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![Western Blot: p53 [p Ser9] Antibody (10U5R6) [NBP3-16909] - Western blot analysis of extracts of NIH/3T3 cells, using p53 Rabbit mAb (NBP3-16909) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/p53-p-Ser9-Antibody-10U5R6-Western-Blot-NBP3-16909-img0001.jpg "Western Blot: p53 [p Ser9] Antibody (10U5R6) [NBP3-16909] - Western blot analysis of extracts of NIH/3T3 cells, using p53 Rabbit mAb (NBP3-16909) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Western Blot: APOJ / Clusterin Overexpression Lysate (Adult Normal) [NBP2-04577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for APOJ / Clusterin.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04577-img0001.jpg "Western Blot: APOJ / Clusterin Overexpression Lysate (Adult Normal) [NBP2-04577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for APOJ / Clusterin.")
![Western Blot: S100B Overexpression Lysate (Adult Normal) [NBL1-15661] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for S100B.](http://images.novusbio.com/fullsize/S100B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15661-img0002.jpg "Western Blot: S100B Overexpression Lysate (Adult Normal) [NBL1-15661] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for S100B.")
![Western Blot: AKT1 Overexpression Lysate (Adult Normal) [NBP2-04280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT1.](http://images.novusbio.com/fullsize/AKT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04280-img0001.jpg "Western Blot: AKT1 Overexpression Lysate (Adult Normal) [NBP2-04280] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKT1.")
![Western Blot: p53 Overexpression Lysate (Adult Normal) [NBP2-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53.](http://images.novusbio.com/fullsize/p53-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11392-img0001.jpg "Western Blot: p53 Overexpression Lysate (Adult Normal) [NBP2-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53.")
![Western Blot: GSK-3 alpha Antibody (6G12C2) [NB110-87049] - Analysis using GSK3 alpha mouse mAb against truncated GSK3 alpha recombinant protein (1)and Hela cell lysate (2).](http://images.novusbio.com/fullsize/GSK-3-alpha-Antibody-6G12C2-Western-Blot-NB110-87049-img0003.jpg "Western Blot: GSK-3 alpha Antibody (6G12C2) [NB110-87049] - Analysis using GSK3 alpha mouse mAb against truncated GSK3 alpha recombinant protein (1)and Hela cell lysate (2).")
![Western Blot: HSP70/HSPA1A [p Ser153] Antibody [NBP2-60773] - Western blot of T47D cell lysate treated with EGF (1nM) for 60minutes showing specific labeling of the ~70 kDa Hsp70 in thefirst lane (-). Phosphospecificity is shown in the second lane(+) where immunolabeling is eliminated or significantlydecreased with lambda phosphatase (Lambda-Ptase, 1200 units for30 minutes).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[p-Ser153]-Antibody-Western-Blot-NBP2-60773-img0001.jpg "Western Blot: HSP70/HSPA1A [p Ser153] Antibody [NBP2-60773] - Western blot of T47D cell lysate treated with EGF (1nM) for 60minutes showing specific labeling of the ~70 kDa Hsp70 in thefirst lane (-). Phosphospecificity is shown in the second lane(+) where immunolabeling is eliminated or significantlydecreased with lambda phosphatase (Lambda-Ptase, 1200 units for30 minutes).")
![Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779] - Western blot analysis of TOR/mTOR on different lysates with Rabbit anti-TOR/mTOR antibody (NBP3-32779) at 1/5,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32779) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32779_rabbit-tor-mtor-p-ser2481-mab-je59-62-288202412461671.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779] - Western blot analysis of TOR/mTOR on different lysates with Rabbit anti-TOR/mTOR antibody (NBP3-32779) at 1/5,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32779) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: TRIB3 Antibody [NBP3-14503] - Immunohistochemistry of formalin-fixed, paraffin-embedded human breast after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TRIB3 Antibody-Immunohistochemistry-Paraffin-NBP3-14503-img0001.jpg "Immunohistochemistry-Paraffin: TRIB3 Antibody [NBP3-14503] - Immunohistochemistry of formalin-fixed, paraffin-embedded human breast after heat-induced antigen retrieval.")
![Western Blot: Nox4 Antibody [NBP2-99109] - Anti-Nox4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa](http://images.novusbio.com/fullsize/Nox4-Antibody-Western-Blot-NBP2-99109-img0001.jpg "Western Blot: Nox4 Antibody [NBP2-99109] - Anti-Nox4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NBP2-97994] - Immunofluorescence staining of GSK-3 alpha in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97994 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97994_rabbit-polyclonal-gsk-3-alpha-antibody-235202511444439.png "Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NBP2-97994] - Immunofluorescence staining of GSK-3 alpha in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97994 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")