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![Western Blot: RUVBL1 Antibody [NBP2-20244] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-20244-img0001.jpg "Western Blot: RUVBL1 Antibody [NBP2-20244] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Western Blot: RUVBL1 Antibody [NBP2-20244] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20244_rabbit-polyclonal-ruvbl1-antibody-1610202419405763.jpg)
![Western Blot: Cdc20 Antibody [NBP2-15825] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NBP2-15825-img0001.jpg "Western Blot: Cdc20 Antibody [NBP2-15825] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: MAD2L1 Antibody [NBP1-33529] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [N1C3].](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-33529-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-33529] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [N1C3].")
![<b>Genetic Strategies Validation. </b>Western Blot: MAD2L1 Antibody [NBP1-33529] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Western-Blot-NBP1-33529-img0004.jpg)
![Western Blot: EBP1 Antibody [NBP2-16256] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16256-img0001.jpg "Western Blot: EBP1 Antibody [NBP2-16256] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE gel, diluted at 1:10000.")
![Immunoprecipitation: PA2G4/EBP1 Antibody [NBP2-16256] - EBP1 antibody immunoprecipitates EBP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of EBP1 protein by 2 ug of EBP1 antibody 7.5% SDS-PAGE. The immunoprecipitated EBP1 protein was detected by EBP1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunoprecipitation-NBP2-16256-img0003.jpg)
![Western Blot: LSM4 Antibody [NBP2-17182] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LSM4 antibody [N1C3] (NBP2-17182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17182_rabbit-polyclonal-lsm4-antibody-9102024205627.jpg "Western Blot: LSM4 Antibody [NBP2-17182] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LSM4 antibody [N1C3] (NBP2-17182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: EIF2 beta Antibody [NB100-93283] - Detection of Human and Mouse eIF2beta/EIF2S2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit antieIF2beta/ EIF2S2 antibody NB100-93283 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated more efficiently by rabbit anti-eIF2beta/EIF2S2 antibody NB100-93284, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/EIF2-beta-Antibody-Western-Blot-NB100-93283-img0001.jpg "Western Blot: EIF2 beta Antibody [NB100-93283] - Detection of Human and Mouse eIF2beta/EIF2S2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit antieIF2beta/ EIF2S2 antibody NB100-93283 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated more efficiently by rabbit anti-eIF2beta/EIF2S2 antibody NB100-93284, which recognizes a downstream epitope.")