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![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCAK antibody [N1N3] (NBP2-17054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17054_rabbit-polyclonal-kif2c-antibody-western-blot-261120251974515.jpg "Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCAK antibody [N1N3] (NBP2-17054) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![MCAK antibody [N1N3] detects MCAK protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MCAK protein stained by MCAK antibody [N1N3] (NBP2-17054) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-17054_rabbit-polyclonal-kif2c-antibody-immunocytochemistry-immunofluorescence-261120251839615.jpg)
![Western Blot: MANF Antibody [NBP1-32037] - ARMET Antibody [NBP1-32037] - Sample(30 ug of whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/ARMET-Antibody-Western-Blot-NBP1-32037-img0003.jpg "Western Blot: MANF Antibody [NBP1-32037] - ARMET Antibody [NBP1-32037] - Sample(30 ug of whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1500.")
![Immunocytochemistry/Immunofluorescence: ARMET Antibody [NBP1-32037] - Analysis of methanol-fixed HepG2, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32037-img0002.jpg)
![Western Blot: FANCE Antibody [NBP2-16428] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FANCE-Antibody-Western-Blot-NBP2-16428-img0001.jpg "Western Blot: FANCE Antibody [NBP2-16428] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FANCE Antibody [NBP2-16428] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/FANCE-Antibody-Immunohistochemistry-Paraffin-NBP2-16428-img0002.jpg)
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Detection of Human and Mouse BLM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-1590 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-1590-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Detection of Human and Mouse BLM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-1590 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Whole cell lysate from mouse NIH3T3 cells. BLM was also immunoprecipitated using rabbit anti-BLM antibodies NB100-1588 and NB100-1589.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-1590-img0001.jpg)
![Western Blot: CPEB1 Antibody [NBP2-15981] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CPEB1-Antibody-Western-Blot-NBP2-15981-img0001.jpg "Western Blot: CPEB1 Antibody [NBP2-15981] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CPEB1 Antibody [NBP2-15981] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/CPEB1-Antibody-Immunohistochemistry-Paraffin-NBP2-15981-img0002.jpg)
![Western Blot: CPEB1 Antibody [NBP2-15982] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/CPEB1-Antibody-Western-Blot-NBP2-15982-img0001.jpg "Western Blot: CPEB1 Antibody [NBP2-15982] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: CPEB1 Antibody [NBP2-15982] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CPEB1-Antibody-Immunohistochemistry-Paraffin-NBP2-15982-img0002.jpg)
![Western Blot: NEI3 Antibody [NBP3-42057] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-42057_rabbit-nei3-pab-612202421304364.jpg "Western Blot: NEI3 Antibody [NBP3-42057] - Sample: Recombinant protein.")
![Immunohistochemistry-Paraffin: NEI3 Antibody [NBP3-42057] - DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 20ug/ml NBP3-42057; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42057_rabbit-nei3-pab-61220242133166.jpg)
![Western Blot: CPEB1 Antibody [NBP2-15980] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CPEB1-Antibody-Western-Blot-NBP2-15980-img0001.jpg "Western Blot: CPEB1 Antibody [NBP2-15980] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.")
![CPEB1 antibody [C3], C-term detects CPEB1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CPEB1 stained by CPEB1 antibody [C3], C-term (NBP2-15980) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-15980_rabbit-polyclonal-cpeb1-antibody-immunocytochemistry-immunofluorescence-2611202518384021.jpg)
![Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0058.jpg "Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MCM2 Antibody [NB100-244] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-244-img0057.jpg)
![Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB600-487-img0007.jpg "Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NB600-487] - Nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-487-img0005.jpg)
![Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000](http://images.novusbio.com/fullsize/USP7-Antibody-6512-Western-Blot-NBP2-43619-img0001.jpg "Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000")
![USP7 antibody [GT6512] detects USP7 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP7 protein stained by USP7 antibody [GT6512] (NBP2-43619) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43619_mouse-monoclonal-usp7-antibody-6512-immunocytochemistry-immunofluorescence-2611202518342614.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody [6196] (NBP2-43556) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43556_mouse-monoclonal-dna-polymerase-delta-catalytic-subunit-antibody-6196-western-blot-261120251841134.jpg "Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA polymerase delta antibody [6196] (NBP2-43556) diluted at 1:1000.")
![DNA polymerase delta antibody [6196] detects DNA polymerase delta protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.DNA polymerase delta stained by DNA polymerase delta antibody [6196] (NBP2-43556) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43556_mouse-monoclonal-dna-polymerase-delta-catalytic-subunit-antibody-6196-immunohistochemistry-paraffin-2611202518324522.jpg)