP2-90272-img0001.jpg "Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa")
![Immunoprecipitation: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Immunoprecipitation-NBP2-90272-img0002.jpg)
![Recombinant Human NLRP3/NALP3 Protein [H00114548-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00114548-Q01-1.jpg "Recombinant Human NLRP3/NALP3 Protein [H00114548-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Complement C3 Partial Recombinant Protein [H00000718-Q01]](http://images.novusbio.com/fullsize/Complement-C3-Partial-Recombinant-Protein-SDS-Page-H00000718-Q01-img0001.jpg "SDS-Page: Complement C3 Partial Recombinant Protein [H00000718-Q01]")
![Immunocytochemistry /Immunofluorescence: HLA G Antibody (mem-G/9) - BSA Free [NBP3-43587] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with NBP3-43587 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43587, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43587_rabbit-hla-g-mab-mem-g-9-azide-and-bsa-free-74202522113046.png "Immunocytochemistry /Immunofluorescence: HLA G Antibody (mem-G/9) - BSA Free [NBP3-43587] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with NBP3-43587 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43587, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.")