| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81084-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12062-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52641-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2701-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81083-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50162-0.025ml | |||
![Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-278202416285213.jpg "Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-2920241192322.jpg)
![Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-278202416331267.jpg "Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD3 Antibody (PD01-43) [NBP3-32140] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-29820241492648.jpg)
![Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-278202415295589.jpg "Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD79A Antibody (PDM0-23) [NBP3-32168] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD79A antibody (NBP3-32168) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32168) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-29820241435290.jpg)
![Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] - Immunocytochemical labeling of ATPase Na+/K+ beta 3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-ATPase Na+/K+ beta 3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23433_mouse-atpase-na-k-beta-3-mab-m016-19920231856962.jpg "Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] - Immunocytochemical labeling of ATPase Na+/K+ beta 3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-ATPase Na+/K+ beta 3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight(R) 594.")
![Western Blot: ATPase Na+/K+ beta 3 Antibody (M016) [NBP3-23433] - Western blot of NBP3-23433 immunoprecipitates (IP) and whole lysates. The NBP3-23433 antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-ATPase Na+/K+ beta 3 NBP3-23433 (lanes 1-7). The arrow designates native antibody, while the beta 3 subunit migrates around 40 kDa.](http://images.novusbio.com/fullsize/nbp3-23433_mouse-atpase-na-k-beta-3-mab-m016-19920231410112.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG-56-Chimeric-Flow-Cytometry-NBP2-81084-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG-56) - Chimeric [NBP2-81084] - Flow-cytometry using [NBP2-81084]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of DREG-56 (NBP2-81084, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)
![Western Blot: ATPase Na+/K+ beta 3 Antibody (M025) [NBP3-23434] - Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), and brain (lane 6) tissues. The blot was probed with anti-ATPase Na+/K+ beta 3 (NBP3-23434) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23434_mouse-atpase-na-k-beta-3-mab-m025-19920231410140.jpg "Western Blot: ATPase Na+/K+ beta 3 Antibody (M025) [NBP3-23434] - Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), and brain (lane 6) tissues. The blot was probed with anti-ATPase Na+/K+ beta 3 (NBP3-23434) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody (M025) [NBP3-23434] - Immunocytochemical labeling of ATPase Na+/K+ beta 3 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal anti-ATPase Na+/K+ beta 3 (clone M025). The antibody was detected using goat anti-mouse Ig DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23434_mouse-atpase-na-k-beta-3-mab-m025-19920231252362.jpg)
![Immunocytochemistry/Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric [NBP3-12062] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of TL2.1 (NBP3-12062) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12062, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12062-img0001.jpg "Immunocytochemistry/Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric [NBP3-12062] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of TL2.1 (NBP3-12062) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12062, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-MEL-14-Flow-Cytometry-NBP2-81083-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) - Chimeric - Azide and BSA Free [NBP2-81083] - Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or NBP2-81083 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp2-81083_rabbit-monoclonal-l-selectin-cd62l-antibody-mel-14-74202522141216.png)