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![Western Blot: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Lane 1: HeLa cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH3T3 cell lysates, Lane 4: Raw 264.7 cell lysates, Lane 5: LNCaP cell lysates](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-9E9-Western-Blot-NBP2-59431-img0001.jpg "Western Blot: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Lane 1: HeLa cell lysates, Lane 2: HepG2 cell lysates, Lane 3: NIH3T3 cell lysates, Lane 4: Raw 264.7 cell lysates, Lane 5: LNCaP cell lysates")
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (9E9) [NBP2-59431] - Analysis of PDIA3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDIA3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-9E9-Immunocytochemistry-Immunofluorescence-NBP2-59431-img0002.jpg)
![SDS-Page: Recombinant Human HSP90 beta Protein [NBP3-18320] - SDS-PAGE of 90kDa HSP90 beta protein (NBP3-18320). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HSP90 beta protein (NBP3-18320).](http://images.novusbio.com/fullsize/Recombinant Human HSP90 beta Protein-SDS-Page-NBP3-18320-img0001.jpg "SDS-Page: Recombinant Human HSP90 beta Protein [NBP3-18320] - SDS-PAGE of 90kDa HSP90 beta protein (NBP3-18320). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HSP90 beta protein (NBP3-18320).")
![Immunohistochemistry;ERp57/PDIA3 Antibody[NBP3-29115]Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 used at a dilution of 1:5,000 . Detection: DAB. Counterstain:IHC Hematoxylin .](http://images.novusbio.com/fullsize/nbp3-29116_rabbit-erp57-pdia3-pab-782024936128.jpg "Immunohistochemistry;ERp57/PDIA3 Antibody[NBP3-29115]Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 used at a dilution of 1:5,000 . Detection: DAB. Counterstain:IHC Hematoxylin .")
![Immunohistochemistry;ERp57/PDIA3 Antibody[NBP3-29141]Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 used at a dilution of 1:5,000 . Detection: DAB. Counterstain: IHC Hematoxylin .](http://images.novusbio.com/fullsize/nbp3-29142_rabbit-erp57-pdia3-pab-7820241039266.jpg "Immunohistochemistry;ERp57/PDIA3 Antibody[NBP3-29141]Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 used at a dilution of 1:5,000 . Detection: DAB. Counterstain: IHC Hematoxylin .")
![Western Blot: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394] - Western blot analysis of extracts of various cell lines, using ERp57/PDIA3 Rabbit mAb (NBP3-16394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-9S9X4-Western-Blot-NBP3-16394-img0002.jpg "Western Blot: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394] - Western blot analysis of extracts of various cell lines, using ERp57/PDIA3 Rabbit mAb (NBP3-16394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394] - Immunohistochemistry of paraffin-embedded human placenta using ERp57/PDIA3 Rabbit mAb (NBP3-16394) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-9S9X4-Immunohistochemistry-Paraffin-NBP3-16394-img0001.jpg)
![Immunohistochemistry: HSP90 beta Antibody (7F4) [NBP3-26215] - Image of HSP90 beta Antibody (7F4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.](http://images.novusbio.com/fullsize/nbp3-26215_rabbit-hsp90-beta-mab-7f4-262202414471543.jpg "Immunohistochemistry: HSP90 beta Antibody (7F4) [NBP3-26215] - Image of HSP90 beta Antibody (7F4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7F4) [NBP3-26215] - Image of HSP90 beta Antibody (7F4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.74% DAB.](http://images.novusbio.com/fullsize/nbp3-26215_rabbit-hsp90-beta-mab-7f4-26220241422529.jpg)
![Flow Cytometry: HSP90 beta Antibody (7F4) [NBP3-26215] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7F4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26215_rabbit-hsp90-beta-mab-7f4-282202414183699.jpg)