| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03303-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94163-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03913-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59466-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59500-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03298-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35743-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59388-50ul | |||
![Western Blot: UbcH10/UBE2C Antibody [NBP3-03303] - Analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UbcH10/UBE2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-Knockout-Validated-NBP3-03303-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: UbcH10/UBE2C Antibody [NBP3-03303] - Analysis of extracts from normal (control) and UBE2C knockout (KO) HeLa cells, using UbcH10/UBE2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-33562_rabbit-cdc37-p-ser13-mab-5p8b2-912202415152871.jpg "Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3min.")
![Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of various lysates using CDC37 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33562_rabbit-cdc37-p-ser13-mab-5p8b2-912202415111821.jpg)
![Western Blot: SPECC1L Antibody [NBP2-94163] - Analysis of extracts of various cell lines, using SPECC1L at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.](http://images.novusbio.com/fullsize/SPECC1L-Antibody-Western-Blot-NBP2-94163-img0001.jpg "Western Blot: SPECC1L Antibody [NBP2-94163] - Analysis of extracts of various cell lines, using SPECC1L at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.")
![Immunohistochemistry-Paraffin: SPECC1L Antibody [NBP2-94163] - Immunohistochemistry of paraffin-embedded Human esophageal using SPECC1L Rabbit pAb (NBP2-94163) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SPECC1L-Antibody-Immunohistochemistry-Paraffin-NBP2-94163-img0002.jpg)
![Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Western-Blot-NBP3-16353-img0002.jpg "Western Blot: Chk1 Antibody (0S3Q1) [NBP3-16353] - Western blot analysis of extracts of various cell lines, using Chk1 antibody (NBP3-16353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Chk1 Antibody (0S3Q1) [NBP3-16353] - Immunohistochemistry of paraffin-embedded human colon using Chk1 Rabbit mAb (NBP3-16353) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Chk1-Antibody-0S3Q1-Immunohistochemistry-Paraffin-NBP3-16353-img0001.jpg)
![Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/LATS2-Antibody-Western-Blot-NBP3-03913-img0004.jpg "Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CDC37 Antibody (3G7) [NBP2-59466] - Lane 1: Jurkat cell lysates, Lane 2: A431 cell lysates, Lane 3: K562 cell lysates, Lane 4: LnCap cell lysates, Lane 5: SW480 cell lysates, Lane 6: HeLa cell lysates, Lane 7: MCF7 cell lysates, Lane 8: 293T cell lysates](http://images.novusbio.com/fullsize/CDC37-Antibody-3G7-Western-Blot-NBP2-59466-img0001.jpg "Western Blot: CDC37 Antibody (3G7) [NBP2-59466] - Lane 1: Jurkat cell lysates, Lane 2: A431 cell lysates, Lane 3: K562 cell lysates, Lane 4: LnCap cell lysates, Lane 5: SW480 cell lysates, Lane 6: HeLa cell lysates, Lane 7: MCF7 cell lysates, Lane 8: 293T cell lysates")
![Immunocytochemistry/Immunofluorescence: CDC37 Antibody (3G7) [NBP2-59466] - Analysis of CDC37 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDC37 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/CDC37-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP2-59466-img0002.jpg)
![Western Blot: CSK Antibody (7C6) [NBP2-59500] - Lane 1: 293T cell lysates, Lane 2: CSK transfected 293T cell lysates](http://images.novusbio.com/fullsize/CSK-Antibody-7C6-Western-Blot-NBP2-59500-img0001.jpg "Western Blot: CSK Antibody (7C6) [NBP2-59500] - Lane 1: 293T cell lysates, Lane 2: CSK transfected 293T cell lysates")
![Immunocytochemistry/Immunofluorescence: CSK Antibody (7C6) [NBP2-59500] - Analysis of CSK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/CSK-Antibody-7C6-Immunocytochemistry-Immunofluorescence-NBP2-59500-img0002.jpg)
![Western Blot: p18INK4c/CDKN2C Antibody [NBP3-03298] - Analysis of extracts from normal (control) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using p18INK4c/CDKN2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking Buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/p18INK4c-CDKN2C-Antibody-Knockout-Validated-NBP3-03298-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p18INK4c/CDKN2C Antibody [NBP3-03298] - Analysis of extracts from normal (control) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using p18INK4c/CDKN2C antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking Buffer: 3% nonfat dry milk in TBST.")
![Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35743_rabbit-hmgb2-pab-912202415171171.jpg "Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-6A10-Western-Blot-NBP2-59388-img0001.jpg "Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-6A10-Immunocytochemistry-Immunofluorescence-NBP2-59388-img0002.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)