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![Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33024_rabbit-tirap-mab-je46-35-288202412493424.jpg "Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Viperin Antibody [NBP2-89062] - Detection of mouse Viperin in L929 cells by Western Blot using PAb to Viperin (AT131) Method: Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3ug/ml (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the PAb to Viperin (AT131) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to rabbit IgG and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/Viperin-Antibody-Western-Blot-NBP2-89062-img0001.jpg "Western Blot: Viperin Antibody [NBP2-89062] - Detection of mouse Viperin in L929 cells by Western Blot using PAb to Viperin (AT131) Method: Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3ug/ml (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the PAb to Viperin (AT131) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to rabbit IgG and a chemiluminescence detection system.")
![Western Blot: DcR3 Antibody [NBP2-16112] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DcR3-Antibody-Western-Blot-NBP2-16112-img0001.jpg "Western Blot: DcR3 Antibody [NBP2-16112] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: TLR5 Antibody (C8) [NBP3-41500] - DAB staining on IHC-P; Sample: Rat Lymph node Tissue; Primary Ab: 30ug/ml NBP3-41500; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41500_mouse-tlr5-mab-c8-612202421331612.jpg "Immunohistochemistry-Paraffin: TLR5 Antibody (C8) [NBP3-41500] - DAB staining on IHC-P; Sample: Rat Lymph node Tissue; Primary Ab: 30ug/ml NBP3-41500; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: TLR1 Antibody [NBP3-12901] - Jurkat whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR1 antibody (NBP3-12901) diluted at 1:1000.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NBP3-12901-img0001.jpg "Western Blot: TLR1 Antibody [NBP3-12901] - Jurkat whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TLR1 antibody (NBP3-12901) diluted at 1:1000.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-10995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10995-img0001.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-10995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBL1-13949] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13949-img0002.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBL1-13949] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11265] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11265-img0001.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11265] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: TLR4 Overexpression Lysate [NBP2-05647] - (Adult Normal) [NBP2-05647] Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate). Right lane: Over-expression Lysate for TLR4.](http://images.novusbio.com/fullsize/TLR4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05647-img0001.jpg "Western Blot: TLR4 Overexpression Lysate [NBP2-05647] - (Adult Normal) [NBP2-05647] Left lane: Empty vector transfected control cell lysate (HEK293 cell lysate). Right lane: Over-expression Lysate for TLR4.")