| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50231-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26386-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26494-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22663-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21602-50ul | |||
![Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-29820241492673.jpg "Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.](http://images.novusbio.com/fullsize/p53-Antibody-V5P4H6-B3-Immunohistochemistry-NBP2-50231-img0001.jpg "Immunohistochemistry: p53 Antibody (V5P4H6/B3) [NBP2-50231] - Analysis of zebra fish embryo.")
![Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-274-img0133.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-274] - Whole cell lysate (30 ug) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-274-img0132.jpg)
![Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate).<br /> All lanes: p53 [p Thr55] Antibody at 1.28ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 53 KDa<br /> Observed band size: 53 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202411351620.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Positive Western Blot detected in 293 whole cell lysate (treated with Calyculin A or Pervanadate).<br /> All lanes: p53 [p Thr55] Antibody at 1.28ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 53 KDa<br /> Observed band size: 53 KDa<br />")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 [p Thr55] Antibody (3C3) [NBP3-26386] - Staining of 293 cells (treated with 50mM Calyculin A for 30min) with p53 [p Thr55] Antibody (3C3) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26386_rabbit-p53-p-thr-55-mab-3c3-282202412493626.jpg)
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-262202415355749.jpg "Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATM Antibody (3G11) [NBP3-26494] - Image of ATM Antibody (3G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-27220248313715.jpg)
![Flow Cytometry: ATM Antibody (3G11) [NBP3-26494] - Overlay histogram showing Hela cells stained with ATM Antibody (3G11) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26494_rabbit-atm-mab-3g11-282202414312732.jpg)
![Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Western blot detection of Histone H2AX in Hela, Ramos using NBP3-20068 (1:1000 diluted)](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-S02-3C8-Western-Blot-NBP3-20068-img0002.jpg "Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Western blot detection of Histone H2AX in Hela, Ramos using NBP3-20068 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Immunohistochemistry of Histone H2AX in paraffin-embedded Human lung cancer tissue using NBP3-20068 at dilution 1/50](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-S02-3C8-Immunohistochemistry-Paraffin-NBP3-20068-img0001.jpg)
![Western Blot: Nbs1 [p Ser 343] Antibody (SR2302) [NBP3-22663] - Western blot analysis using NBP3-22663 in Jurkat cell lysate treated with Etopside.](http://images.novusbio.com/fullsize/nbp3-22663_rabbit-nbs1-p-ser-343-mab-sr2302-57202317215135.jpg "<b>Biological Strategies Validation. </b>Western Blot: Nbs1 [p Ser 343] Antibody (SR2302) [NBP3-22663] - Western blot analysis using NBP3-22663 in Jurkat cell lysate treated with Etopside.")
![Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot detection of Chk1 in K562 cell lysates using Chk1 Rabbit mAb (1:1000 diluted). Predicted band size: 54KDa. Observed band size: 54KDa.](http://images.novusbio.com/fullsize/Chk1-Antibody-S07-4K2-Western-Blot-NBP3-14889-img0001.jpg "Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot detection of Chk1 in K562 cell lysates using Chk1 Rabbit mAb (1:1000 diluted). Predicted band size: 54KDa. Observed band size: 54KDa.")
![Western Blot: Chk1 Antibody (S07-4K2) [NBP3-14889] - Western blot of K562, 3T3, Hela cell lysates using NBP3-14889. Predicted band size: 54kDa. Observed band size: 54kDa.](http://images.novusbio.com/fullsize/nbp3-14889_rabbit-chk1-mab-s07-4k2-103202523464531.jpg)
![Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21602_rabbit-rad17-p-ser-656-mab-sr2259-67202310620.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser 656] Antibody (SR2259) [NBP3-21602] - Western blot analysis using NBP3-21602 in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate.")