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![Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Western blot shows lysates of HepG2 human liver carcinoma parental cell line and GDF-15 knockout (KO) HepG2 cell line. PVDF membrane was probed with 1:250 of Rat Anti-Human GDF-15 Polyclonal Antibody (Catalog # NBP2-44214) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog #HAF006). Specific band was detected for GDF-15 at approximately 33 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/GDF-15-Antibody-6D12-H10-E4-Knockdown-Validated-NBP2-44214-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Western blot shows lysates of HepG2 human liver carcinoma parental cell line and GDF-15 knockout (KO) HepG2 cell line. PVDF membrane was probed with 1:250 of Rat Anti-Human GDF-15 Polyclonal Antibody (Catalog # NBP2-44214) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog #HAF006). Specific band was detected for GDF-15 at approximately 33 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Analysis of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained. Lane 2: MBP-NAG-1 fusion protein 1. Lane 3: MBP-NAG-1 fusion protein 2. Lane 4: MBP. Load 40ng. Primary Antibody: Rat anti-Nag 1 (C terminal specific). Secondary Antibody: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed.](http://images.novusbio.com/fullsize/GDF-15-Antibody-6D12-H10-E4-Western-Blot-NBP2-44214-img0001.jpg)
