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![Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/RPTOR-Antibody-1B10-Immunohistochemistry-NBP3-15202-img0001.jpg "Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/RPTOR-Antibody-1B10-Immunohistochemistry-NBP3-15202-img0002.jpg)
![Immunoprecipitation: TSC2 Antibody [NB200-200] - Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody NB200-200 used for IP at 3 ug per reaction. TSC2 was also immunoprecipitated by another rabbit anti-TSC2 antibody. For blotting immunoprecipitated TSC2, NB200-200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TSC2-Antibody-Immunoprecipitation-NB200-200-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: TSC2 Antibody [NB200-200] - Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody NB200-200 used for IP at 3 ug per reaction. TSC2 was also immunoprecipitated by another rabbit anti-TSC2 antibody. For blotting immunoprecipitated TSC2, NB200-200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")