| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-130SS | |||
| Azide and BSA Free | NBP2-80761 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69088SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-49470AFP488 | |||
| Alexa Fluor Plus 594 | NBP3-49470AFP594 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08388 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24095 | |||
![Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-H1alpha-67-7-Western-Blot-NB100-130-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.")
![Immunohistochemistry-Paraffin: MBP Antibody (HL1033) [NBP3-13687] - MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/MBP-Antibody-HL1033-Immunohistochemistry-Paraffin-NBP3-13687-img0002.jpg "Immunohistochemistry-Paraffin: MBP Antibody (HL1033) [NBP3-13687] - MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![MBP antibody [HL1033] detects MBP protein at cell membrane by immunohistochemical analysis.</br>Sample: Frozen-sectioned mouse cerebellum.</br>Green: MBP stained by MBP antibody [HL1033] (NBP3-13687) diluted at 1:100.</br>Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-910202420122087.jpg)
![Myelin basic protein antibody [HL1033] detects Myelin basic protein protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded rat hippocampus.</br>Myelin basic protein stained by Myelin basic protein antibody [HL1033] (NBP3-13687) diluted at 1:200.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-immunohistochemistry-paraffin-261120251834262.jpg)
![Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic protein antibody [HL1033] (NBP3-13687) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-western-blot-261120251829269.jpg)
![Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Myelin basic Protein antibody [HL1033] (NBP3-13687) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13687_rabbit-mbp-mab-hl1033-western-blot-261120251831289.jpg)
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![ELISA: Rat MBP ELISA Kit (Colorimetric) [NBP2-81167] - Samples were spiked with high concentrations of Rat MBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81167_rat-mbp-elisa-kit-colorimetric-13220241612131.jpg "ELISA: Rat MBP ELISA Kit (Colorimetric) [NBP2-81167] - Samples were spiked with high concentrations of Rat MBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat MBP ELISA Kit (Colorimetric) [NBP2-81167] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81167_rat-mbp-elisa-kit-colorimetric-432024955057.jpg)
![Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Dimethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69089-img0002.jpg "Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.")
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate<br/> Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate<br/> Lane 3: HepG2 cell lysate<br/> Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 93 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 1minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate<br/> Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate<br/> Lane 3: HepG2 cell lysate<br/> Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 93 kDa<br/> Observed band size: 120 kDa<br/><br/> Exposure time: 1minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-07276] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).](http://images.novusbio.com/fullsize/MBP-Antibody-MBP-4277R-Immunohistochemistry-Paraffin-NBP3-07276-img0003.jpg "Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-07276] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).")
![Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-07276] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).](http://images.novusbio.com/fullsize/MBP-Antibody-MBP-4277R-Immunohistochemistry-Paraffin-NBP3-07276-img0002.jpg)
![Protein Array: MBP Antibody (MBP/4277R) [NBP3-07276] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).](http://images.novusbio.com/fullsize/MBP-Antibody-MBP-4277R-Protein-Array-NBP3-07276-img0001.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (rMBP/9301) [NBP3-23738] - Formalin-fixed, paraffin-embedded human brain stained with MBP Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23738_mouse-mbp-mab-rmbp-9301-2412025872327.jpg "Immunohistochemistry-Paraffin: MBP Antibody (rMBP/9301) [NBP3-23738] - Formalin-fixed, paraffin-embedded human brain stained with MBP Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.")
