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![Immunoprecipitation:GARS AntibodyNBP3-29898] -Detection of human GARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GARS antibody NBP3-29898 (lot NBP3-29898-1) used for IP at 6 µg per reaction. GARS was also immunoprecipitated by rabbit anti-GARS antibody. For blotting immunoprecipitated GARS, NBP3-29898 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29898_rabbit-gars-pab-301220241318269.jpg "Immunoprecipitation:GARS AntibodyNBP3-29898] -Detection of human GARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GARS antibody NBP3-29898 (lot NBP3-29898-1) used for IP at 6 µg per reaction. GARS was also immunoprecipitated by rabbit anti-GARS antibody. For blotting immunoprecipitated GARS, NBP3-29898 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:[NBP3-29021]-Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. RPSA was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated RPSA, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29021_rabbit-rpsa-pab-231202512101681.jpg "Immunoprecipitation:[NBP3-29021]-Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. RPSA was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated RPSA, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:RPSA AntibodyNBP3-29593] -Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPSA antibody NBP3-29593 (lot NBP3-29593-1) used for IP at 6 µg per reaction. RPSA was also immunoprecipitated by rabbit anti-RPSA.. For blotting immunoprecipitated RPSA, NBP3-29593 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29593_rabbit-rpsa-pab-301220241556510.jpg "Immunoprecipitation:RPSA AntibodyNBP3-29593] -Detection of human RPSA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPSA antibody NBP3-29593 (lot NBP3-29593-1) used for IP at 6 µg per reaction. RPSA was also immunoprecipitated by rabbit anti-RPSA.. For blotting immunoprecipitated RPSA, NBP3-29593 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:[NBP3-29673]-Detection of human SNX5 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNX5 antibody used for IP at 6 µg per reaction. SNX5 was also immunoprecipitated by rabbit anti-SNX5 antibody. For blotting immunoprecipitated SNX5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29673_rabbit-snx5-pab-23120251252850.jpg "Immunoprecipitation:[NBP3-29673]-Detection of human SNX5 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNX5 antibody used for IP at 6 µg per reaction. SNX5 was also immunoprecipitated by rabbit anti-SNX5 antibody. For blotting immunoprecipitated SNX5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:Glyoxalase I AntibodyNBP3-29592] -Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase Iantibody NBP3-29592 (lot NBP3-29592-1) used for IP at 6 µg per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I.. For blotting immunoprecipitated GLO1/Glyoxalase I, NBP3-29592 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-29592_rabbit-glyoxalase-i-pab-3012202412262520.jpg "Immunoprecipitation:Glyoxalase I AntibodyNBP3-29592] -Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase Iantibody NBP3-29592 (lot NBP3-29592-1) used for IP at 6 µg per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I.. For blotting immunoprecipitated GLO1/Glyoxalase I, NBP3-29592 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunohistochemistry:[NBP3-29204]-Detection of human MCM5 by immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-MCM5.](http://images.novusbio.com/fullsize/nbp3-29204_rabbit-mcm5-pab-23120251225662.jpg "Immunohistochemistry:[NBP3-29204]-Detection of human MCM5 by immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-MCM5.")
![Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30522_rabbit-blooms-syndrome-protein-blm-pab-2312025129511.jpg "Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249342744.jpg "Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249374967.jpg)
![Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0001.jpg)
![Western Blot:[NBP3-29727]-Detection of human and mouse Cytochrome C by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytochrome C antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29727_rabbit-cytochrome-c-pab-23120251252876.jpg "Western Blot:[NBP3-29727]-Detection of human and mouse Cytochrome C by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytochrome C antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation:Cyclin B2 AntibodyNBP3-30035] -Detection of human Cyclin B2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin B2 antibody NBP3-30035 (lot NBP3-30035-1) used for IP at 6 µg per reaction. Cyclin B2 was also immunoprecipitated by rabbit anti-Cyclin B2 antibody. For blotting immunoprecipitated Cyclin B2, BL17041 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30035_rabbit-cyclin-b2-pab-3012202412392130.jpg "Immunoprecipitation:Cyclin B2 AntibodyNBP3-30035] -Detection of human Cyclin B2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin B2 antibody NBP3-30035 (lot NBP3-30035-1) used for IP at 6 µg per reaction. Cyclin B2 was also immunoprecipitated by rabbit anti-Cyclin B2 antibody. For blotting immunoprecipitated Cyclin B2, BL17041 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.")
![<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody (NBP3-12918) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0001.jpg)