| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90577-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98732-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90382-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99737-50ul | |||
![Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD48-SLAMF2-Antibody-341-Flow-Cytometry-NBP2-90577-img0001.jpg "Flow Cytometry: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Flow cytometric analysis of Mouse CD48/SLAMF2 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48/SLAMF2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: CD48/SLAMF2 Antibody (341) [NBP2-90577] - Immunofluorescence staining of Mouse CD48/SLAMF2 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90577 antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/nbp2-90577_rabbit-monoclonal-cd48-slamf2-antibody-341-235202514331563.png)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Immunochemical staining of human p63/TP73L in human breast carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-143-Immunohistochemistry-Paraffin-NBP2-89212-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Immunochemical staining of human p63/TP73L in human breast carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Staining of human P63 in human prostatic carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-143-Immunohistochemistry-Paraffin-NBP2-89212-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (143) [NBP2-89212] - Staining of human P63 in human prostate with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-143-Immunohistochemistry-Paraffin-NBP2-89212-img0002.jpg)
![Western Blot: Rab7a Antibody [NBP2-98732] - Lane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-98732_rabbit-polyclonal-rab7a-antibody-western-blot-81120237728..jpg "Western Blot: Rab7a Antibody [NBP2-98732] - Lane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa")
![Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-98732] - Immunofluorescence staining of Rab7a in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98732 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98732_rabbit-polyclonal-rab7a-antibody-235202516335043.png)
![Western Blot: Pericentrin Antibody (PSH01-66) [NBP3-32737] - Western blot analysis of Pericentrin on different lysates with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 378 kDa Observed band size: 460 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32737) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32737_rabbit-pericentrin-mab-psh01-66-278202416331247.jpg "Western Blot: Pericentrin Antibody (PSH01-66) [NBP3-32737] - Western blot analysis of Pericentrin on different lysates with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 378 kDa Observed band size: 460 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32737) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody (PSH01-66) [NBP3-32737] - Immunocytochemistry analysis of HeLa cells labeling Pericentrin with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pericentrin antibody (NBP3-32737) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32737_rabbit-pericentrin-mab-psh01-66-29202410234213.jpg)
![Western Blot: Villin 1 Antibody (C4) [NBP3-33090] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33090_rabbit-villin-1-mab-c4-288202414191528.jpg "Western Blot: Villin 1 Antibody (C4) [NBP3-33090] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Villin 1 Antibody (C4) [NBP3-33090] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin 1 antibody (NBP3-33090) at 1/3,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33090) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33090_rabbit-villin-1-mab-c4-29820241416187.jpg)
![Immunohistochemistry: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32614) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32614_rabbit-ca125-muc16-mab-pd01-13-29820241358558.jpg "Immunohistochemistry: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32614) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CA125/MUC16 Antibody (PD01-13) [NBP3-32614] - Immunocytochemistry analysis of SKOV-3 cells labeling CA125/MUC16 with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CA125/MUC16 antibody (NBP3-32614) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32614_rabbit-ca125-muc16-mab-pd01-13-29202410484871.jpg)
![Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-278202416331273.jpg "Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32198) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-29820241416182.jpg)
![Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody (052) [NBP2-90382] - Staining of mouse SCARB2 in mouse brain with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-052-Immunohistochemistry-Paraffin-NBP2-90382-img0002.jpg "Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody (052) [NBP2-90382] - Staining of mouse SCARB2 in mouse brain with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody (052) [NBP2-90382] - Staining of mouse SCARB2 in mouse kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-052-Immunohistochemistry-Paraffin-NBP2-90382-img0001.jpg)
![Western Blot: Villin 1 Antibody (A2) [NBP3-33091] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33091) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33091_rabbit-villin-1-mab-a2-28820241419152.jpg "Western Blot: Villin 1 Antibody (A2) [NBP3-33091] - Western blot analysis of Villin 1 on HT-29 cell lysates with Rabbit anti-Villin 1 antibody (NBP3-33091) at 1/2,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 93 kDa Observed band size: 85 kDa Exposure time: 15 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Villin 1 Antibody (A2) [NBP3-33091] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin 1 antibody (NBP3-33091) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33091) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33091_rabbit-villin-1-mab-a2-29820241420928.jpg)
![Western Blot: CD48/SLAMF2 Antibody (JE37-98) [NBP3-32157] - Western blot analysis of CD48/SLAMF2 on different lysates with Rabbit anti-CD48/SLAMF2 antibody (NBP3-32157) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27.7 kDa Observed band size: 40 kDa Exposure time: 80 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32157) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32157_rabbit-cd48-slamf2-mab-je37-98-2782024163160.jpg "Western Blot: CD48/SLAMF2 Antibody (JE37-98) [NBP3-32157] - Western blot analysis of CD48/SLAMF2 on different lysates with Rabbit anti-CD48/SLAMF2 antibody (NBP3-32157) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27.7 kDa Observed band size: 40 kDa Exposure time: 80 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32157) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD48/SLAMF2 Antibody (JE37-98) [NBP3-32157] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48/SLAMF2 antibody (NBP3-32157) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32157_rabbit-cd48-slamf2-mab-je37-98-29820241435245.jpg)
![Immunohistochemistry-Paraffin: c-Abl Antibody [NBP2-99737] - Immunochemical staining of human c-Abl in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunohistochemistry-Paraffin-NBP2-99737-img0002.jpg "Immunohistochemistry-Paraffin: c-Abl Antibody [NBP2-99737] - Immunochemical staining of human c-Abl in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP2-99737] - Immunofluorescence staining of c-Abl in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99737 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99737_rabbit-polyclonal-c-abl-antibody-235202511354145.png)
![Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32445_rabbit-hsp70-hspa1a-mab-pd01-65-278202416331233.jpg "Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32445_rabbit-hsp70-hspa1a-mab-pd01-65-298202413585585.jpg)