| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB120-5888 | |||
| Biotin | NB120-5886 | |||
| PE | NB120-5889 | |||
| Rhodamine | NB120-5885 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NB120-5885 | |||
| Pre-adsorbed | NB120-5886 | |||
| Pre-adsorbed | NB120-5889 | |||
| Pre-adsorbed | NB120-5888 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72607 | |||
| Biotin | NBP1-73402 | |||
| FITC | NBP1-73209 | |||
| Rhodamine | NBP1-73208 | |||
| Texas Red | NBP1-73403 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-97178 | |||
| Biotin | NBP1-96952 | |||
| FITC | NBP1-96860 | |||
| HRP | NBP1-96818 | |||
| Rhodamine | NBP1-96861 | |||
| Texas Red | NBP1-96953 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP1-96818 | |||
| Azide and BSA Free | NBP1-97178 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-97093 | |||
| Biotin | NBP1-96907 | |||
| FITC | NBP1-96917 | |||
| Rhodamine | NBP1-96919 | |||
| Texas Red | NBP1-96905 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-96919 | |||
| Azide and BSA Free | NBP1-97093 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96900 | |||
| HRP | NBP1-96883 | |||
| Rhodamine | NBP1-96913 | |||
| Texas Red | NBP1-96899 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP3-11660 | |||
| DyLight 680 | NBP3-11664 | |||
| DyLight 800 | NBP3-11663 | |||
| FITC | NBP3-11662 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| PerCP/Cy5.5 | NBP1-42836 | |||
![Western Blot: Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-5882] - Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Mouse IgG F(ab')2. Other band(s): None. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Unconjugated-Pre-adsorbed-Western-Blot-NB120-5882-img0001.jpg "Western Blot: Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-5882] - Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Mouse IgG F(ab')2. Other band(s): None. Image from the PE version of this antibody.")
![ELISA: Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-72785] - ELISA Results of Rabbit anti-Human IgG F(ab')2 Secondary antibody (Pre-adsorbed) (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab')2 Mx Ms. Each well was coated in duplicate with 1.0 ug of Human IgG F(ab')2 [Red Line], Human IgG [Green Line], and Mouse IgG [Blue Line]. The working dilution of Human IgG F(ab')2 MX is 1:56300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG-F-ab-2-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72785-img0001.jpg "ELISA: Rabbit anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-72785] - ELISA Results of Rabbit anti-Human IgG F(ab')2 Secondary antibody (Pre-adsorbed) (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab')2 Mx Ms. Each well was coated in duplicate with 1.0 ug of Human IgG F(ab')2 [Red Line], Human IgG [Green Line], and Mouse IgG [Blue Line]. The working dilution of Human IgG F(ab')2 MX is 1:56300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit HRP conjugated, and TMB substrate.")
![Dot Blot: Goat anti-Bovine IgG F(ab')2 Secondary Antibody [NBP1-72717] - Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Bovine-IgG-F-ab-2-Secondary-Antibody-Pre-absorbed-Dot-Blot-NBP1-72717-img0001.jpg "Dot Blot: Goat anti-Bovine IgG F(ab')2 Secondary Antibody [NBP1-72717] - Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Blocked with blocking buffer for 60 min at RT. Image from the FITC version of this antibody.")
![Western Blot: Chicken IgG Isotype Control [NBP1-97037] - SDS-Page: Chicken IgG Isotype Control [Agarose Immobilized] [NBP1-97189] - Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Image using the Agarose form of this antibody.](http://images.novusbio.com/fullsize/Chicken-IgG-Isotype-Control-Western-Blot-NBP1-97037-img0001.jpg "Western Blot: Chicken IgG Isotype Control [NBP1-97037] - SDS-Page: Chicken IgG Isotype Control [Agarose Immobilized] [NBP1-97189] - Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Image using the Agarose form of this antibody.")
![SDS-Page: Bovine IgG Isotype Control [NBP1-97052] - SDS PAGE Results of Bovine IgG isotype control. Lane 1: Bovine IgG Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Non-Bovine IgG Reduced. 4-20% Gel, Coomassie Stained.](http://images.novusbio.com/fullsize/Bovine-IgG-Isotype-Control-SDS-Page-NBP1-97052-img0001.jpg "SDS-Page: Bovine IgG Isotype Control [NBP1-97052] - SDS PAGE Results of Bovine IgG isotype control. Lane 1: Bovine IgG Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Non-Bovine IgG Reduced. 4-20% Gel, Coomassie Stained.")
![SDS-Page: Equine IgG Isotype Control [NBP1-97045] - SDS PAGE of Horse IgG Whole Molecule. Lane 1: Non-Reduced Horse IgG Whole Molecule. Lane 2: 5uL Opal Prestained Marker. Lane 3: Reduced Horse IgG Whole Molecule. Load: 1ug per lane.](http://images.novusbio.com/fullsize/Equine IgG Isotype Control-SDS-Page-NBP1-97045-img0002.jpg "SDS-Page: Equine IgG Isotype Control [NBP1-97045] - SDS PAGE of Horse IgG Whole Molecule. Lane 1: Non-Reduced Horse IgG Whole Molecule. Lane 2: 5uL Opal Prestained Marker. Lane 3: Reduced Horse IgG Whole Molecule. Load: 1ug per lane.")
![Western Blot: Protein A Antibody [NBP2-53424] - Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: approx. 50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.](http://images.novusbio.com/fullsize/Protein-A-Antibody-Western-Blot-NBP2-53424-img0001.jpg "Western Blot: Protein A Antibody [NBP2-53424] - Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: approx. 50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.")
![Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of A. 30 ug 293T whole cell lysate/eztract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-247-Western-Blot-NBP2-43726-img0002.jpg "Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of A. 30 ug 293T whole cell lysate/eztract B. 30 ug whole cell lysate/extract of S-tagged protein expressing 293T cells 12 % SDS-PAGE S Tag antibody dilution: 1:5000.")
![Western Blot: S-Tag Epitope Tag Antibody (247) [NBP2-43726] - Analysis of 400 ug cell lysate/extract of S-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of S-fused protein by 2 ug of S tag antibody 12% SDS-PAGE The immunoprecipitated S-fused protein was detected by S tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-247-Western-Blot-NBP2-43726-img0001.jpg)
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0002.jpg "Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.")
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] detects only native antibody and not denatured heavy and light chains.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0001.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833] - Analysis of recombinant protein containing the epitope tag sequence DYKDDDDK using DYKDDDDK Epitope Tag Antibody [NBP2-37833].](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NBP2-37833-img0002.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833] - Analysis of recombinant protein containing the epitope tag sequence DYKDDDDK using DYKDDDDK Epitope Tag Antibody [NBP2-37833].")