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![Western Blot: RPA14 Antibody (14.1) [NB100-160] - Sample (30 ug of whole cell lysate) A: Molt-4 15% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RPA14-Antibody-14-1-Western-Blot-NB100-160-img0002.jpg "Western Blot: RPA14 Antibody (14.1) [NB100-160] - Sample (30 ug of whole cell lysate) A: Molt-4 15% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-262202415355711.jpg "Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-272202485637.jpg)
![Western Blot: PARP Antibody (7C11) [NBP3-26438] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-282202411472110.jpg)
![Western Blot: HDJ2 Antibody (23C6) [NBP3-26616] - Positive Western Blot detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: HDJ2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45, 38 kDa Observed band size: 45-55 kDa](http://images.novusbio.com/fullsize/nbp3-26616_rabbit-hdj2-mab-23c6-28220241140936.jpg "Western Blot: HDJ2 Antibody (23C6) [NBP3-26616] - Positive Western Blot detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: HDJ2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45, 38 kDa Observed band size: 45-55 kDa")
![Immunocytochemistry/Immunofluorescence: HDJ2 Antibody (23C6) [NBP3-26616] - Staining of A549 cell with HDJ2 Antibody (23C6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 550-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26616_rabbit-hdj2-mab-23c6-28220241312237.jpg)
![Flow Cytometry: HDJ2 Antibody (23C6) [NBP3-26616] - Overlay Peak curve showing A549 cells stained with HDJ2 Antibody (23C6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26616_rabbit-hdj2-mab-23c6-282202414312719.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Western-Blot-NBP2-42859-img0003.jpg "Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000")
![Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody (NBP2-42859) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42859_rabbit-polyclonal-histone-h4-monomethyl-lys20-antibody-immunocytochemistry-immunofluorescence-261120251847412.jpg)
![Western Blot: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] (lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Observed Molecular Weight at ~95 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60648-img0003.jpg "Western Blot: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] (lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Observed Molecular Weight at ~95 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from L1210 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] used for IP at 3 ug per reaction. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibody from Company B. For blotting immunoprecipitated NBS1, NB100-60648 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Band detected at ~95 kDa.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunoprecipitation-NB100-60648-img0004.jpg)
![Western Blot: ASC1 Antibody [NB100-419] - Detection of Human TRIP4/ASC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody NB100-419 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NB100-419-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ASC1 Antibody [NB100-419] - Detection of Human TRIP4/ASC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody NB100-419 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: ASC1 Antibody [NB100-419] - Detection of human ASC1 in Western Blot and immunoprecipitation, using NB100-419. Immunoprecipitated ASC1 was detected using an antibody to a distinctly different epitope on ASC1.](http://images.novusbio.com/fullsize/ASC1-Antibody-Western-Blot-NB100-419-img0001.jpg)
![Western Blot: SMC2 Antibody [NB100-372] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-372-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-372] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.")
![<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-372] - Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-372-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-372] - Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-372-img0003.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TMS715) [NBP2-34446] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TMS715).](http://images.novusbio.com/fullsize/Thymidylate-Synthase-Antibody-TMS715-Immunohistochemistry-Paraffin-NBP2-34446-img0002.jpg "Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TMS715) [NBP2-34446] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (TMS715).")
![Immunohistochemistry-Paraffin: TDG Antibody (311) [NBP2-43802] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TDG-Antibody-311-Immunohistochemistry-Paraffin-NBP2-43802-img0002.jpg "Immunohistochemistry-Paraffin: TDG Antibody (311) [NBP2-43802] - TDG antibody detects TDG protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain. TDG stained by TDG antibody diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: TDG Antibody (311) [NBP2-43802] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody [GT311] (NBP2-43802) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43802_mouse-monoclonal-tdg-antibody-311-910202419593596.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody [GT311] (NBP2-43802) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43802_mouse-monoclonal-tdg-antibody-311-western-blot-2611202518393111.jpg)