| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69088SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08388 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92950-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24095 | |||
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Dimethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69089-img0002.jpg "Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.")
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-07276] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).](http://images.novusbio.com/fullsize/MBP-Antibody-MBP-4277R-Immunohistochemistry-Paraffin-NBP3-07276-img0003.jpg "Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-07276] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).")
![Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-07276] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).](http://images.novusbio.com/fullsize/MBP-Antibody-MBP-4277R-Immunohistochemistry-Paraffin-NBP3-07276-img0002.jpg)
![Protein Array: MBP Antibody (MBP/4277R) [NBP3-07276] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).](http://images.novusbio.com/fullsize/MBP-Antibody-MBP-4277R-Protein-Array-NBP3-07276-img0001.jpg)
![Western Blot: EEF2K Antibody [NBP3-23302] - Western blot image of EEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of EEF2K from HeLa cell lysate using anti-EEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-EEF2K (C-terminus) (lanes 1-5) or with anti-EEF2K (N-terminal) antibody (lanes 6 & 7).](http://images.novusbio.com/fullsize/nbp3-23302_rabbit-eef2k-pab-19920231856952.jpg "Western Blot: EEF2K Antibody [NBP3-23302] - Western blot image of EEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of EEF2K from HeLa cell lysate using anti-EEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-EEF2K (C-terminus) (lanes 1-5) or with anti-EEF2K (N-terminal) antibody (lanes 6 & 7).")
![Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP3-23302] - Immunocytochemical labeling of EEF2K in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rabbit polyclonal anti-EEF2K (NBP3-23302). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23302_rabbit-eef2k-pab-199202318202919.jpg)
![Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts of various cell lines, using EEF2K at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-NBP2-92950-img0003.jpg "Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts of various cell lines, using EEF2K at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.")
![Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP2-92950] - Analysis of A-549 cells using EEF2K . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92950-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/EEF2K-Antibody-Knockout-Validated-NBP2-92950-img0004.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody (rMBP/9301) [NBP3-23738] - Formalin-fixed, paraffin-embedded human brain stained with MBP Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23738_mouse-mbp-mab-rmbp-9301-2412025872327.jpg "Immunohistochemistry-Paraffin: MBP Antibody (rMBP/9301) [NBP3-23738] - Formalin-fixed, paraffin-embedded human brain stained with MBP Antibody (rMBP/9301). Inset: PBS instead of primary antibody; secondary only negative control.")
