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![SDS-Page: Recombinant Human, 53BP1 Protein [H00007158-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue. A band appears approximately at the theoretical molecular weight of 38.7 kDa.](http://images.novusbio.com/fullsize/53BP1-Partial-Recombinant-Protein-SDS-Page-H00007158-Q01-img0001.jpg "SDS-Page: Recombinant Human, 53BP1 Protein [H00007158-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue. A band appears approximately at the theoretical molecular weight of 38.7 kDa.")
![SDS-Page: Recombinant Human ATRX Protein [H00000546-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ATRX-Protein-SDS-Page-H00000546-Q01-img0001.jpg "SDS-Page: Recombinant Human ATRX Protein [H00000546-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human, Nbs1 Protein [H00004683-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue, using Recombinant Human, Nbs1 Protein [H00004683-Q01].](http://images.novusbio.com/fullsize/qc_test-H00004683-Q01-1.jpg "SDS-Page: Recombinant Human, Nbs1 Protein [H00004683-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue, using Recombinant Human, Nbs1 Protein [H00004683-Q01].")
![SDS-Page: Recombinant Human ATM Protein [H00000472-Q01] - Analysis in 12.5% SDS-PAGE Stained with Coomassie Blue using Recombinant Human ATM Protein [H00000472-Q01].](http://images.novusbio.com/fullsize/Recombinant-Human-ATM-Protein-SDS-Page-H00000472-Q01-img0001.jpg "SDS-Page: Recombinant Human ATM Protein [H00000472-Q01] - Analysis in 12.5% SDS-PAGE Stained with Coomassie Blue using Recombinant Human ATM Protein [H00000472-Q01].")
![Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32754_rabbit-chk1-p-ser280-mab-je47-66-288202412442937.jpg "Western Blot: Chk1 [p Ser280] Antibody (JE47-66) [NBP3-32754] - Western blot analysis of Chk1 on different lysates with Rabbit anti-Chk1 antibody (NBP3-32754) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32754) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32756_rabbit-chk2-p-thr68-mab-je43-12-288202412442927.jpg "Western Blot: Chk2 [p Thr68] Antibody (JE43-12) [NBP3-32756] - Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (NBP3-32756) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20uM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25uM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25uM Etoposide for 5 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32756) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32890_rabbit-rad50-mab-je60-04-288202412461633.jpg "Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: U-937 cell lysate (20 ug/Lane) Lane 5: Caco-2 cell lysate (20 ug/Lane) Lane 6: Mouse testis tissue lysate (40 ug/Lane) Lane 7: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")