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![Western Blot: RAG2 Antibody [NBP3-38173] - Western blot analysis of various lysates using [KO Validated] RAG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38173_rabbit-rag2-pab-101220241243961.jpg "Western Blot: RAG2 Antibody [NBP3-38173] - Western blot analysis of various lysates using [KO Validated] RAG2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: RAG2 Antibody [NBP3-38173] - Western Blot analysis of lysates from wild type (WT) and RAG2 knockout (KO) 293T cells, using [KO Validated] RAG2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38173_rabbit-rag2-pab-3112025173199.jpg)
![Immunohistochemistry: CD20 Antibody (PD00-02) [NBP3-32131] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD20 antibody (NBP3-32131) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32131) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32131_rabbit-cd20-mab-pd00-02-29820241435213.jpg "Immunohistochemistry: CD20 Antibody (PD00-02) [NBP3-32131] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD20 antibody (NBP3-32131) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32131) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: IL-13R alpha 1 Antibody [NBP2-16943] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL-13R alpha 1 antibody [N1C1] (NBP2-16943) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16943_rabbit-polyclonal-il-13r-alpha-1-antibody-910202420569.jpg "Western Blot: IL-13R alpha 1 Antibody [NBP2-16943] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL-13R alpha 1 antibody [N1C1] (NBP2-16943) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![IL13 Receptor alpha 1 antibody [N1C1] detects IL13 Receptor alpha 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.IL13 Receptor alpha 1 stained by IL13 Receptor alpha 1 antibody [N1C1] (NBP2-16943) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16943_rabbit-polyclonal-il-13r-alpha-1-antibody-immunohistochemistry-paraffin-261120251848482.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (C5/473) [NBP2-32940] - Formalin-fixed, paraffin-embedded human tonsil stained with CD5 monoclonal antibody (C5/473).](http://images.novusbio.com/fullsize/CD5-Antibody-C5-473-Immunohistochemistry-Paraffin-NBP2-32940-img0001.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (C5/473) [NBP2-32940] - Formalin-fixed, paraffin-embedded human tonsil stained with CD5 monoclonal antibody (C5/473).")
![Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14904] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [N2C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-Western-Blot-NBP2-14904-img0003.jpg "Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14904] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [N2C3] diluted at 1:500.")
![Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14904] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-Western-Blot-NBP2-14904-img0002.jpg)
![Immunohistochemistry-Paraffin: CD72 Antibody (C7) [NBP3-41620] - DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 20ug/ml NBP3-41620; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41620_mouse-cd72-mab-c7-612202421304365.jpg "Immunohistochemistry-Paraffin: CD72 Antibody (C7) [NBP3-41620] - DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 20ug/ml NBP3-41620; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: RAG2 Antibody [H00005897-D01P] - Analysis of RAG2 expression in rat liver.](http://images.novusbio.com/fullsize/RAG2-Antibody-Western-Blot-H00005897-D01P-img0002.jpg "Western Blot: RAG2 Antibody [H00005897-D01P] - Analysis of RAG2 expression in rat liver.")
![Western Blot: RAG2 Antibody [H00005897-D01P] - Analysis of RAG2 expression in transfected 293T cell line by RAG2 polyclonal antibody.Lane 1: RAG2 transfected lysate(59.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RAG2-Antibody-Western-Blot-H00005897-D01P-img0003.jpg)
![Western Blot: RAG2 Antibody (2G8) [H00005897-M06] - Analysis of RAG2 expression in rat liver.](http://images.novusbio.com/fullsize/RAG2-Antibody-2G8-Western-Blot-H00005897-M06-img0002.jpg "Western Blot: RAG2 Antibody (2G8) [H00005897-M06] - Analysis of RAG2 expression in rat liver.")
![ELISA: RAG2 Antibody (2G8) [H00005897-M06] - Detection limit for recombinant GST tagged RAG2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAG2-Antibody-2G8-ELISA-H00005897-M06-img0001.jpg)
