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![Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.](http://images.novusbio.com/fullsize/IL-21-Antibody-MT216G-21-3m-Neutralization-NBP3-18290-img0001.jpg "Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.")
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Validation in Human HL-60 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: IL-21 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740pep_il-21-antibody-blocking-peptide-2711202419251583.jpg "Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Validation in Human HL-60 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: IL-21 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.")
![SDS-Page: Recombinant Human BOB1 Protein [NBP1-72368] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-BOB1-Protein-SDS-Page-NBP1-72368-img0001.jpg "SDS-Page: Recombinant Human BOB1 Protein [NBP1-72368] - 15% SDS-PAGE (3ug)")
![SDS-Page: Recombinant Human BCMA/TNFRSF17 Protein [NBP2-52270]](http://images.novusbio.com/fullsize/Recombinant-Human-BCMA-TNFRSF17-Protein-SDS-Page-NBP2-52270-img0002.jpg "SDS-Page: Recombinant Human BCMA/TNFRSF17 Protein [NBP2-52270]")