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![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB110-93480-img0001.jpg "Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.")
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Showing specific labeling of GFAP (red) and vimentin (green) cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000, green), colabeled with anti-MeCP2 (1:500, red), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunofluorescence of a section of rat cerebellum labeled with anti-GFAP (1:5000,green), colabeled with anti-calbindin (1:2,000, red), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB110-93480] - Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (1:1000, green) and anti-MAP2 (1:1000, red).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-93480-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Immunohistochemistry-Paraffin-NBP2-37741-img0005.jpg "Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human breast cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Immunohistochemistry-Paraffin-NBP2-37741-img0006.jpg)
![Flow Cytometry: CD133 Antibody (3F10) [NBP2-37741] - Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Flow-Cytometry-NBP2-37741-img0002.jpg)
![ELISA: CD133 Antibody (3F10) [NBP2-37741] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-ELISA-NBP2-37741-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD133 Antibody (3F10) [NBP2-37741] - Upregulations of TIPRL, LC3 and CD133 in HCCs. Human tissues were stained with the indicated antibodies followed by confocal observation. The representative images were selected from normal and HCCs groups, respectively. DAPI was used for nucleus staining, and scar bars, 20 (inserted) and 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53191-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Immunocytochemistry-Immunofluorescence-NBP2-37741-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) - BSA Free [NBP2-37741] - Significant correlations between levels of TIPRL, LC3 & CD133 in liver cancers. (a–c) The statistically significant associations between levels of TIPRL, LC3 & CD133 in each grade of liver cancers were determined by the Spearman correlation. Each dot indicates a single patient. (d) The representative images were selected from each grade of human liver cancer tissues that were stained with indicated antibodies. DAPI was used for nucleus staining, & scar bar, 100 μm. (e) Quantitative analyses of TIPRL, LC3 & CD133 were conducted according to each grade of liver cancers. (n), the number of samples is indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31727942), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37741_mouse-monoclonal-cd133-antibody-3f10-310202415371917.jpg)
![Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Western-Blot-NBP2-72740-img0009.jpg "Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP-13.")
![Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Anti-MMP13 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72740-img0008.jpg)
![Western Blot: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of extracts (10ug) from 5 different cell lines by using anti-MMP13 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Western-Blot-NBP2-72740-img0001.jpg)
![Immunohistochemistry-Paraffin: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72740-img0007.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0010.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0011.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0012.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0013.jpg)
![Immunohistochemistry: MMP-13 Antibody (OTI2D8) - Azide and BSA Free [NBP2-72740] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MMP-13-Antibody-OTI2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72740-img0015.jpg)
![Western Blot: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta 1 Tubulin.](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Western-Blot-NBP2-72225-img0002.jpg "Western Blot: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta 1 Tubulin.")
![Immunocytochemistry/Immunofluorescence: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta I Tubulin.](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72225-img0008.jpg)
![Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72225-img0007.jpg)
![Western Blot: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Western-Blot-NBP2-72225-img0001.jpg)
![Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72225-img0003.jpg)
![Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72225-img0004.jpg)
![Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72225-img0005.jpg)
![Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) - Azide and BSA Free [NBP2-72225] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-I-Tubulin-Antibody-OTI4A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72225-img0006.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0002.jpg "Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0008.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0007.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0009.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0006.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Western-Blot-NBP2-70078-img0002.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70078-img0004.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0008.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Alpha Actinin 1 antibody (7A4) at 1:2000 + recombinant human Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Western-Blot-NBP2-70078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70078-img0003.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0005.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0006.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0007.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0009.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0006.jpg "Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunocytochemistry-Immunofluorescence-NBP2-67540-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Flow-Cytometry-NBP2-67540-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0004.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0008.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0009.jpg "Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Western blot analysis of MUC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0008.jpg)
![Flow Cytometry: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Flow-Cytometry-NBP2-67650-img0001.jpg)
![Western Blot: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Western-Blot-NBP2-67650-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody (SN06-80) [NBP2-67650] - Staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunocytochemistry-Immunofluorescence-NBP2-67650-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody (SN06-80) [NBP2-67650] - Analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC1-Antibody-SN06-80-Immunohistochemistry-Paraffin-NBP2-67650-img0005.jpg)
![Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY-5Y cell lysateLane 2: SKBR3 cell lysate](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Western-Blot-NBP2-66961-img0011.jpg "Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY-5Y cell lysateLane 2: SKBR3 cell lysate")
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0010.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0014.jpg)
![Flow Cytometry: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Flow-Cytometry-NBP2-66961-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0006.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0008.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0009.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunofluorescence staining of paraffin- embedded human colon using anti-MUC2 rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/100 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0012.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0013.jpg)
![Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1:500 dilution. Positive control:Lane 1: Mouse colon. Lane 2: Human small intestine. Lane 3: Human colon. Lane 4: Rat kidney.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Western-Blot-NBP2-75707-img0008.jpg "Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1:500 dilution. Positive control:Lane 1: Mouse colon. Lane 2: Human small intestine. Lane 3: Human colon. Lane 4: Rat kidney.")
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0004.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0007.jpg)
![Flow Cytometry: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of HeLa cells with Villin1 antibody at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Flow-Cytometry-NBP2-75707-img0001.jpg)
![Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Caco-2 cell line lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Western-Blot-NBP2-75707-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunocytochemistry-Immunofluorescence-NBP2-75707-img0003.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0005.jpg)
![Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Villin-1-Antibody-JU34-75-Immunohistochemistry-Paraffin-NBP2-75707-img0006.jpg)