| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88961-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26406-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26486-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26631-50ul | |||
![Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0052.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC5 Antibody [NB100-235]](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0001.jpg)
![Western Blot: RFC5 Antibody [NB100-235] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/RFC5-Antibody-Western-Blot-NB100-235-img0002.jpg)
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)
![Western Blot: RBM14 Antibody [NBP2-20102] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RBM14 antibody [N1N2], N-term (NBP2-20102) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20102_rabbit-polyclonal-rbm14-antibody-9102024202469.jpg "Western Blot: RBM14 Antibody [NBP2-20102] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RBM14 antibody [N1N2], N-term (NBP2-20102) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry: RBM14 Antibody [NBP2-20102] - Zgc:85696 antibody detects Zgc:85696 protein on zebrafish by whole mount immunohistochemical analysis. <BR/>Sample: 2 days-post-fertilization zebrafish embryo. <BR/>Zgc:85696 antibody (NBP2-20102) dilution: 1:50.<BR/>](http://images.novusbio.com/fullsize/nbp2-20102_rabbit-polyclonal-rbm14-antibody-91020242075958.jpg)
![Immunohistochemistry: RBM14 Antibody [NBP2-20102] - Zgc:85696 antibody detects Zgc:85696 protein on zebrafish by whole mount immunohistochemical analysis. <BR/>Sample: 2 days-post-fertilization zebrafish embryo. <BR/>Zgc:85696 antibody (NBP2-20102) dilution: 1:50.<BR/>](http://images.novusbio.com/fullsize/nbp2-20102_rabbit-polyclonal-rbm14-antibody-91020242041273.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP1-32783] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Western-Blot-NBP1-32783-img0005.jpg "Western Blot: eIF2B epsilon Antibody [NBP1-32783] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody [NBP1-32783] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32783-img0003.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP1-32783] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Western-Blot-NBP1-32783-img0004.jpg)
![Western Blot: PARP4 Antibody [NBP1-06563] - Whole cell lysate from HeLa cells. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody NBP1-06562, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PARP4-Antibody-Western-Blot-NBP1-06563-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PARP4 Antibody [NBP1-06563] - Whole cell lysate from HeLa cells. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody NBP1-06562, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PARP4 Antibody [NBP1-06563] - Human colon carcinoma. Antibody: Affinity purified rabbit anti- vPARP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PARP4-Antibody-Immunohistochemistry-Paraffin-NBP1-06563-img0006.jpg)
![Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP1-88961-img0005.jpg "Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: PPP2R5C Antibody [NBP1-88961] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Immunohistochemistry-Paraffin-NBP1-88961-img0002.jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-2622024152248.jpg "Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate.<br /> All lanes: TOR/mTOR [p Ser2448] Antibody at 0.825ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 289 KDa<br /> Observed band size: 289 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241140938.jpg)
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Staining of Hela cells with TOR/mTOR [p Ser2448] Antibody (1G6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241312233.jpg)
![Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug).<br /> Lane 3: Hela whole cell lysate (10ug).<br />](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-26220241322158.jpg "Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug).<br /> Lane 3: Hela whole cell lysate (10ug).<br />")
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-262202415253321.jpg)
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-2722024827492.jpg)
![Western Blot: Aurora B Antibody (4E5) [NBP3-26486] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate.<br /> All lanes: Aurora B Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 40, 36, 17, 35 kDa<br /> Observed band size: 40 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-282202411442635.jpg)
![Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Image of XPE/DDB2 Antibody (24E5) diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB.](http://images.novusbio.com/fullsize/nbp3-26631_rabbit-xpe-ddb2-mab-24e5-262202414471563.jpg "Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Image of XPE/DDB2 Antibody (24E5) diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB.")
![Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Image of XPE/DDB2 Antibody (24E5) diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB.](http://images.novusbio.com/fullsize/nbp3-26631_rabbit-xpe-ddb2-mab-24e5-2722024820396.jpg)
![Western Blot: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Positive Western Blot detected in: PC-3 whole cell lysate.<br /> All lanes: XPE/DDB2 Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 47 kDa<br /> Observed band size: 47 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26631_rabbit-xpe-ddb2-mab-24e5-2822024115254.jpg)
![Flow Cytometry: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Overlay Peak curve showing A549 cells stained with XPE/DDB2 Antibody (24E5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26631_rabbit-xpe-ddb2-mab-24e5-282202414183670.jpg)
![Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533] - Samples: FFPE serial sections of human larynx squamous cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:1](http://images.novusbio.com/fullsize/XRCC1-[p-Ser461]-Antibody-Immunohistochemistry-NB100-533-img0006.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533] - Samples: FFPE serial sections of human larynx squamous cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:1")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533] - FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho XRCC1 (S461). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Ki](http://images.novusbio.com/fullsize/XRCC1-[p-Ser461]-Antibody-Immunohistochemistry-Paraffin-NB100-533-img0003.jpg)
![Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533] - Mouse Phospho XRCC1 (S461). Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461) dilution-1:250.](http://images.novusbio.com/fullsize/XRCC1-[p-Ser461]-Antibody-Immunohistochemistry-Paraffin-NB100-533-img0002.jpg)
![Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE and/or 100 uM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 used for WB at 1 ug/ml (upper panel); to detect total SMC3, another rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (50 ug) from HeLa cells treated with 500 uM EPE for 4 hours (+) or mock treated (-) cells Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody used for WB at 1 ug/ml (upper panel); to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Western-Blot-NBP2-37694-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SMC3 [p Ser103] Antibody [NBP2-37694] - Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 500 uM EPE for 4 hours (+). Antibodies: Phospho SMC3 was immunoprecipitated (IP) using rabbit anti-Phospho SMC3 (S1083) antibody. The IP reactions were either mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody was used for western blot (WB) of IPs at 1 ug/ml (upper panel); to detect total SMC3 affinity purified rabbit anti-SMC3 was used for WB of IPs at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) and 30 seconds (lower panel).](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-Immunoprecipitation-NBP2-37694-img0004.jpg)
![<b>Biological Strategies Validation. </b>SMC3 [p Ser103] Antibody [NBP2-37694] - Detection of human Phospho SMC3 (S1083) by western blot of immunoprecipitates. Samples: Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Phospho SMC3 (S1083) was immunoprecipitated using rabbit anti-Phospho SMC3 (S1083) antibody NBP2-37694 at 6 ug per reaction. Total SMC3 was immunoprecipitated with another rabbit anti-SMC3 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC3-[p-Ser103]-Antibody-NBP2-37694-img0005.jpg)