| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34442-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-262SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26216-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65351SS | |||
| Azide and BSA Free | NBP2-80648 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54464-100ug | |||
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP1-31118-img0003.jpg "Western Blot: COL4A3BP Antibody [NBP1-31118] - Sample (50 ug of whole cell lysate) A: Mouse white adipose 7. 5% SDS PAGE diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: COL4A3BP Antibody [NBP1-31118] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31118-img0002.jpg)
![Immunohistochemistry-Paraffin: COL4A3BP Antibody [NBP1-31118] - BT483 xenograft. COL4A3BP antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Immunohistochemistry-Paraffin-NBP1-31118-img0004.jpg)
![Western Blot: COL4A3BP Antibody [NBP1-31118] - A: Hep G2 B: MOLT4 C: Raji 12% SDS PAGE; antibody diluted at 1:500](http://images.novusbio.com/fullsize/COL4A3BP-Antibody-Western-Blot-NBP1-31118-img0001.jpg)
![Western Blot: Bcl-2 Antibody (SPM530) [NBP2-34389] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM530).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM530-Western-Blot-NBP2-34389-img0004.jpg "Western Blot: Bcl-2 Antibody (SPM530) [NBP2-34389] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM530).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (SPM530) [NBP2-34389] - Formalin-fixed, paraffin-embedded tonsil stained with Bcl-2 Mouse Monoclonal Antibody (SPM530).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM530-Immunohistochemistry-Paraffin-NBP2-34389-img0005.jpg)
![Western Blot: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30474-img0005.jpg "Western Blot: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30474] - Formaldehyde-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:100 (2ug/ml) .](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30474-img0002.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BRD2 Antibody [NBP1-30474] - Whole cell lysate from HeLa and 293T cells. NBP1-30474 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. BRD2 was also IPed by rabbit anti-BRD2 antibody NBP1-30475.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30474-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30475 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP1-30474 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30474-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 (lot NBP1-30474-2) used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30475. For blotting immunoprecipitated BRD2, NBP1-30474 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30474-img0006.jpg)
![Immunohistochemistry-Paraffin: gamma Catenin Antibody [NBP2-16600] - : Paraffin-embedded human colon carcinoma. gamma Catenin antibody [C2C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP2-16600-img0003.jpg "Immunohistochemistry-Paraffin: gamma Catenin Antibody [NBP2-16600] - : Paraffin-embedded human colon carcinoma. gamma Catenin antibody [C2C3], C-term dilution: 1:500.")
![Immunohistochemistry-Paraffin: gamma Catenin Antibody [NBP2-16600] - Paraffin-embedded mouse esophagus. gamma Catenin antibody [C2C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/gamma-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP2-16600-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: gamma Catenin Antibody [NBP2-16600] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with gamma Catenin antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16600_rabbit-polyclonal-gamma-catenin-antibody-1610202419414586.jpg)
![Western Blot: XAGE1 Antibody [NB100-262] - Detection of XAGE on purified His-XAGE (100 ug) using NB100-262.](http://images.novusbio.com/fullsize/XAGE1-Antibody-Western-Blot-NB100-262-img0003.jpg "Western Blot: XAGE1 Antibody [NB100-262] - Detection of XAGE on purified His-XAGE (100 ug) using NB100-262.")
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-262202415355731.jpg "Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-26220241522415.jpg)
![Western Blot: HSP90 beta Antibody (7G7) [NBP3-26216] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate. All lanes: HSP90 beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 84 kDa Observed band size: 80-90 kDa](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202411485820.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (7G7) [NBP3-26216] - Staining of HepG2 cell with HSP90 beta Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-28220241312214.jpg)
![Flow Cytometry: HSP90 beta Antibody (7G7) [NBP3-26216] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7G7) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202414312789.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Flow Cytometry: CD4 Antibody (OX-38) [NB100-65351] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-65351) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-OX-38-Flow-Cytometry-NB100-65351-img0001.jpg "Flow Cytometry: CD4 Antibody (OX-38) [NB100-65351] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-65351) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with B220/CD45R Antibody (PTPRC/1461) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1461-Immunocytochemistry-Immunofluorescence-NBP2-53174-img0004.jpg "Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with B220/CD45R Antibody (PTPRC/1461) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Monoclonal Antibody (PTPRC/1461). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1461-Immunohistochemistry-Paraffin-NBP2-53174-img0005.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1461) [NBP2-53174] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using B220/CD45R Antibody (PTPRC/1461) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1461-Flow-Cytometry-NBP2-53174-img0003.jpg)
![Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP1-32748] - Porcine skeletal muscle, using Troponin T1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Troponin-T-type-1-slow-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP1-32748-img0003.jpg "Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP1-32748] - Porcine skeletal muscle, using Troponin T1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Troponin T type 1 (slow skeletal) Antibody [NBP1-32748] - Human colon carcinoma, using Troponin T1 (slow skeletal) antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Troponin-T-type-1-slow-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP1-32748-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Actin-Regulatory-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-15259-img0002.jpg "Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.")
![Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Actin-Regulatory-Protein-Antibody-Western-Blot-NBP2-15259-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP2-15259] - CapG antibody [N3C3] detects CapG protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CapG protein stained by CapG antibody [N3C3] (NBP2-15259) diluted at 1:400.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15259_rabbit-polyclonal-capg-antibody-910202420104435.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CapG Antibody [NBP2-15259] - Wild-type (WT) and CAPG knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CAPG antibody [N3C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15259_rabbit-polyclonal-capg-antibody-1610202419414516.jpg)