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![Western Blot: Chk1 Antibody [NBP3-30982] - Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-30982_sheep-chk1-pab-811202420592127.jpg "Western Blot: Chk1 Antibody [NBP3-30982] - Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.")
![Western Blot: PPP2R5C Antibody [NBP3-30779] - Western blot analysis using anti-PP2A/B gamma 2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2).](http://images.novusbio.com/fullsize/nbp3-30779_rabbit-ppp2r5c-pab-811202420592180.jpg "Western Blot: PPP2R5C Antibody [NBP3-30779] - Western blot analysis using anti-PP2A/B gamma 2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2).")
![Western Blot: TLK1 Antibody [NBP1-77982] - Shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP1-77982-img0002.jpg "Western Blot: TLK1 Antibody [NBP1-77982] - Shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.")
![Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-Western-Blot-NBP1-77981-img0003.jpg "Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.")
![N/A: Rad9 [p Ser1260] Antibody [NBP1-77981]](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-N-A-NBP1-77981-img0002.jpg)
![Immunohistochemistry:[NBP3-29226]-Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-SMC1.](http://images.novusbio.com/fullsize/nbp3-29226_rabbit-smc1-pab-2312025128832.jpg "Immunohistochemistry:[NBP3-29226]-Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-SMC1.")
![Immunohistochemistry:[NBP3-29326]-Detection of human Phospho SMC1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Mock treatment or lambda phosphatase-treatment. Antibody: Affinity purified rabbit anti-Phospho-SMC1.](http://images.novusbio.com/fullsize/nbp3-29326_rabbit-smc1-pab-2312025129512.jpg "Immunohistochemistry:[NBP3-29326]-Detection of human Phospho SMC1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Mock treatment or lambda phosphatase-treatment. Antibody: Affinity purified rabbit anti-Phospho-SMC1.")
![Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0001.jpg)
![Western Blot: ATRIP Antibody (OTI1G6) [NBP2-46557] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ATRIP-Antibody-1G6-Western-Blot-NBP2-46557-img0001.jpg "Western Blot: ATRIP Antibody (OTI1G6) [NBP2-46557] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Western Blot: ATRIP Antibody (OTI1G6) [NBP2-46557] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP.](http://images.novusbio.com/fullsize/ATRIP-Antibody-1G6-Western-Blot-NBP2-46557-img0002.jpg)
![Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NB100-57077-img0001.jpg "Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Choline kinase alpha Antibody [NBP1-56668] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Choline-kinase-alpha-Antibody-Western-Blot-NBP1-56668-img0003.jpg "Western Blot: Choline kinase alpha Antibody [NBP1-56668] - Sample Type: Human Adult Placenta Antibody Dilution: 1.0 ug/ml")
![Western Blot: Choline kinase alpha Antibody [NBP1-56668] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Human Liver](http://images.novusbio.com/fullsize/Choline-kinase-alpha-Antibody-Western-Blot-NBP1-56668-img0004.jpg)
![Western Blot: PERP Antibody [NBP1-62434] - PANC1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-62434-img0002.jpg "Western Blot: PERP Antibody [NBP1-62434] - PANC1 cell lysate, concentration 0.2-1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F6A9-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12825-img0001.jpg "Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.")
![Flow Cytometry: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Flow cytometry analysis of PD-L1 transfected 293 cells using PD-L1 [F6A9] antibody at 10 ug/ml. Blue: untransfected cells, Yellow: PD-L1 transfected cells.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F6A9-VHH-Flow-Cytometry-NBP3-12825-img0002.jpg)